Enzymes as agents for the treatment of disease

Goldberg, David M.

doi:10.1016/0009-8981(92)90007-d

Cited by 29 publications

(10 citation statements)

References 106 publications

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“…The values of these enzyme activities were within the reference range which is rather wide even for the physiological state. Our results agree with those of Vrzgula et al (1990), Fesus et al (1991 and Goldberg (1992). A common characteristics of the selected enzymes in the groups is their high variability which is most likely caused by instability of blood plasma indices.…”

Section: Discussionsupporting

confidence: 93%

“…We obtained information on changes in these indices as related to breed for the two breeds under study. Our results confirm those of Goldberg (1992) showing that the animals with higher growth rates exhibit higher activities of plasma enzymes AST, ALT and ALP as reflected not only in morphological variables such as the weight gain but also in physiological characteristics. In this context we can agree with the opinion of Nagvi et al (1991) that the phylogenetic origin of animals affects also the enzymatic values and the activity of plasma enzymes is included among those interior features which could be used to characterise the metabolic and production types of animals.…”

Section: Discussionsupporting

confidence: 90%

“…These parameters also indicate the health state and vitality of animals (J e l í n e k et al. 1987;Hooda and Nagvi 1990;Goldberg 1992;Nagvi et al 1991).…”

mentioning

confidence: 99%

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Plasma Neuromediators and The Enzymatic Profile of Sheep

Pástorová¹,

Buleca²,

Pilipčinec³

et al. 2000

Acta Vet. Brno

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Pástorová B., Buleca J., Pilipãinec, E. ·vick˘, J. Szarek: Plasma Neuromediators and the Enzymatic Profile of Sheep. Acta Vet. Brno 2000, 69: 277-280.The study focused on the plasma norepinephrine and epinephrine and activity of selected plasma enzymes and LD isoenzyme spectrum in two sheep breeds (Slovak Merino and Improved Valachian) during the anoestrous period. Radioenzymatic method was used to determine the concentrations of plasma catecholamines. Statistically significant differences were found (P < 0.05) in concentrations of norepinephrine and epinephrine in favour of the Slovak Merino breed. Significantly increased enzyme activies (P < 0.01) were observed in plasma aminotransferases ALT, AST and alkaline-phosphatase (ALP) in Slovak Merino. In improved Valachian, statistically significant changes (P < 0.01) were recorded in the activity of lactate dehydrogenase (LD). The LD isoenzyme spectrum showed statistically significant differences (P < 0.05) in the activity of isoenzyme LD2 and highly significant differences (P < 0.01) in the activity of isoenzymes LD4 and LD5 in favour of Improved Valachian sheep. These results may help to identify more accurately the metabolic constitutional and production types of sheep in different breeds.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

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Plasma Neuromediators and The Enzymatic Profile of Sheep

Pástorová¹,

Buleca²,

Pilipčinec³

et al. 2000

Acta Vet. Brno

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“…Although pharmaceutical research and development activities in the field of recombinant therapeutic proteins are mainly focused on antibodies, cytokines, and hormones rather than on enzymes, there are nevertheless three broad areas where enzymes are successfully employed in therapy: (1) to replace enzymes which are missing or defective as a consequence of inherited genetic disease; (2) to replace enzymes that are deficient or present only in inadequate quantity as a consequence of acquired disease in the organ(s) where they are normally synthesized; and (3) to provide a specific biological effect which is dependent on the catalytic activity of the enzyme [2]. Furthermore, some enzymes are used in a supportive manner in combination with other therapies, or for the removal of toxic or otherwise undesirable substances from the bloodstream or a body tissue.…”

Section: Requirements For the Use Of Enzymes In Therapymentioning

confidence: 99%

Enzymes, 6. Enzymes in Therapy

Kresse

2008

Ullmann's Encyclopedia of Industrial Chemistry

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The article contains sections titled: 1. Requirements for the Use of Enzymes in Therapy 2. Coping with Peculiar Protein Properties 3. Sources of Enzymes and Production Systems 4. Overview of Therapeutic Enzymes

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“…The asparaginase activity was assayed by measuring the appearance of aspartic acid produced enzymatically from the hydrolysis of asparagine (asn) (reaction [2]). asparaginase asparagine + H2O…”

Section: Synthesis Of the Ap-peg-bsa Hydrogelsmentioning

confidence: 99%

Bioapplication of Poly(ethylene glycol)—Albumin Hydrogels: Matrix for Enzyme Immobilization

D'Urso

Jean-François

Fortier

1996

ACS Symposium Series

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The feasibility of enzyme immobilization into PEG-albumin hydrogels is demonstrated with acid phosphatase and asparaginase. After immobilization, the apparent Km of acid phosphatase was increased by about 11 times when compared with the soluble enzyme. To the contrary, the apparent Km of asparaginase dramatically increased by 150 times, after immobilization. A denaturing effect of the hydrogel swelling on the enzyme activity is hypothesised. For both enzymes, the operational stability at 37 °C was markedly improved by immobilization into the PEG-albumin hydrogels. The in vitro biodegradability of these hydrogels is also demonstrated through protease digestion.Enzymes have long been used in the biomedical field as diagnostic tools or disease markers (1). In the last decade however, they have found new applications as therapeutic agents (2-3). The therapeutic action of an enzyme consists mainly in degrading a substance which is toxic for the organism, due to a pathologic accumulation, an inherited deficiency or an intoxication. A list of enzymes already involved or potentially useful in therapy has been published recently (4). There are various domains of application such as replacement therapy (5-6), spécifie detoxification, scavengers (7), antineoplastic agents (8-11), and prevention of clot formation. Even though there were numerous possibilities of enzyme therapy, two major problems were rapidly identified

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Enzymes as agents for the treatment of disease

Cited by 29 publications

References 106 publications

Plasma Neuromediators and The Enzymatic Profile of Sheep

Plasma Neuromediators and The Enzymatic Profile of Sheep

Enzymes, 6. Enzymes in Therapy

Bioapplication of Poly(ethylene glycol)—Albumin Hydrogels: Matrix for Enzyme Immobilization

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