Enzyme mass transfer coefficient in aqueous two phase system using a packed extraction column

Abstract

Fractional dispersed phase hold‐up and dispersed side mass transfer coefficients for amyloglucosidase were measured in a 56 mm i.d. packed extraction column using a sodium sulphate‐polyethylene glycol 4000 system. Raschig rings (3 to 13.3 mm), Berl saddles (12 mm), Pall rings (12.6 mm), glass spheres (5.2 mm) and structured wire gauze were used as packings. The effect of packing size was investigated in the case of ceramic Raschig rings. The effect of phase composition of the aqueous phase system also was studied. Correlations have been developed for fractional dispersed phase hold‐up and volumetric mass transfer coefficient with packing voidage, dry surface area of packings, superficial dispersed phase velocity and the liquid phase physical properties.

Mass transfer in aqueous two-phases system packed column

“…The K D a obtained in the continuous process at 0.11 mm/s superficial dispersed phase velocity using 6 mm glass Raschig rings (0.1 min −1 ) is 10 times higher than with the semi-batch operation (0.018 min −1 ) using 6.7 ceramic Raschig rings [2]. The counter-current process causes a higher turbulence inside the equipment favoring the mass transfer phenomena.…”

“…Due to these benefits Patil et al [2] adapted, with success, a packed column for a semi-continuous protein extraction unit using ATPS.…”

Extração de biomoleculas em sistemas de duas fases aquosas convencionais e com polimeros termossensiveis

“…para a extração da a-amiloglicosidase (AMG)(PATIL et al, 1991). Foram avaliados os efeitos de diversos tipos de recheio (anéis de Raschig, selas de Berl, anéis de Pall, esferas de vidro e recheios estruturados) e a composição das fases sobre o coeficiente volumétrico de transferência de massa e hold-up.…”