Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Lyme Disease

Russell, Harold; Sampson, Jacquelyn S.; Schmid, George P.; Wilkinson, Hazel W.; Plikaytis, Brian D.

doi:10.1093/infdis/149.3.465

Cited by 269 publications

(109 citation statements)

References 8 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…In England, positive serology figures ranging from 1% (London) to 7 % (Southampton) were reported [25]. In the United States, the proportion of controls with positive Lyme serology in several studies ranged from none to 10% [10,14,17,26,27]. In an East Coast community, 4 (3.1%) of 129 who were followed during the summer season showed at least fourfold rises in IgG antibody titer, including 2 without clinical LB [14].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Prevalence and Incidence of Clinical and Asymptomatic Lyme Borreliosis in a Population at Risk

Fahrer¹,

Linden²,

Sauvain³

et al. 1991

Journal of Infectious Diseases

129

View full text Add to dashboard Cite

A past history of clinical Lyme borreliosis and the 6-month incidence of clinical and asymptomatic Lyme borreliosis was studied prospectively in a high-risk population. In the spring, blood samples were drawn from 950 Swiss orienteers, who also answered a questionnaire. IgG antiBorrelia burgdorferi antibodies were detected by ELISA. Positive IgG antibodies were seen in 248 (26.1%), in contrast to 3.9%-6.0% in two groups of controls (n = 101). Of the orienteers, 1.9%-3.1% had a past history of definite or probable clinical Lyme borreliosis. Six months later a second blood sample was obtained from 755 participants, 558 (73.9%) of whom were seronegative initially; 45 (8.1%) had seroconverted from negative to positive. Only 1 (2.2%) developed clinical Lyme borreliosis, Among all participants, the 6-month incidence of clinical Lyme borreliosis was 0.8% (6/755) but was much higher (8.1%) for asymptomatic seroconversion (45/558). In conclusion, positive Lyme serology was common in Swiss orienteers, but clinical disease occurred infrequently.Lyme disease, now often called Lyme borreliosis (LB), may affect the skin, heart, joints, and nervous system [1][2][3][4][5][6]. Neurologic, cardiologic, and rheumatologic manifestations such as meningoencephalitis, neuropathy, atrioventricular nodal block, myocarditis, and arthritis may follow in a few weeks to months after erythema migrans (EM). This skin lesion, although characteristic, is by no means an obligatory sign of LB; it was found in only 41%-61% of patients with the disease [7,8]. Arthritis typically is oligoarticular and recurrent but may become chronic and erosive; it predominantly involves the knee joints [3,9].LB is caused by the bite of ticks infected with the spirochete Borrelia burgdorferi [10,11]. The ticks that can transmit disease are part of the Ixodes ricinus complex. The discovery of the spirochete led to serology as an aid in establishing the diagnosis of LB, especially in the absence of EM [12]. In Europe the disorder seems to have a wide and constant distribution [13]. In contrast, in the United States LB may have been confined to three enzootic areas (northeast coast, midwest, California), but cases appear to be increasing and infection to be spreading to new areas. Recognition may also account for some of the apparent increase. LB has now been reported from 35 states. In Long Island, New York, the incidence ofLB was found to be considerably higher than previously recognized [14].Some patients showed inapparent seroconversion after infection. The estimated ratio of apparent to inapparent infection was 1:1 in a study in Great Island, Massachusetts [8].To assess the usefulness of Lyme serology as a diagnostic tool, it would be important to know not only the sensitivity and specificity of the test but also its predictive value. This would imply knowledge about the prevalence and incidence of clinical LB and the ratio of apparent to inapparent infections in populations where the test is applied.We studied past history of clinical LB and frequency of positiv...

show abstract

Section: Discussionmentioning

confidence: 99%

“…An ELISA was done to detect IgG antibody to B. burgdorferi. Briefly, the ELISA antigen was prepared as described by Russell et al [17]. Included in each run was one pooled negative control serum and a strongly positive test serum, which was diluted six times from 1:300 to 1:9600.…”

Section: Anti-borrelia Antibodies (Elisa)mentioning

confidence: 99%

The Prevalence and Incidence of Clinical and Asymptomatic Lyme Borreliosis in a Population at Risk

Fahrer¹,

Linden²,

Sauvain³

et al. 1991

Journal of Infectious Diseases

129

View full text Add to dashboard Cite

show abstract

“…Antibody to B. hermsii was measured in serum specimens obtained from symptomatic visitors by ELISA by use of a whole-cell sonicate preparation of B. hermsii and methods previously described for Borrelia burgdorferi 9 (Quan T, unpublished data). When possible, paired acute-and convalescent-phase serum specimens from symptomatic people were tested, but any single specimen demonstrating an optical density ratio (ODR) of Ն 4.0 compared with a pooled negative control was considered positive.…”

Section: Background and Methodsmentioning

confidence: 99%

Outbreak of tick-borne relapsing fever at the north rim of the Grand Canyon: evidence for effectiveness of preventive measures.

