Enzyme-linked immunospot assay response to recombinant CFP-10/ESAT-6 fusion protein among patients with spinal tuberculosis: implications for diagnosis and monitoring of surgical therapy

Yuan, Kai; Wu, Xueqiong; Zhang, Qiang; Zhong, Zhaoming; Chen, Jianting

doi:10.1016/j.ijid.2013.02.027

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…After being cultured overnight, spot-forming cells (SFCs) were counted by automated ELISPOT assay reader (AID ELISPOT reader system, Autoimmune Diagnostika GmbH, Strassberg, Germany). Antigen-specific T-cell responses were considered positive when the number of SFCs from the peptide-pulsed well was greater than three times as many as those in negative control [ 21 ]. The adjusted SFCs after subtracting the average negative values were expressed as SFC/10 6 PBMCs.…”

Section: Methodsmentioning

confidence: 99%

Human papilloma virus E1-specific T cell immune response is associated with the prognosis of cervical cancer patients with squamous cell carcinoma

Fan

et al. 2018

Infect Agents Cancer

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BackgroundCervical cancer is attributable to human papilloma virus (HPV) infection in the majority cases. E1, an HPV derived-protein, plays an important role in the initiation and development of cervical cancer. Our study aims to investigate the HPV E1-specific T cell response in patients with cervical squamous cell carcinoma (CSCC).MethodsA total of 66 CSCC patients with FIGO stage IIB-IIIB and 60 healthy controls were enrolled. Enzyme-Linked ImmunoSpot (ELISPOT) assays was used to measure the HPV E1-specific T cell response in the peripheral blood of these patients before treatment. The patients were treated with chemotherapy and/or radiotherapy and followed up clinically for three years. The relationship between the T cell response, various clinical characteristics and the prognosis were studied with univariate analysis, multivariate analysis and survival curve analysis.ResultsThe frequency of HPV E1-specific T cell response in peripheral blood of cervical cancer patients was 59.09%, with mean response intensity 24.56 SFC/106 PBMCs. The frequency and intensity of HPV E1-specific T cell response in patients were higher than healthy controls(p < 0.001; p = 0.009). The intensity of HPV E1-specific T cell responses were higher in the stage IIB patients and patients with no pelvic lymph node metastasis (p = 0.038; p = 0.044). Univariate analysis showed that HPV E1 specific T cell response was associated with progression-free survival (PFS) and overall survival (OS) (PFS: p = 0.021; OS: p = 0.004). Multivariate analysis showed that HPV E1-specific T cell response was an independent prognostic factor influencing PFS and OS among all the factors included in our study (PFS: HR = 7.252, 95%CI = 1.690–31.126, p = 0.008; OS: HR = 7.499, 95%CI = 1.661–33.856, p = 0.009). The survival curves showed that the rate of PFS and OS in patients with HPV E1 specific T cell response was significantly higher than those who did not response.ConclusionsOur study demonstrated that the level of HPV E1-specific T cell response was correlated with the survival of advanced patients with CSCC. Patients who displayed no HPV E1-specific T cell response were more likely to be those with poor prognosis.

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Section: Methodsmentioning

confidence: 99%

Human papilloma virus E1-specific T cell immune response is associated with the prognosis of cervical cancer patients with squamous cell carcinoma

Fan

et al. 2018

Infect Agents Cancer

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“…In recent years, an enzyme-linked immunospot (ELISPOT) assay has been commercialised (T-SPOT.TB; Oxford Immunotec, Oxford, UK) that is based on the detection of interferon-γ released by activated T lymphocytes. The ELISPOT assay has been demonstrated to provide useful support in the diagnosis of osteoarticular TB with high sensitivity and specificity [155][156][157][158].…”

Section: Level Of Evidence:mentioning

confidence: 99%

Joint EANM/ESNR and ESCMID-endorsed consensus document for the diagnosis of spine infection (spondylodiscitis) in adults

