Enzyme-linked immunosorbent assay for detection of measles antibody

Boteler, W L; Luipersbeck, P M; Fuccillo, David A.; O'Beirne, A J

doi:10.1128/jcm.17.5.814-818.1983

Cited by 45 publications

(17 citation statements)

References 7 publications

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“…Different serological assays measure diverse subsets of MV antibodies, and it is not clear which subset reflects the cellular immune response best. Whole MV-based ELISAs detect a broad range of immunoglobulins, irrespective of their functional activities (8,15,35,50). Functional assays, such as HI and NT, are therefore considered to provide a better estimate of the immune status.…”

Section: Discussionmentioning

confidence: 99%

“…Performance of the H-ELISA. The different performance parameters of the H-ELISA were compared with those of whole-virus-based ELISAs from this study and from the literature (8,12,34). For a valid comparison, all values were recalculated with the same algorithms by adding undefined sera to the sera negative by ELISA (7).…”

Section: Detection Of Mv-specific Iggmentioning

confidence: 99%

“…Monitoring of immunity to measles relies solely on the detection of specific antibodies, but it is not clear to what extent antibody titers reflect protective cellular immunity. Past experience with enzyme-linked immunosorbent assays (ELISAs) based on whole measles virus (MV) suggests that antibodies directed against whole virus are a reliable measure of measles immunity (8,15,35,50). Assays for immunity based on recombinant proteins may offer a number of advantages over whole-virus-based ELISAs.…”

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confidence: 99%

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Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

et al. 1998

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Recombinant hemagglutinin (H) protein of the measles virus (MV) was produced in mammalian cells with a high-yield expression system based on the Semliki Forest virus replicon. Crude membrane preparations of H protein-transfected BHK-21 cells were used to coat microtiter plates to measure specific immunoglobulin G antibodies in 228 serologically defined serum samples mainly from measles late-convalescent adults. The titers by the enzyme-linked immunosorbent assay for the H protein (H-ELISA) closely correlated with neutralization test (NT) titers (R 2 = 0.66), hemagglutination inhibition test (HI) titers (R 2 = 0.64), with the titers from a certified commercial ELISA based on whole MV-infected cells (MV-ELISA;R 2 = 0.45). The correlations described above were better than those of the commercial MV-ELISA titers with the NT (R 2 = 0.52) or HI (R 2 = 0.48) titers. By using the 2nd International Standard for anti-measles serum, the detection level of the assay corresponds to 215 mIU/ml for undiluted serum, which corresponds to the estimated threshold for protective immunity. The specificity, accuracy, and positive predictive value were, in general, better for the H-ELISA than for a commercial MV-ELISA, independent of whether HI, NT, or HI and NT were used as “gold standards.” In contrast, the H-ELISA proved to be slightly less sensitive than the MV-ELISA (sensitivities, 98.6 versus 99.5%, respectively;P was not significant). The assays did not differ significantly in the number of serum samples with positive HI and NT results (n = 212) which measured false negative (H-ELISA, 2 of 212 [0.94%]; MV-ELISA, 1 of 212 [0.47%]), but the H-ELISA detected significantly more measles-susceptible individuals than the MV-ELISA (10 of 11 versus 3 of 11, respectively;P < 0.05) among the individuals whose sera had negative HI and NT results. Our data demonstrate that the H-protein preparation that we describe could be a cost-effective alternative to current whole-virus-based ELISAs for surveillance for immunity to measles and that such an assay could be more efficient in detecting susceptibility to measles. Furthermore, unlike whole MV-based antigens, H-protein would also be suitable for use in the development of a simple field test for the diagnosis of measles.

