Enzyme Markers for Somatic Embryogenesis in Vitis

Martinelli, L.; Scienza, A.; Villa, P.; Ponti, Paola De; Gianazza, Elisabetta

doi:10.1016/s0176-1617(11)80197-5

Cited by 12 publications

(5 citation statements)

References 38 publications

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“…ADH3 is involved in regulation of the effects of nitric oxide in the cell and is therefore important for the maintenance of cellular homeostasis [ 66 ]. Additionally, ADH3 has been reported to represent a class of enzyme that is closely related to the developmental stages of Vitis rupestris somatic embryos [ 67 ]. The other exception was cinnamoyl alcohol dehydrogenase (spot 333), which was unique to the control treatment.…”

Section: Resultsmentioning

confidence: 99%

“…We observed that the synthesis of certain proteins induced by PEG treatment, such as enolase, esterase and ADH3, could play an important role in the maturation of somatic papaya embryos. These proteins have been found to be associated with somatic embryo development in other species [ 60 , 61 , 67 , 106 ], and esterases are considered important biomarkers of somatic embryos in plants [ 106 ]. Additionally, the present study provides a list of PEG-induced proteins that are potential biomarker candidates for future investigations of somatic embryogenic development in papaya.…”

Section: Discussionmentioning

confidence: 99%

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Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

et al. 2014

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BackgroundSomatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and understanding of different biological responses. In addition, the identification of molecular markers could lead to the optimization of protocols allowing the use of biotechnology for papaya propagation and reproduction. This work aimed to investigate the effects of polyethylene glycol (PEG) on somatic embryo development and the protein expression profile during somatic embryo maturation in papaya (Carica papaya L.).ResultsThe maturation treatment supplemented with 6% PEG (PEG6) resulted in the greatest number of somatic embryos and induced differential protein expression compared with cultures grown under the control treatment. Among 135 spots selected for MS/MS analysis, 76 spots were successfully identified, 38 of which were common to both treatments, while 14 spots were unique to the control treatment, and 24 spots were unique to the PEG6 treatment. The identified proteins were assigned to seven categories or were unclassified. The most representative class of proteins observed in the control treatment was associated with the stress response (25.8%), while those under PEG6 treatment were carbohydrate and energy metabolism (18.4%) and the stress response (18.4%).ConclusionsThe differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

et al. 2014

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“…Somatic embryogenesis in grapevine is hindered by low efficiency and a strong genotype effect. Only a few molecular studies of the developmental steps of morphogenesis have been conducted in this plant (Gianazza et al 1992, Martinelli et al 1993). A better understanding of the factors involved in somatic embryogenesis would be required for determining central aspects of grapevine biology and for improving this plant using a biotechnological approach (Martinelli and Gribaudo 2001).…”

Section: Introductionmentioning

confidence: 99%

Polyamines and somatic embryogenesis in two Vitis vinifera cultivars

Bertoldi

Tassoni

Martinelli³

et al. 2004

Physiologia Plantarum

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Polyamine content and activities of enzymes of polyamine biosynthesis were assayed during somatic embryogenesis in Vitis vinifera callus cultures of Chardonnay and Brachetto ‘a grappolo lungo’ (Brachetto g.l.) cultivars. The analyses were carried out on embryogenic callus samples, embryos at different stages and developing plants. Polyamine content, both in the free and PCA‐soluble conjugated form, was higher in Brachetto g.l. than in Chardonnay, and putrescine was present at higher concentrations than the other polyamines. In all samples of both cultivars, ornithine decarboxylase activity (ODC, EC 4.1.1.17) was higher than arginine decarboxylase (ADC, EC 4.1.1.19), with a maximum in developing plant roots. S‐Adenosylmethionine decarboxylase (SAMDC, EC 4.1.1.50) activity displayed a similar trend. The activities of all three enzymes were detected both in the supernatant and pellet fractions, indicating for the first time the presence of SAMDC activity in the particulate fraction. Particularly in the Chardonnay cultivar, an increase in the mRNAs expression patterns of ODC and SAMDC during morphogenesis from small embryos to plantlets was detected by northern blot, suggesting a direct correlation with enzymatic activities.

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“…Plants express many dehydrogenases, but we can highlight alcohol dehydrogenase 3 (ADH3). ADH3 represents a class of enzymes that may be related to a specific stage of embryonic development in Vitis rupestris [ 56 ], Daucus carota [ 57 ] and Bactris gasipaes Kunth [ 58 ] somatic embryos. Therefore, the function of this enzyme is important, among many other functions as the possible association of this protein with embryogenic competence for somatic embryo formation.…”

Section: Discussionmentioning

confidence: 99%

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

et al. 2015

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The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to observe somatic embryo development and to identify differentially expressed proteins in embryogenic (E) and non-embryogenic (NE) callus during maturation treatment. E and NE callus were cultured on maturation culture medium supplemented with different concentrations (0.0, 0.75, 1.5 and 2.0 g L-1) of activated charcoal (AC). Somatic embryo formation and differential protein expression were evaluated at days 0 and 21 using shotgun proteomic analyses. Treatment with 1.5 g L-1 AC resulted in higher somatic embryo maturation rates (158 somatic embryos in 14 days) in E callus but has no effect in NE callus. A total of 752 co-expressed proteins were identified through the SUCEST (The Sugarcane EST Project), including many housekeeping proteins. E callus showed 65 exclusive proteins on day 0, including dehydrogenase, desiccation-related protein, callose synthase 1 and nitric oxide synthase. After 21 days on maturation treatment, 14 exclusive proteins were identified in E callus, including catalase and secreted protein. NE callus showed 23 exclusive proteins on day 0 and 10 exclusive proteins after 21 days on maturation treatment, including many proteins related to protein degradation. The induction of maturation leads to somatic embryo development, which likely depends on the expression of specific proteins throughout the process, as seen in E callus under maturation treatment. On the other hand, some exclusive proteins can also specifically prevent of somatic embryos development, as seen in the NE callus.

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Enzyme Markers for Somatic Embryogenesis in Vitis

Cited by 12 publications

References 38 publications

Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

Polyamines and somatic embryogenesis in two Vitis vinifera cultivars

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

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