Enzyme-responsive mechanism based on multi-walled carbon nanotubes and pectin complex tablets for oral colon-specific drug delivery system

Zhu, Wenquan; Han, Chun; Dong, Yan; Jian, Baiyu

doi:10.1007/s10967-019-06501-0

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…The optimization of pectinase was carried out by Taguchi orthogonal array method. The maximal pectinase production from Streptomyces fumigatiscleroticus VIT-SP4 was observed at pH 6 which is similar to earlier study ( Zhu et al, 2019 ) and it was comparable to pectinase production from Streptomyces erumpen s MTCC 7317 at pH 7 ( Kar and Ray, 2011 ). The optimal temperature for the maximum pectinase production by the potential isolate VIT-SP4 was found to be 35 °C which is the same compared to the soil temperature of dumpsites which was about 35 to 40 °C as reported.…”

Section: Discussionsupporting

confidence: 89%

“…This model is predominantly used for optimizing the components of media required for microbial derived pectinase and it has been immensely used for the optimization of biochemical and chemical reactions like enzyme yield ( Barbosa et al, 2010 ), optimization of media ( Kunamneni and Singh, 2005 ), constraints for enzyme hydrolysis ( Shieh and Lai, 2000 ), setting up of criteria for food processing ( Ozer et al, 2004 ). Pectinase enzyme has been reported for the drug-release studies for colon treatment by the degradation of pectin-coated tablets and it also used for pulsatile drug delivery studies ( Zhu et al, 2019 , Liu et al, 2012 ). In recent scenario, there is an interest in the microbial self-healing process in case of crack repair of concrete due to its advantage like long-lasting, eco-friendly and efficiency ( Wang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach

Govindaraji

Vuppu

2020

Saudi Journal of Biological Sciences

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Pectinases are enzymes which are widely distributed in microbes that are present in pectin enriched sites. The agro-industrial residues can be utilized in the industrial scale for low-cost and efficient pectinase production in an eco-friendly approach. This study employs low-cost substrates (i.e. culinary fruit peels) for maximum pectinase production from novel Streptomyces fumigatiscleroticus VIT-SP4. The extraction and characterization of pectin from different fruit peels were investigated and pectinase activity was analyzed. The orange pectin gave maximum pectinase activity of about 45.93 (U/mL). Further, statistical optimization of process parameters was studied by using Taguchi method showed optimum values of pH-6, temperature −35 °C, orange pectin% − 2.5, incubation time- 48 h and RPM- 200 rpm and pectinase activity was found to be 98.65 (U/mL). The response surface methodology (RSM) was used for the optimization of media components which revealed that starch −1.17%, yeast extract-2%, and orange pectin% − 0.75% produces maximum pectinase of about 170.05 (U/mL). The drug-delivery study showed drug release was not observed at initial pH 3 after 4 h. The immediate drug release was noted at pH 6 caused due to disintegration of pectin by the pectinase activity. The self-healing of cracks by spray culture technique was investigated. The crack healing was observed up to 0.50 mm wide after 12 days. This confirms the ability of actinomycete spores to survive and they react to form calcite complex directly helps in crack healing process. This low-cost microbial pectinase can be used in drug delivery and concrete crack-healing applications sectors in future.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach

Govindaraji

Vuppu

2020

Saudi Journal of Biological Sciences

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“…According to these physiological characteristics, traditional drug delivery systems based on colonic physiological characteristics, such as pH-dependent 17 , time-dependent 18 , and pressure-controlled 19 , have been proposed. In addition, passive targeting drug delivery systems initiated by microbial flora, such as enzyme-triggered 20 , prodrug delivery systems 21 , enzyme-degradable polymer-coated drug delivery systems 22 , and complex colon targeting drug delivery systems 23 , which combine several delivery systems, are used. In the field of drug carrier materials, chemical modification of carrier materials or carrier materials with strong sensitivity and specificity, stable drug release process, and nontoxic side effects is currently a research hotspot in this field.…”

Section: Advantages and Development Of Oral Colon-targeted Drug Delivmentioning

confidence: 99%

Design and application of oral colon administration system

Cheng

Huang

2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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Oral colon administration system has become a new method to treat intestinal diseases. The implementation of colon drug delivery system is restricted by many aspects, including physical and chemical properties, drug delivery mode, gastrointestinal physiological factors, and so on. Delivery methods to overcome these challenges revolve around the mechanisms of drug delivery, including the use of rational dosage forms to avoid the complex pH environment, and the prevention of drug release and absorption in the upper digestive tract.

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“…[28,31] Pectinase It causes the degradation of pectin chains, a polysaccharide found in the cell wall of plants. [32,33] Peptidase It plays a role in protein digestion before absorption. [34] Lipase It is produced in the pancreas and oversees fat digestion.…”

Section: Trypsinmentioning

confidence: 99%

Novel Developments on Stimuli-Responsive Probiotic Encapsulates: From Smart Hydrogels to Nanostructured Platforms

et al. 2022

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Biomaterials engineering and biotechnology have advanced significantly towards probiotic encapsulation with encouraging results in assuring sufficient bioactivity. However, some major challenges remain to be addressed, and these include maintaining stability in different compartments of the gastrointestinal tract (GIT), favoring adhesion only at the site of action, and increasing residence times. An alternative to addressing such challenges is to manufacture encapsulates with stimuli-responsive polymers, such that controlled release is achievable by incorporating moieties that respond to chemical and physical stimuli present along the GIT. This review highlights, therefore, such emerging delivery matrices going from a comprehensive description of addressable stimuli in each GIT compartment to novel synthesis and functionalization techniques to currently employed materials used for probiotic’s encapsulation and achieving multi-modal delivery and multi-stimuli responses. Next, we explored the routes for encapsulates design to enhance their performance in terms of degradation kinetics, adsorption, and mucus and gut microbiome interactions. Finally, we present the clinical perspectives of implementing novel probiotics and the challenges to assure scalability and cost-effectiveness, prerequisites for an eventual niche market penetration.

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Enzyme-responsive mechanism based on multi-walled carbon nanotubes and pectin complex tablets for oral colon-specific drug delivery system

Cited by 14 publications

References 33 publications

Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach

Characterisation of pectin and optimization of pectinase enzyme from novel Streptomyces fumigatiscleroticus VIT-SP4 for drug delivery and concrete crack-healing applications: An eco-friendly approach

Design and application of oral colon administration system

Novel Developments on Stimuli-Responsive Probiotic Encapsulates: From Smart Hydrogels to Nanostructured Platforms

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