Enzyme-linked immunosorbent assay (ELISA) and a passive haemagglutination test (PHT) for the detection of pseudomonas antibodies in burned patients

Roe, Elizabeth; Jones, Rhys

doi:10.1016/0305-4179(85)90119-6

Burns

1985

DOI: 10.1016/0305-4179(85)90119-6

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Enzyme-linked immunosorbent assay (ELISA) and a passive haemagglutination test (PHT) for the detection of pseudomonas antibodies in burned patients

Elizabeth Roe¹,

Rhys Jones²

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1989

2005

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Cited by 2 publications

References 17 publications

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Immunoprotective extracellular polysaccharides ofKlebsiella aerogenescapsular type K1, expressed inEscherichia coli

Roe

Jones

Saunders

1989

FEMS Microbiology Letters

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A gene library of genomic DNA Klebsiella aerogenes of capsular serotype K1 was constructed in E. coli LE392 using the cosmid pMMB33. Culture filtrates of E. coli recombinants were screened by ELISA for extracellular polysaccharides specific for K. aerogenes K1. Extracellular polysaccharide extracts from K. aerogenes K1 and 3% of the E. coli recombinants contained immunoprotective extracellular polysaccharides (IEP) with similar chemical and immunological properties as shown by gel filtration through Sephacryl 1000, double immunodiffusion and mouse protection tests. IEPs contained no detectable protein, had molecular weights of several hundred million and protected mice against lethal autologous K. aerogenes K1 challenge at a dosage of 10 nanograms per mouse.

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Immunoprotective extracellular polysaccharides ofKlebsiella aerogenescapsular type K1, expressed inEscherichia coli

Roe

Jones

Saunders

1989

FEMS Microbiology Letters

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Pseudomonas aeruginosa: the potential to immunise against infection

Sedlak-Weinstein

Cripps

Kyd

et al. 2005

Expert Opinion on Biological Therapy

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Pseudomonas aeruginosa remains a serious pathogen for specific cohorts of patients where chronic infection is a poor prognostic indicator, such as those with cystic fibrosis, burn wounds or those who are immunocompromised. Significant disease burden is associated with a diverse spectrum of both nosocomial and community-acquired infections. To date, vaccines against P. aeruginosa have shown limited and often conflicting efficacy data, especially against heterologous strains, which are increasingly identified as co-colonisers of biofilms. While few studies have gone beyond Phase II clinical trials, a particular concern is the ability of P. aeruginosa to evade the immune system while provoking an immune response that contributes to the destructive nature of infection. Therefore, vaccine development needs to focus on preventing attachment and colonisation, as well as preventing conversion to a mucoid phenotype that is characteristic of the chronic condition that promotes pathology.

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Enzyme-linked immunosorbent assay (ELISA) and a passive haemagglutination test (PHT) for the detection of pseudomonas antibodies in burned patients

Cited by 2 publications

References 17 publications

Immunoprotective extracellular polysaccharides ofKlebsiella aerogenescapsular type K1, expressed inEscherichia coli

Immunoprotective extracellular polysaccharides ofKlebsiella aerogenescapsular type K1, expressed inEscherichia coli

Pseudomonas aeruginosa: the potential to immunise against infection

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