1999
DOI: 10.1038/5250
|View full text |Cite
|
Sign up to set email alerts
|

Enzyme modification by polymers with solublities that change in response to photoirradiation in organic media

Abstract: We have synthesized a hybrid subtilisin the solubility of which can be regulated by photoirradiation through coupling with a photoresponsive copolymer that carries spiropyran groups in its side chains. The copolymer was synthesized by polymerization of methacrylate, methacrylic acid, and spiropyran-carrying methacrylate. It was then covalently bonded to the amino groups of subtilisin Carlsberg via its carboxyl groups using a carbodiimide coupling agent. The hybrid subtilisin was perfectly soluble in toluene an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

0
30
0

Year Published

1999
1999
2014
2014

Publication Types

Select...
7
1
1

Relationship

4
5

Authors

Journals

citations
Cited by 62 publications
(30 citation statements)
references
References 26 publications
(19 reference statements)
0
30
0
Order By: Relevance
“…Through light irradiation, the amphiphilicity change of spiropyran was employed to tune the solubility of the enzyme in organic solvent. [94] Recently, a light-induced nanovalve derived from one channel protein was reported. By embedding spiropyran groups in a channel protein, Escherichia coli, a 3-nm pore could be opened and closed reversibly by the photochromatism of spiropyran.…”
Section: Photocontrolled Methodsmentioning
confidence: 99%
“…Through light irradiation, the amphiphilicity change of spiropyran was employed to tune the solubility of the enzyme in organic solvent. [94] Recently, a light-induced nanovalve derived from one channel protein was reported. By embedding spiropyran groups in a channel protein, Escherichia coli, a 3-nm pore could be opened and closed reversibly by the photochromatism of spiropyran.…”
Section: Photocontrolled Methodsmentioning
confidence: 99%
“…We can now prepare many types of complex macromolecules, including new synthetic polymer–biomacromolecular conjugates and biologically processed biomacromolecules. The former types include the conjugation of biological macromolecules with polyethylene glycol or stimuli-responsive polymers for medical or bioindustrial applications [7177]. The latter molecules are biological modifications of DNA, RNA, or polypeptides.…”
Section: Designs Based On Peptide Ligationmentioning
confidence: 99%
“…Ito and colleagues modified the enzymes with other polymers and extended the usefulness of the modified enzymes (1,2,(17)(18)(19).…”
Section: Introductionmentioning
confidence: 97%