Enzyme linked immunosorbantassay- lab diagnosis: A review

N, Midhun; K, Sonia Bai J; K, Chakrapani; G, Haripriya; Pathuri, Chandra Kiran Venkata Sai; Ramalakshmi, N Surya

doi:10.18231/j.ijmr.2021.003

Cited by 4 publications

(2 citation statements)

References 3 publications

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“…Several cases from Indonesia, Singapore, and Thailand reported cross-reactivity between SARS-CoV-2 and dengue antibodies, resulting in false-positive detection in dengue cases [14]. For immunosorbent assays such as ELISA, main disadvantages are that it is a labour-intensive procedure; has limited coverage of microbial detection; offers poor reproducibility, generates high rate of false positives, and is expensive [15]. The respiratory panel method is another clinical method in recent use that generates rapid results.…”

Section: Introductionmentioning

confidence: 99%

Metaproteomic analysis of nasopharyngeal swab samples to identify microbial peptides and potential co-infection status in COVID-19 patients

Bihani

Gupta

Mehta

et al. 2023

Preprint

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Respiratory infections disrupt the microbiota in the upper respiratory tract (URT), putting patients at a risk for subsequent infections. During the pandemic, cases of COVID-19 were aggravated by secondary infections because of impaired immunity and medical interventions, which was clearly evident in the second wave of COVID-19 in India. The potential dangers and clinical difficulties of bacterial and fungal secondary infections in COVID-19 patients necessitate microbial exploration of the URT. In this regard, mass spectrometry (MS)-based proteome data of nasopharyngeal swab samples from COVID-19 patients was used to investigate the metaproteome. The MS datasets were searched against a comprehensive protein sequence database of common URT pathogens using multiple search platforms (MaxQuant, MSFragger, and Search GUI/PeptideShaker). The detected microbial peptides were verified using PepQuery, which analyses peptide-spectrum pairs to give statistical output for determining confident microbial peptides. Finally, a protein sequence database was generated using the list of verified microbial peptides for identification and quantitation of microbial peptides and proteins, respectively. The taxonomic analysis of the detected peptides revealed several opportunistic pathogens like Streptococcus pneumoniae, Rhizopus microsporus, Clavispora lusitaniae, and Syncephalastrum racemosum among others. Using parallel reaction monitoring (PRM), we validated a few identified microbial peptides in clinical samples. The analysis also revealed proteins belonging to species like Pseudomonas fluorescens, Enterobacter, and Clostridium to be up-regulated in severe COVID-19 samples. Thus, MS can serve as a powerful tool for untargeted detection of a wide range of microorganisms. Metaproteomic analysis in COVID-19 patients for early identification and characterisation of co-infecting microorganisms can significantly impact the diagnosis and treatment of patients.

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Section: Introductionmentioning

confidence: 99%

Metaproteomic analysis of nasopharyngeal swab samples to identify microbial peptides and potential co-infection status in COVID-19 patients

Bihani

Gupta

Mehta

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Several cases from Indonesia, Singapore, and Thailand reported cross-reactivity between SARS-CoV-2 and dengue antibodies, resulting in false-positive detection in dengue cases . For immunosorbent assays such as ELISA, the main disadvantages are that it is a labor-intensive procedure, has limited coverage of microbial detection, offers poor reproducibility, generates a high rate of false positives, and is expensive . The respiratory panel method is another clinical method in recent use that generates rapid results.…”

Section: Introductionmentioning

confidence: 99%

Metaproteomic Analysis of Nasopharyngeal Swab Samples to Identify Microbial Peptides in COVID-19 Patients

et al. 2023

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During the COVID-19 pandemic, impaired immunity and medical interventions resulted in cases of secondary infections. The clinical difficulties and dangers associated with secondary infections in patients necessitate the exploration of their microbiome. Metaproteomics is a powerful approach to study the taxonomic composition and functional status of the microbiome under study. In this study, the mass spectrometry (MS)-based data of nasopharyngeal swab samples from COVID-19 patients was used to investigate the metaproteome. We have established a robust bioinformatics workflow within the Galaxy platform, which includes (a) generation of a tailored database of the common respiratory tract pathogens, (b) database search using multiple search algorithms, and (c) verification of the detected microbial peptides. The microbial peptides detected in this study, belong to several opportunistic pathogens such as Streptococcus pneumoniae, Klebsiella pneumoniae, Rhizopus microsporus, and Syncephalastrum racemosum. Microbial proteins with a role in stress response, gene expression, and DNA repair were found to be upregulated in severe patients compared to negative patients. Using parallel reaction monitoring (PRM), we confirmed some of the microbial peptides in fresh clinical samples. MS-based clinical metaproteomics can serve as a powerful tool for detection and characterization of potential pathogens, which can significantly impact the diagnosis and treatment of patients.

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An Analytical Protocol for Detecting Antibody Titer Levels in Serum/Saliva by Indirect Enzyme-Linked Immunosorbent Assay (ELISA)

Wang,

Bashiri,

Toth

et al. 2024

Methods in Molecular Biology

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Enzyme linked immunosorbantassay- lab diagnosis: A review

Cited by 4 publications

References 3 publications

Metaproteomic analysis of nasopharyngeal swab samples to identify microbial peptides and potential co-infection status in COVID-19 patients

Metaproteomic analysis of nasopharyngeal swab samples to identify microbial peptides and potential co-infection status in COVID-19 patients

Metaproteomic Analysis of Nasopharyngeal Swab Samples to Identify Microbial Peptides in COVID-19 Patients

An Analytical Protocol for Detecting Antibody Titer Levels in Serum/Saliva by Indirect Enzyme-Linked Immunosorbent Assay (ELISA)

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