Enzyme-Linked Immunosorbent Assay Kit for the Determination of Soybean Protein in Processed Foods: Interlaboratory Evaluation

Abstract

The labeling of foods containing ingredients derived from soybean is recommended in Japan because of an increasing number of patients who are allergic to soybeans. To ensure proper labeling, a novel sandwich ELISA kit for the determination of soybean protein in processed foods (FASTKIT Ver. II, Soybean, Nippon Meat Packers, Inc.; soy kit) has been developed. Five types of incurred samples (model processed foods: rice gruel, sausage, sweet adzuki bean soup, sweet potato cake, and tomato sauce) containing 10 g soybean soluble protein/g food were prepared for use in interlaboratory evaluations of the soy kit. The soy kit displayed a sufficient RSDR value (interlaboratory precision: 9.313.4 RSDR) and a high level of recovery (97114) for all the incurred samples. The RSDr value for the incurred samples was mostly <4.8. The results of this interlaboratory evaluation suggest that the soy kit can be used as a precise and reliable tool for the determination of soybean proteins in processed foods.

Detection of Soybean Proteins in Fermented Soybean Products by Using Heating Extraction

“…In previous research, we developed a reliable sandwich ELISA method with high sensitivity and specificity for detecting soybean proteins by using antibody to Gly m Bd 30K (Morishita and others ; Sakai and others ). The limit of detection of the ELISA was 1 μg/g.…”

“…Protein was extracted from food samples in accordance with a previously reported method (MHLW ; Morishita and others ; Sakai and others ). Food samples were homogenized in a food processor (IFM‐700G, Iwatani Intl.…”

“…In the previous study, we developed a reliable sandwich Enzyme Linked Immunosorbent Assay (ELISA) method with high sensitivity and specificity for detecting soybean proteins by using antibody to Gly m Bd 30K, which has been characterized as a vacuolar protein with a molecular mass of 34 kDa (Morishita and others ). The ELISA displayed sufficient repeatability and reproducibility in an interlaboratory evaluation (Sakai and others ). However, it cannot detect soybean protein in fermented soybean products (Morishita and others ).…”

Analysis To Support Allergen Risk Management: Which Way To Go?

“…When using anti-Cor a 9 antibodies in a sandwich ELISA, the robustness of the receptor-based analytical methodology was improved. 26 Similarly, antibodies against a single allergen such as the stable Gly m Bd 30K allergen were used for the detection of soybean 93,95 and antibodies against the 2S albumin for the detection of walnut in processed foods. 96 Use of Stable Peptides as Analytical Targets.…”

“…Therefore, determination of the recovery in samples incurred before the application of any processing is more accurate and should be applied in a proper validation process of any analytical method. Several studies in which evaluation of the robustness by incurring samples before processing was done are published. ,− We have shown that when determining the robustness in cookies incurred before processing was applied, only about 10% recovery were obtained . Similarly, the recovery of the DNA seems to also be affected by processing, mainly because of its degradation and/or hindered extractability …”