Enzyme-Mediated Solvent Extraction of Carotenoids from Marigold Flower (<i>Tagetes erecta</i>)

Bárzana, Eduardo; Rubio, D. F. M.; Santamaría, Rosa I.; Garcia-Correa, O; García, Francisco Javier Espinosa; Sanz, V E Ridaura; López-Munguı́a, Agustı́n

doi:10.1021/jf025550q

Cited by 124 publications

(70 citation statements)

References 13 publications

(19 reference statements)

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“…This increment can be explained in terms of the hydrolytic capacity of enzymes to degrade the main components of the cell wall, releasing sugars from macro-molecules like cellulose, hemicellulose and pectin. A similar behavior has been referenced previously by Barzana et al (2002) for the carotenoids extraction from marigold flower flowers.…”

Section: Enzymatically-aided Antioxidant Extraction Processsupporting

confidence: 80%

Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

Laroze

Soto

Zúñiga

2010

Electron Journ of Biotechnol

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The effect of enzymatic hydrolysis on phenolic antioxidant extraction was studied in raspberry solid wastes. This by-product possesses high content of crude fiber (60%) and low values of protein, oil and ash. Raspberry fiber composition suggests that biocatalysts with cellulase, hemicellulase and pectinase activities would be useful for carrying out an enzymatically assisted antioxidant extraction. Hydro-alcoholic extraction was done using different commercial enzymes. Total phenol content and antioxidant activity of enzyme-hydrolyzed residue extracts were measured and compared with those obtained without enzyme application.All biocatalysts evaluated increased soluble solids in comparison to the non-enzymatic control. Among them, Grindamyl and Maxoliva offered the best recovery of polyphenols. Enzymatic assisted extraction with an hydro-ethanolic mixture (75:25, v/v) during 18 hrs at 50°C increased phenolic content up to 35% and antioxidant capacity around 50%, 15% and 30% according to 2,2-diphenyl-1-picrylhydrazyl = 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl) (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods, respectively. Use of a higher enzyme concentration significantly improved extraction of phenolic antioxidants.

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Section: Enzymatically-aided Antioxidant Extraction Processsupporting

confidence: 80%

Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

Laroze

Soto

Zúñiga

2010

Electron Journ of Biotechnol

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“…Of these solvents, lutein is most soluble to dichloromethane and acetone and, to a lesser extent, to hexanes and alcohols (Craft and Soares 1992) where free lutein is concerned. Barzana et al (2002) showed that free lutein is less prone to isomerisation and degradation in hexane, which might be a benefit, although the solubility is lower. It has to be noted, that the solubility of free lutein differs immensely from the solubility of lutein esters in some solvent systems (Amar et al 2003).…”

Section: Lutein Oil Extraction Emulsion Stability Polysaccharidesmentioning

confidence: 99%

“…Such benefits can be obtained using careful grinding but also enzyme-assisted extraction. Barzana et al (2002) tested some combinations of plant cell wall degrading enzymes and hexane extraction, and they reported extraction efficiencies of up to 97 percent.…”

Section: Lutein Oil Extraction Emulsion Stability Polysaccharidesmentioning

confidence: 99%

Enzyme-Assisted Oil Extraction of Lutein from Marigold (Tagetes erecta) Flowers and Stability of Lutein during Storage

Kaimainen¹,

Järvenpää²,

Huopalahti³

2015

International Journal of Agricultural and Food Research

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Marigold (Tagetes erecta) flowers are a rich source of organic solvents. In this study, lutein was extracted from marigold flowers with enzyme extraction without using organic solvents. Extraction produced oil that contained 0.36 mg/ml lutein (present as lutein esters but calculated as free lutein), and the yield was comparable to solvent extraction. The oil containing lutein was used to produce oil mixtures as stabilizers and some emulsions were also s containing lutein were stored in the dark at 20 monitored during storage. Stability of lutein was good in oil (85 emulsions (77-91%) but slightly worse in spray extraction of lutein from marigold flowers is a potential alternative to solvent extraction with comparable efficiency. In addition, there are no solvent residues in showed good stability of lutein in oil or in emulsions stored in the dark at room temperature, and these preparations could be used in different food products.

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“…Accordingly, more pigment (anthocyanin) was extracted during the vinification process, when enzymes were applied on grape skin (PiemoliniBarreto et al, 2014). In another example, the extraction of carotenoids from marigold flower or tomato skin (Barzana et al, 2002;Dehghan-Shoar et al, 2011) indicates that enzymes can also be useful for the extraction of bioactive compounds from other plant sources.…”

Section: Parametersmentioning

confidence: 99%

Application of enzymatic preparations to produce araçá pulp and juice

Sandri¹,

Piemolini-Barreto²,

Fontana³

et al. 2014

Food Sci. Technol (Campinas)

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In the present study, the effect of pectinases on the production of pulp and juice of araçá and the presence of bioactive compounds were evaluated. An enzyme extract (EE) produced by Aspergillus niger LB-02-SF in solid state fermentation and the commercial enzyme Ultrazym  AFP-L were used in this study. After enzyme treatment with the EE preparation, the extraction yield increased by 23.1% and viscosity decreased by 42.8%, during pulp maceration. During juice processing, there was an increase of 70.6% in clarification and a decrease of 72.87% in turbidity. Higher values of these parameters, 47.7, 69.0, 80.7, and 79.7%, respectively, were obtained using the Ultrazym  AFP-L, which also led to a significant increase in the polyphenol content, both in the pulp (24%) and in the juice (28%), with a less pronounced effect when the EE was applied (10 and 21%, respectively). The anthocyanins content in the araçá pulp increased after treatment with the commercial preparation (23%), and there was no significant increase with the use of EE. The use of Ultrazym  AFP-L increased the b-carotene content by 29.4% in the fruit pulp, while the treatment with EE did not result in significant changes compared with those of the juice and pulp controls.

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Enzyme-Mediated Solvent Extraction of Carotenoids from Marigold Flower (Tagetes erecta)

Cited by 124 publications

References 13 publications

Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

Phenolic antioxidants extraction from raspberry wastes assisted by-enzymes

Enzyme-Assisted Oil Extraction of Lutein from Marigold (Tagetes erecta) Flowers and Stability of Lutein during Storage

Application of enzymatic preparations to produce araçá pulp and juice

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