Lazzeri

Bozzao

Cataldo

et al. 2019

Eur J Nucl Med Mol Imaging

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Purpose Diagnosis of spondylodiscitis (SD) may be challenging due to the nonspecific clinical and laboratory findings and the need to perform various diagnostic tests including serologic, imaging, and microbiological examinations. Homogeneous management of SD diagnosis through international, multidisciplinary guidance would improve the sensitivity of diagnosis and lead to better patient outcome. Methods An expert specialist team, comprising nuclear medicine physicians appointed by the European Association of Nuclear Medicine (EANM), neuroradiologists appointed by the European Society of Neuroradiology (ESNR), and infectious diseases specialists appointed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID), reviewed the literature from January 2006 to December 2015 and proposed 20 consensus statements in answer to clinical questions regarding SD diagnosis. The statements were graded by level of evidence level according to the 2011 Oxford Centre for Evidence-based Medicine criteria and included in this consensus document for the diagnosis of SD in adults. The consensus statements are the result of literature review according to PICO (P:population/patients, I:intervention/indicator, C:comparator/control, O:outcome) criteria. Evidence-based recommendations on the management of adult patients with SD, with particular attention to radiologic and nuclear medicine diagnosis, were proposed after a systematic review of the literature in the areas of nuclear medicine, radiology, infectious diseases, and microbiology. Results A diagnostic flow chart was developed based on the 20 consensus statements, scored by level of evidence according to the Oxford Centre for Evidence-based Medicine criteria. Conclusions This consensus document was developed with a final diagnostic flow chart for SD diagnosis as an aid for professionals in many fields, especially nuclear medicine physicians, radiologists, and orthopaedic and infectious diseases specialists.

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“…TDT'nin duyarlılığı %62-72 arasında değişir. Ancak bu test aktif infeksiyonu geçirilmiş infeksiyondan ayırt ettirmez ve BCG aşısı uygulananlarda da pozitif saptanır (9). Bizim hastamızda TDT anerjik olarak saptandı.…”

Section: İrdelemeunclassified

“…BCG aşısından etkilenmeyen bu testlerin vertebra tüberkülozu tanısında duyarlılık ve özgüllükleri-nin yüksek olduğu bildirilmiştir (9)(10)(11). Sunduğumuz olguda da interferon-γ salınım testi pozitif olarak saptandı.…”

Section: İrdelemeunclassified

A Case Report of Paraplegic Pott’s Disease Exhibiting Cervical Vertebral Involvement

Hekimoğlu¹,

Cesur²,

Hatipoğlu³

et al. 2017

Klimik Dergisi

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Cervical vertebral involvement is extremely rare in Pott's disease but it may lead to severe complications. Therefore, early diagnosis and treatment is required. In this report, a 23 yearold paraplegic male with cervical vertebral involvement showing magnetic resonance imaging findings for Pott's abscess was presented. Mycobacterium tuberculosis was isolated from the abscess culture and he had positive interferon-γ release assay. As this case demonstrated vertebral tuberculosis should be kept in mind in patients admitted with severe neck pain and progressive neurological deficit, and these patients should also be investigated in this respect. Klimik Dergisi 2017; 30(1): 45-7.

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Enzyme-linked immunospot assay response to recombinant CFP-10/ESAT-6 fusion protein among patients with spinal tuberculosis: implications for diagnosis and monitoring of surgical therapy

Abstract: A laboratory-developed rCFP-10/ESAT-6 ELISPOT assay is a useful adjunct to current tests for the diagnosis of spinal TB.

Cited by 6 publications

References 28 publications

Human papilloma virus E1-specific T cell immune response is associated with the prognosis of cervical cancer patients with squamous cell carcinoma

Human papilloma virus E1-specific T cell immune response is associated with the prognosis of cervical cancer patients with squamous cell carcinoma

Joint EANM/ESNR and ESCMID-endorsed consensus document for the diagnosis of spine infection (spondylodiscitis) in adults

A Case Report of Paraplegic Pott’s Disease Exhibiting Cervical Vertebral Involvement

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