Paul¹,

Maupin²,

Scott-Wright³

et al. 2002

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. An outbreak of tick-borne relapsing fever (TBRF) originating at the North Rim of Grand Canyon National Park was investigated in 1990. To determine risk factors for the disease, almost 7,000 parties of visitors were surveyed; over half responded, representing Ͼ 10,000 people. Fifteen cases of confirmed or probable TBRF were identified in visitors and 2 in employees. All patients except one experienced symptoms after overnight stays in a group of cabins that had not been rodent-proofed after a TBRF outbreak in 1973 (relative risk for visitors [RR] 8.2, 95% confidence interval [CI] 1.1-62). Seven cases of TBRF were associated with a single cabin (RR 98, 95% CI 30-219). Structural flaws and rodent nests were common in the implicated cabins and rare in unaffected cabins. This investigation suggests that measures to rodent-proof cabins at sites where TBRF is endemic prevent reinfestation of cabins by infected rodents and tick vectors, thereby preventing the spread of disease in humans.

show abstract

“…Embora Russell et al (1984) tenham demonstrado igual sensibilidade e especificidade entre imunofluorescência e ELISA, a maioria dos estudos revelam a superioridade do ELISA indireto, em termos de sensibilidade, especificidade e operacionalidade (Stiernstedt et al 1985, Golightly 1993. Magnarelli et al (1984) No método ELISA indireto, borrélias íntegras, antígeno sonicado ou frações protéicas podem ser usado como antígeno, contudo os dois últimos preparados têm mostrado melhores resultados (Bennett 1995, Oksi et al 1995, sendo o antígeno sonicado o mais empregado (Craft et al 1984, Magnarelli et al 1994a.…”

Section: V V V V Borreliose Em Animais Sil Borreliose Em Animaisunclassified

Borrelioses, agentes e vetores

et al. 2000

View full text Add to dashboard Cite

1 RESUMO RESUMO RESUMO RESUMO RESUMO.-As borrelioses são enfermidades infecciosas determinadas por espiroquetas do gênero Borrelia, agentes transmissíveis, principalmente, por carrapatos aos animais e/ou ao homem. Nesta revisão são apresentadas e discutidas as enfermidades determinadas por borrélias, bem como as características gerais das espiroquetas, os aspectos relacionados a transmissão por artrópodes, as enfermidades nos animais domésticos e silvestres, quanto aos aspectos bioló-gicos e patológicos, a doença de Lyme como principal zoonose do grupo, a associação de borrélia com outros agentes hematozoários e os métodos diagnósticos e a epidemiologia comparativa entre dados obtidos no Brasil com os de outros países. Estas borrelioses possuem características patológicas, clínicas e epidemiológicas variadas de acordo à região fisiográ-fica, devido à existência de distintas espécies, genoespécies e cepas; estes aspectos variam ainda em função dos artrópodes vetores, da interação vetor-patógeno e dos ecossistemas distintos.TERMOS DE INDEXAÇÃO: Borreliose, carrapatos, Borrelia spp., febre recurrente, borreliose aviária, borreliose bovina, doença de Lyme, aborto epizoótico bovino.

show abstract

Enzyme-Linked Immunosorbent Assay and Indirect Immunofluorescence Assay for Lyme Disease

Cited by 269 publications

References 8 publications

The Prevalence and Incidence of Clinical and Asymptomatic Lyme Borreliosis in a Population at Risk

The Prevalence and Incidence of Clinical and Asymptomatic Lyme Borreliosis in a Population at Risk

Outbreak of tick-borne relapsing fever at the north rim of the Grand Canyon: evidence for effectiveness of preventive measures.

Borrelioses, agentes e vetores

Contact Info

Product

Resources

About