Enzyme‐Linked Immunosorbent Assay (ELISA) for Detecting Antisperm Antibodies in Mice With Testicular Autoimmunity

Itoh, Masahiro; Hiramine, Chiharu; Koseto, Masahiro; Hojo, Kenji

doi:10.1111/j.1600-0897.1989.tb00991.x

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…They suggested that there might be more limited number of these antigens in murine testes an that they are present in a highly insoluble form when compared to guinea pig antigens. Moreover, a murine testicular antigen obtained in a similar way to ASPM, only induced aspermatogenesis in about 15% of actively immunized mice (Itoh et al, 1989). Nevertheless, the consistent cellular and humoral immune response detected in rats immunized with ASPM and the high titres of specific antibodies found in rabbits injected with this immunogen (data not shown) indicate its antigenicity.…”

Section: Discussionmentioning

confidence: 89%

Testicular lesions and lymphocyte subpopulations in rats immunized with a soluble fraction of testicular homogenate

1991

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A soluble fraction obtained from a testicular homogenate by precipitation with ammonium sulphate (ASPM) was emulsified with Freund's complete adjuvant (CFA) and injected into Wistar rats. At 50 days after the first immunization (total of three injections) the animals had developed moderate and multifocal testicular damage, characterized mainly by sloughing of the seminiferous epithelium. A delayed-type hypersensitivity response and circulating antibodies to ASPM were detected at different times with maximum levels at 50 days. The addition of Bordetella pertussis to the immunization did not increase the severity of the lesion but augmented the cellular and humoral immune response to ASPM. The phenotypic characterization of cells present in the lymph nodes draining from the site of immunization in animals injected with CFA alone (control group) revealed an increase in CD8+ T-cells and a low CD4/CD8 ratio. Conversely, rats immunized with CFA plus ASPM (experimental group) exhibited testicular damage and showed a significant decrease in CD8+ cells with a normal CD4/CD8 ratio. In conclusion, rats immunized with a testicular antigen developed focal aspermatogenic lesions and a concomitant specific immune response as well as lymph-node cell variations focused apparently on the CD8+ T-cell subpopulation.

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Section: Discussionmentioning

confidence: 89%

Testicular lesions and lymphocyte subpopulations in rats immunized with a soluble fraction of testicular homogenate

1991

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“…Assays of immune responses DTH to syngeneic TC or sheep erythrocytes was measured on day 40 or on day 5, respectively, by delayed footpad reaction to 1 X 10' TC or sheep erythrocytes as described [9]. Assay of anti-TC antibodies was done with the ELISA procedure at serum dilution of 1:800, and level of the serum antibodies was expressed as an absorbanee value as described [9,23].…”

Section: In Vivo Suppression Of Eao By Adoptive Transfer Ofthe Ts-a Cmentioning

confidence: 99%

Generation and characterization of a continuous line of CD8+ suppressively regulatory T lymphocytes which down-regulates experimental autoimmune orchitis (EAO) in mice

Mukasa

Hiramine

Hojo

1994

Clinical and Experimental Immunology

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SUMMARYWe have previously shown that two injections with viable syngeneic testicular germ cells (TC) alone developed experimental autoimmune orchitis (EAO) in C3H/He mice, and that the induction of antigen-specific tolerance in this EAO model is associated with the generation of antigen-specific suppressively regulatory T (Ts) cells. For the elucidation of the nature of these Ts cells, a murine Ts cell line (designated Ts-A) was established. This line was generated from the spleen cells of C3H/He mice which had received three i.v. injections of a soluble (deaggregated) form of murine testicular antigen (mTA), followed by the repeated selection of these spleen lymphocytes in vitro by stimulation with mTA. Adoptive transfer ofTs-A cells into naive syngeneic mice immediately before the first TC injection was found to downgrade EAO in actively immunized recipients. The transferred Ts-A cells significantly inhibited the cellular immune response to TC in the recipients in an antigen-specific manner, but these cells had no inhibitory effect on the humoral immune response to TC. This line could also inhibit in vitro syngeneic TC-driven proliferation of orchitogenic lymphocytes. Surface phenotype ofthis line was CD8+, CD4-, Thy-i .2+, CD3+, and TCR a,+. These findings may suggest an in vivo role for suppressively regulatory lymphocytes, capable of inhibiting helper T cells, in the regulation of EAO.

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“…Both syngeneic testicular germ cells (TC) and .soluble murine testicular antigens (mTA) were prepared as described [10,11]. Sheep erythrocytes were purchased from Nippon Bio-Test Lab.…”

Section: Aniigensmentioning

confidence: 99%

Suppression of efferent limb of testicular autoimmune response by a regulatory CD4+ T cell line in mice

Itoh

Mukasa

Tokunaga

et al. 1992

Clinical and Experimental Immunology

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SUMMARYA murine Tcell line (designated as CTs) as a mediator of suppression of exfjerimental autoimmune orchitis (EAO) was established. The method of establishment oIC.Ts cell line was preparing spleen eeils from C3H/He mice hyperimtnunized with testicular germ eelts (TC) and the repeated selection of the lymphocytes in vitro by stimulation with mouse testicular antigens (mTA). The CTs cells were Thy 1.2 • .surface immunoglobulin .CD3' .CD4' andCD8 . Thecellscould suppress the induction of EAO when transferred into actively EAO-sensitizcd mice only al the pre-clinical stage of the disease (efferent limb of the autoimmune response). The transferred CTs cells significantly inhibited both cellular and humoral immune responses to TC in the recipients in an antigen-specific manner. The disease suppression by CTs cells was found to depend upon iheir ceil number, and their suppressive activity was markedly augmented by in vitro stimulation with mTA.

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Enzyme‐Linked Immunosorbent Assay (ELISA) for Detecting Antisperm Antibodies in Mice With Testicular Autoimmunity

Cited by 12 publications

References 21 publications

Testicular lesions and lymphocyte subpopulations in rats immunized with a soluble fraction of testicular homogenate

Testicular lesions and lymphocyte subpopulations in rats immunized with a soluble fraction of testicular homogenate

Generation and characterization of a continuous line of CD8+ suppressively regulatory T lymphocytes which down-regulates experimental autoimmune orchitis (EAO) in mice

Suppression of efferent limb of testicular autoimmune response by a regulatory CD4+ T cell line in mice

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