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of Mv-specific Iggmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

et al. 1998

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“…The coefficient of variation of this assay in our laboratory is 6.6%. We defined the subjects by optical density (OD) values as described by the manufacturer (Boteler et al, 1983). We classified subjects with index values ≤ 0.8 units as seronegative and classified subjects with values ≥ 1.0 units as seropositive.…”

Section: Antibody Measurementmentioning

confidence: 99%

Lack of association between transporter associated with antigen processing (TAP) and HLA‐DM gene polymorphisms and antibody levels following measles vaccination

Dhiman

Ovsyannikova

Pinsky

et al. 2003

European Journal of Immunogenetics

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The transporter associated with antigen processing (TAP) and human leukocyte antigen-DM (HLA-DM) genes are involved in the antigen-processing pathway of both HLA class I and class II-restricted antigen presentation. We hypothesized that polymorphisms within the TAP and DM genes may influence antibody levels following measles vaccination. We examined TAP and DM polymorphisms in 242 school children from Olmsted County, Minnesota, USA who received one dose of measles-mumps-rubella-II (MMR-II) vaccine at the age of 15 months. Based on the level of serum measles-specific immunoglobulin G (IgG) antibodies, subjects were classified as seronegatives (n = 72) or seropositives (n = 170). We determined TAP1 and TAP2 allele types by polymerase chain reaction (PCR) amplification of specific alleles (PASA) and determined DM allele type by PCR amplification followed by direct sequencing of the polymorphic sites. We analysed the data for any TAP or DM allelic association with antibody levels post measles vaccination using the chi-square test and univariate linear regression analysis. We found no trend in the overall distribution of TAP and DM genotype frequencies between seronegative and seropositive subjects, suggesting that TAP and DM polymorphism and antibody levels following measles vaccination are not directly associated. In addition, we did not find an association between TAP (TAP1, P = 0.71; TAP2, P = 0.87) or DM (DMA, P = 0.42; DMB, P = 0.71) homozygosity and seronegativity to measles vaccine in this study group. Our study suggests that TAP and DM gene polymorphisms do not influence antibody levels post measles vaccination.

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“…Indirect EIAs. An indirect measles EIA was performed by a modification of the method described by Boteler et al (6). Briefly, measles virus-positive antigen and negative control cells were each diluted 1:40 in deionized H20 and dried onto microtiter plates overnight.…”

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confidence: 99%

Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus

et al. 1991

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Monoclonal antibodies to the hemagglutinin protein, fusion protein, phosphoprotein, matrix protein, and nucleoprotein of measles virus were evaluated as detector antibodies in capture enzyme immunoassays (EIAs) for the detection of specific serum immunoglobulin G (IgG), IgA, and IgM antibodies to measles virus. A pool of monoclonal antibodies to hemagglutinin protein and nucleoprotein proved optimal and was further evaluated. Specific IgM was detected in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and <1% of normal serum specimens. Specffic IgA antibodies were found in 97% of adolescents with clinical measles, 97% of infants 3 weeks postvaccination, and <1% of normal serum specimens. Specific IgA antibodies were found in 97% of clinical measles cases and vaccinees, in 26% of healthy persons, and in 36% of infants 8 months postvaccination; consequently, IgA antibodies were not a useful indicator of recent measles infection. A significant increase in IgG antibodies between paired specimens was detected in 92% of clinical cases and all vaccinees. Only 59% of infant specimens had persistent IgG antibodies as detected by capture EIA at 8 months postvaccination, whereas all specimens had antibodies as detected by hemagglutination inhibition and plaque neutralization. An alternative indirect EIA, in which antigen was directly absorbed to the solid phase, was more sensitive than the capture design, detecting IgG antibodies in all infants postvaccination. When standardized with a microneutralization assay for the detection of persistent antibodies, the indirect IgG EIA gave predictive values for positive and negative tests exceeding 90%. Our capture IgM and indirect IgG EIAs provide a practical combination of serologic tests for the determination of acute measles virus infection and past exposure to measles virus or vaccine, respectively. 1466 on July 6, 2020 by guest

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Enzyme-linked immunosorbent assay for detection of measles antibody

Cited by 45 publications

References 7 publications

Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

Lack of association between transporter associated with antigen processing (TAP) and HLA‐DM gene polymorphisms and antibody levels following measles vaccination

Evaluation of monoclonal antibody-based capture enzyme immunoassays for detection of specific antibodies to measles virus

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