Enzyme responsive polymer hydrogel beads

Thornton, Paul D.; McConnell, Gail; Ulijn, Rein V.

doi:10.1039/b511005j

Cited by 114 publications

(77 citation statements)

References 24 publications

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“…Similarly, proteolytically degradable PEG hydrogel networks can also be used to incorporate and release growth factor proteins [49]. It has been recently demonstrated that a series of enzyme-cleavable linker-containing hydrogel beads can specifically respond to target enzymes for the controlled release of growth factors [166]. Hydrogel formation can also be triggered by enzyme-catalysed reactions.…”

Section: Growth Factor Release On Demandmentioning

confidence: 99%

Growth factor delivery-based tissue engineering: general approaches and a review of recent developments

Lee

Silva

Mooney

2010

J. R. Soc. Interface.

1,188

952

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The identification and production of recombinant morphogens and growth factors that play key roles in tissue regeneration have generated much enthusiasm and numerous clinical trials, but the results of many of these trials have been largely disappointing. Interestingly, the trials that have shown benefit all contain a common denominator, the presence of a material carrier, suggesting strongly that spatio-temporal control over the location and bioactivity of factors after introduction into the body is crucial to achieve tangible therapeutic effect. Sophisticated materials systems that regulate the biological presentation of growth factors represent an attractive new generation of therapeutic agents for the treatment of a wide variety of diseases. This review provides an overview of growth factor delivery in tissue engineering. Certain fundamental issues and design strategies relevant to the material carriers that are being actively pursued to address specific technical objectives are discussed. Recent progress highlights the importance of materials science and engineering in growth factor delivery approaches to regenerative medicine.

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Section: Growth Factor Release On Demandmentioning

confidence: 99%

Growth factor delivery-based tissue engineering: general approaches and a review of recent developments

Lee

Silva

Mooney

2010

J. R. Soc. Interface.

1,188

952

View full text Add to dashboard Cite

show abstract

“…[62][63][64][65][66][67][68][69][70][71] However, the structural changes of protein-responsive hydrogels in the literature were based on the degradation of polymer networks caused by an enzymatic reaction. Studies on protein-responsive hydrogels that undergo reversible swelling/shrinking in response to a target protein have not been undertaken in spite of their potential applications as smart biomaterials.…”

Section: Antigen-responsive Hydrogelsmentioning

confidence: 99%

Preparation of smart soft materials using molecular complexes

Miyata

2010

Polym J

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This article provides a short overview of our research regarding the preparation of smart soft materials using molecular complexes that reversibly associate and dissociate in response to environmental changes. Stimuli-responsive hydrogels that show swelling/shrinking in response to pH and temperature were prepared by copolymerization of a monomer bearing a phosphate group and other various monomers. Hydrogels with phosphate groups were useful tools for the construction of self-regulated drug delivery systems. Furthermore, we proposed a strategy for the preparation of biomolecule-responsive hydrogels that use reversible crosslinks in the networks of biomolecular complexes. Based on this strategy, we have prepared two types of biomolecule-responsive hydrogel that undergo changes in volume in response to target biomolecules. This was accomplished using biomolecular complexes such as antigen-antibody complexes and saccharide-lectin complexes and both a biomolecule-crosslinked hydrogel and a biomolecule-imprinted hydrogel have been synthesized with this approach. Biomolecule-crosslinked hydrogels, such as glucose-and antigen-responsive hydrogels, swelled in the presence of a target biomolecule due to the dissociation of biomolecular complexes that act as reversible crosslinks. On the other hand, biomolecule-imprinted hydrogels, such as tumor marker glycoprotein-responsive hydrogels, shrank in response to a target biomolecule due to the formation of a complex between ligands (lectin and antibody) and the target biomolecule. Thus, biomolecule-responsive hydrogels have many potential applications as smart biomaterials in biomedical fields. Although most smart soft materials prepared using molecular complexes still require further research, they are likely to become important materials in the future.

