Two experiments were conducted to evaluate the performance of Leghorn hens fed two barley cultivars, Morex and Glenn (Experiment 1), and a high protein (Experiment 2) feed grade barley (HPB). In Experiment 1 isonitrogenous and isocaloric barley diets were formulated to replace 17, 33, 50, 67, 83, and 100% of corn and fed to hens from 20 through 36 wk of age. Egg production and egg weights from hens fed barley diets were similar and equivalent to those of hens on the corn diet. Values for feed utilization (FU) in grams of feed per gram egg mass for Morex barley, Glenn barley, and corn diets were 2.68, 2.64, and 2.36 and for feed consumption in grams of feed per day were 116.48,115.26, and 106.23, respectively. In Experiment 2 the HPB diets were formulated to replace all corn with equivalent protein content (15% CP) and differences in ME content, lysine, and methionine levels. The HPB-soybean control diet (HPB-SBM) contained 6% tallow and 2,605 kcal ME/kg diet. These diets were fed to layers from 36 through 64 wk of age. Egg weights were significantly (P<.05) reduced by at least 1 g when dietary lysine was decreased from .72 to .68%, dietary methionine decreased from .32 to .29%, and ME decreased from 2,605 to 2,310 kcal ME/kg diet. Decreased egg weights from hens fed test barley diets did not significantly increase shell weight per unit surface area. The FU for the HPB-SBM diet containing added tallow was 2.38 compared with a value of 2.68 for the HPB diet without tallow. The reduction of dietary intakes of ME, lysine, and methionine significantly reduced (P<.05) body weight gains of layers from 36 to 64 wk of age. (