Enzyme variation inChthamalus stellatusandChthamalus montagui(Crustacea: Cirripedia): evidence for the presence ofC. montaguiin the Adriatic

Adult barnacles of Chthamalus stellatus and Chthamalus montaguj were collected in August 1998, from Garrettstown Co. Cork and Kdkee Co. Clare, In southwest and west Ireland, respectively Attached cypris larvae were collected during the settlement season of C. stellatus and C. montagui, on 2 shores in Co. Cork and 1 shore on Clare Island. Co. Mayo in west Ireland. Cypnd collections were made during either August or September, in 1992, 1994 and 1998 and all cyprids were measured along their carapace length. Size-frequency histograms were produced for each sampling occasion Total DNA was extracted and the COI-CO11 mitochondr~al genes were PCR amplified, both from selected adults and cypnds of each species collected in 1998. Composite haplotypes, produced by digesting PCR products with the restriction endonucleases Tag1 and RsaI, showed clear identification between the 2 species as adults and larvae. Of the 68 cyprids examined, those measuring 525 pm and under were found to have RFLP profiles corresponding with C. montagui adults, while those of 550 pm and larger were found to match C. stellatus adults. The findings verify previous length-frequency analys~s Carapace length is a n important character in the identification of C. stellatus and C. montagui cypnds The PCR primers developed during this study will also amplify DNA from 4 other species of acorn barnacle

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Verification of cyprid size as a tool in the identification of two European species of Chthamalus barnacles using mtDNA-RFLP analysis

Power¹,

Piyapattanakorn²,

O’Riordan³

et al. 1999

“…Subsequently, the most comprehensive distribution of these species in the northeast Atlantic, the Mediterranean, and the Black Sea was presented by Pannacciulli et al (1997). Dando et al (1979) compared the differences among populations of C. stellatus and C. montagui from the Adriatic and southwest England and found that C. montagui from the Adriatic differed from the English population, whereas C. stellatus from all sites had similar allele frequencies. Slight variations in the allozyme frequencies of the glutamate oxaloacetate transaminase (GOT) locus of C. stellatus were later reported (Pannacciulli et al 1997), suggesting genetic differences between the Atlantic and the Mediterranean populations.…”

Section: Introductionmentioning

confidence: 99%

New molecular markers for revealing the population structure of Chthamalus stellatus in the Mediterranean and the eastern Atlantic

Sasson¹,

Simon‐Blecher²,

Achituv³

2012

This study addressed the hypothesis that the lack of genetic variation among populations of the barnacle Chthamalus stellatus, detected in previous phylogeographic studies, is due to the limitations of the selected markers. We developed novel markers for this species based on 2 mitochondrial sequences, i.e. a 640 bp fragment of NADH dehydrogenase subunit 2 (ND-2) and a 228 bp fragment of the control region (CR). We found that ND-2 is highly conserved in the chthamalids and thus is not suitable for phylogenetic analyses of this group. In the CR, 204 haplotypes were recognized, of which 182 were singletons. The range of pairwise differences between populations was 6.7 to 8.9. The high variance (85 to 89%) of the CR occurs within populations, leaving only 11 to 15% of the variation between populations. The model separating the Azores population from the other Atlantic populations, and the Mediterranean population from the Atlantic populations, gave the highest variation among groups. But due to the limitations of the markers used, the hypothesis that there is genetic variation among populations of C. stellatus needs further support. The low variability of the ND-2 gene and the high variability of the CR limit the validity separation of populations of C. stellatus. The phylogeographical study of chtha malids requires development of different genetic markers; it is possible that the use of microsatellites could fulfill these requirements and reveal significant differences among populations.

“…We therefore compared Hawaiian material with several other species, both by conventional morphology and by allozyme electrophoresis. Previous investigations of Chthamalus species have shown the value of allozyme coniparisons, not only in separating subgenera and species but also in pointing out possible varietal differences (Dando et al 1979, Hedgecock 1979, Dando & Southward 1980, 1981, Miller et al 1989). The Hawaiian specimens were externally unlike typical Chthamalus species from the Pacific coast of Amer- Southern and Central California and its southern allies, including C. panaa book on the marine fauna for Hawaii), in Pearl Harbor (by S.L.C.…”

Section: Introductionmentioning

confidence: 99%

Invasion of Hawaiian shores by an Atlantic barnacle

Southward¹,

Burton²,

Coles³

et al. 1998

A largely vacant niche in the upper mid-littoral zone of sheltered Hawaiian shores is now occupied by Chtharnalusproteus Dando & Southward, 1980 (Crustacea, Cirripedia), otherwise found in the Caribbean, the Gulf of Mexico and Brazil. The identity of the species was established by morphological details and confirmed by allozyrne electrophoresis. Previously, chthamaline barnacles have rarely been found fouling hulls of ships; this is the first recorded instance of regular occurrence on ships as well as of remote dispersal in the genus. While the introduction of C. proteus apparently has had little impact so far, there is a need to step up monitoring programs so as to prevent rather than simply detect future introductions.