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“…Thus, the enzyme-responsive hydrogels are typically made out of enzyme-sensitive substrates and another joint component able to direct or control specific interactions leading to macroscopic transitions. The enzyme catalytic action on the substrate leads to the swelling or collapse of the respective gels and/or the transformation of its surface properties [2,33,34]. This enzyme responsive class includes enzyme-degradable hydrogels: albumine and chitosan hydrogels N-acetyl-Dglucosamine, among others.…”

Section: Enzyme Responsive Polymersmentioning

confidence: 99%

“…[2,33,34] Enzyme catalytic action on the substrate lead to gels swelling/collapse, or the transformation of surface properties Proteases-responsive hydrogels: typically the preformed network is exposed to a protease enzyme, and hydrolysis of protein or peptide based crosslinkers in the network leads to gel degradation and subsequent release of encapsulated contents. …”

Section: Enzyme-responsivementioning

confidence: 99%

See 1 more Smart Citation

Responsive Polymers for Crop Protection

et al. 2010

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This review outlines the responsive polymer methods currently in use with their potential application to plant protection and puts forward plant-specific mechanisms as stimuli in newly devised methods for smart release of crop protection agents (CPAs). CPAs include chemicals (fungicides, insecticides, herbicides), biochemicals (antibiotics, RNAbased vaccines for plant viruses), semiochemicals (pheromones, repellents, allomones), microbial pesticides, growth regulators (insect and plant) or micronutrients, all with crop protection effects. This appraisal focuses on emerging uses of polymer nano-encapsulated CPAs. Firstly, the most interesting advances in controlled release methods are critically discussed with their advantages and drawbacks. Secondly, several plant-specific stimulibased smart methods are anticipated for use alongside the polymer nano-or microcapsules. These new CPA release methods are designed to (i) protect plants against infection produced by fungi or bacteria, and (ii) apply micro-nutrients when the plants need it the most. Thus, we foresee (i) the responsive release of nano-encapsulated bioinsecticides regulated by plant stress enzymes, and (ii) the delivery of micro-nutrients synchronized by the nature or intensity of plant root exudates. Such continued advances of nano-scale smart polymer-based CPAs for the protection of crops herald a "small revolution" for the benefit of sustainable agriculture.Keywords: responsive polymers; crop protection agent (CPA); CPA nanocapsule; nanoscience; nanotechnology; plant-specific stimuli; intelligent self-regulated release OPEN ACCESSPolymers 2010, 2 230 Glossary of TermsAllomone: a pheromone that induces a behavioral or physiological change in a member of another species that is of benefit to the producer. Bio-insecticide: the natural substance made and used for eliminating plant-eating insects. Cuticle: the layer of cutin (waxy waterproof substance, consisting of derivatives of fatty acids) covering the epidermis of the aerial parts of plants. Elicitor: a substance that induces the defense mechanisms in higher plants. Epidermis: the outer protective layer of plant cells. Parasitic plant: a plant that derives some or all of its sustenance from another plant. Perennial plant: a plant that lives for more than two years. Phloem: a plant tissue that conducts organic nutrients substances to all parts of the plant. Phytopathogen: an organism that causes a disease in a plant. RNA-based vaccines for plant viruses: Double-stranded RNA (dsRNA) is a RNA with two complementary strands, which is converted to small interfering (siRNA), involved in the RNA interference (RNAi) pathway, including plant viruses RNA silencing. Root exudate: any substance released through the pores of a diseased or injured plant root.

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Enzyme responsive polymer hydrogel beads

Abstract: We report on a new class of enzyme responsive polymer hydrogels, the molecular accessibility of which can be changed selectively by enzymes present in a sample fluid.

Cited by 114 publications

References 24 publications

Growth factor delivery-based tissue engineering: general approaches and a review of recent developments

Growth factor delivery-based tissue engineering: general approaches and a review of recent developments

Preparation of smart soft materials using molecular complexes

Responsive Polymers for Crop Protection

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