Enzyme-linked immunosorbent assay for cholesteryl ester transfer protein in human serum

Sato, Toshitaka; Fukasawa, Masayoshi; Kinoshita, Makoto; Arai, Hiroyuki; Saeki, Takao; Naraki, Toru; Iwasaki, Yoshihiro; Teramoto, Tamio; Takahashi, Kazuo; Saitō, Yasushi; Inoue, Kazuhide

doi:10.1016/0009-8981(95)06119-5

Cited by 8 publications

(1 citation statement)

References 23 publications

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“…The serum concentrations of apoA-I, apoB, and apoE were determined by a single radial immunodiffusion (43). CETP activity and protein mass of the patients were measured by the methods of Kato et al (44) and Sato et al (45).…”

Section: Methodsmentioning

confidence: 99%

HDL from CETP-deficient subjects shows enhanced ability to promote cholesterol efflux from macrophages in an apoE- and ABCG1-dependent pathway

Matsuura

Wang²,

Chen³

et al. 2006

Journal of Clinical Investigation

263

195

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Genetic deficiency or inhibition of cholesteryl ester transfer protein (CETP) leads to a marked increase in plasma levels of large HDL-2 particles. However, there is concern that such particles may be dysfunctional in terms of their ability to promote cholesterol efflux from macrophages. Recently, the ATP-binding cassette transporter ABCG1, a macrophage liver X receptor (LXR) target, has been shown to stimulate cholesterol efflux to HDL. We have assessed the ability of HDL from subjects with homozygous deficiency of CETP (CETP-D) to promote cholesterol efflux from macrophages and have evaluated the role of ABCG1 and other factors in this process. CETP-D HDL-2 caused a 2-to 3-fold stimulation of net cholesterol efflux compared with control HDL-2 in LXR-activated macrophages, due primarily to an increase in lecithin:cholesterol acyltransferase-mediated (LCAT-mediated) cholesteryl ester formation in media. Genetic knockdown or overexpression of ABCG1 showed that increased cholesterol efflux to CETP-D HDL was ABCG1 dependent. LCAT and apoE contents of CETP-D HDL-2 were markedly increased compared with control HDL-2, and increased cholesterol esterification activity resided within the apoE-HDL fraction. Thus, CETP-D HDL has enhanced ability to promote cholesterol efflux from foam cells in an ABCG1-dependent pathway due to an increased content of LCAT and apoE.

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Section: Methodsmentioning

confidence: 99%