Enzyme modification by polymers with solublities that change in response to photoirradiation in organic media

Ito, Yoshihiro; Sugimura, Norio; Kwon, Oh Hyeong; Imanishi, Yukio

doi:10.1038/5250

Cited by 62 publications

(30 citation statements)

References 26 publications

(19 reference statements)

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“…Through light irradiation, the amphiphilicity change of spiropyran was employed to tune the solubility of the enzyme in organic solvent. [94] Recently, a light-induced nanovalve derived from one channel protein was reported. By embedding spiropyran groups in a channel protein, Escherichia coli, a 3-nm pore could be opened and closed reversibly by the photochromatism of spiropyran.…”

Section: Photocontrolled Methodsmentioning

confidence: 99%

Tuning the Amphiphilicity of Building Blocks: Controlled Self‐Assembly and Disassembly for Functional Supramolecular Materials

2009

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Amphiphilicity is one of the molecular bases for self‐assembly. By tuning the amphiphilicity of building blocks, controllable self‐assembly can be realized. This article reviews different routes for tuning amphiphilicity and discusses different possibilities for self‐assembly and disassembly in a controlled manner. In general, this includes irreversible and reversible routes. The irreversible routes concern irreversible reactions taking place on the building blocks and changing their molecular amphiphilicity. The building blocks are then able to self‐assemble to form different supramolecular structures, but cannot remain stable upon loss of amphiphilicity. Compared to the irreversible routes, the reversible routes are more attractive due to the good control over the assembly and disassembly of the supramolecular structure formed via tuning of the amphiphilicity. These routes involve reversible chemical reactions and supramolecular approaches, and different external stimuli can be used to trigger reversible changes of amphiphilicity, including light, redox, pH, and enzymes. It is anticipated that this line of research can lead to the fabrication of new functional supramolecular assemblies and materials.

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Section: Photocontrolled Methodsmentioning

confidence: 99%

Tuning the Amphiphilicity of Building Blocks: Controlled Self‐Assembly and Disassembly for Functional Supramolecular Materials

2009

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“…We can now prepare many types of complex macromolecules, including new synthetic polymer–biomacromolecular conjugates and biologically processed biomacromolecules. The former types include the conjugation of biological macromolecules with polyethylene glycol or stimuli-responsive polymers for medical or bioindustrial applications [71–77]. The latter molecules are biological modifications of DNA, RNA, or polypeptides.…”

Section: Designs Based On Peptide Ligationmentioning

confidence: 99%

Design and Synthesis of Binding Growth Factors

Tada

Kitajima

Ito

2012

IJMS

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Growth factors play important roles in tissue regeneration. However, because of their instability and diffusible nature, improvements in their performance would be desirable for therapeutic applications. Conferring binding affinities would be one way to improve their applicability. Here we review techniques for conjugating growth factors to polypeptides with particular affinities. Conjugation has been designed at the level of gene fusion and of polypeptide ligation. We summarize and discuss the designs and applications of binding growth factors prepared by such conjugation approaches.

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“…Ito and colleagues modified the enzymes with other polymers and extended the usefulness of the modified enzymes (1,2,(17)(18)(19).…”

Section: Introductionmentioning

confidence: 97%

PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation

Tada

Abe

Ito

2013

ACS Symposium Series

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Polyethylene glycol (PEG) is an amphiphilic polymer that is soluble in both water and organic media and can add many functions to biopolymers by conjugation. Here, PEG was coupled to antibodies or oligonucleotides for solubilization into organic media. The former interacted with antigens and the latter formed specific structures and had catalytic activity in organic media. In addition, we attempted to incorporate PEG genetically into a polypeptide. Although the PEG incorporation ratio decreased as the molecular weight of PEG was increased, PEG with a molecular weight of ≤1000 Da was incorporated successfully. The potential applications of PEGylated biopolymers and the methodology for gene PEGylation are discussed.

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Enzyme modification by polymers with solublities that change in response to photoirradiation in organic media

Cited by 62 publications

References 26 publications

Tuning the Amphiphilicity of Building Blocks: Controlled Self‐Assembly and Disassembly for Functional Supramolecular Materials

Tuning the Amphiphilicity of Building Blocks: Controlled Self‐Assembly and Disassembly for Functional Supramolecular Materials

Design and Synthesis of Binding Growth Factors

PEGylated Antibodies and DNA in Organic Media and Genetic PEGylation

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