Enzyme Kinetics

Bisswanger, Hans

doi:10.1002/9783527622023

Cited by 149 publications

(123 citation statements)

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“…Consequently, the ratio 4K N1 /K N2 can be used to recognize cooperativity: it is greater than 1 for positive cooperativity and less than 1 for negative cooperativity. In terms of generally accepted nomenclature, K N is a "microscopic" binding constant, and K N1 and K N2 are "macroscopic" binding constants (26). The merit of using 4K N1 /K N2 instead of h to characterize cooperativity is that the former parameter is directly related to the free energy change associated with cooperativity, that is the effect of a ligand binding at one site on the binding affinity of the other site (27).…”

Section: Methodsmentioning

confidence: 99%

“…Notably, if Equation 2 instead of Equation 3 is applied to a non-cooperative binding reaction, the fitted values of K N1 and K N2 should differ 4-fold: K N1 ϭ K N /2 and K N2 ϭ 2K N (26). Consequently, the ratio 4K N1 /K N2 can be used to recognize cooperativity: it is greater than 1 for positive cooperativity and less than 1 for negative cooperativity.…”

Section: Methodsmentioning

confidence: 99%

“…As was the case for Equation 2, the ratio 4K m1 /K m2 can be used to recognize catalytic cooperativity: its value is greater than 1 for positive cooperativity and less than 1 for negative cooperativity (26). The applicability of the microscopic/macroscopic constant concept to Michaelis constants results from the formal similarity of the Michaelis-Menten equation to the equilibrium binding equation (28) …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Cystathionine β-Synthase (CBS) Domains Confer Multiple Forms of Mg2+-dependent Cooperativity to Family II Pyrophosphatases

Salminen

Anashkin

Lahti

et al. 2014

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cystathionine β-Synthase (CBS) Domains Confer Multiple Forms of Mg2+-dependent Cooperativity to Family II Pyrophosphatases

Salminen

Anashkin

Lahti

et al. 2014

Journal of Biological Chemistry

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“…Experiments were designed according to the principles described elsewhere (30,31) to facilitate reliable determination of kinetic transport parameters. The data were linearized by plotting reciprocal values for transport rate (V) versus Trp concentration (S) (Fig.…”

Section: Identification Of Deletion Strains With Altered Quininementioning

confidence: 99%

The Antimalarial Drug Quinine Disrupts Tat2p-mediated Tryptophan Transport and Causes Tryptophan Starvation

Khozoie

Pleass

Avery

2009

Journal of Biological Chemistry

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Quinine is a major drug of choice for the treatment of malaria. However, the primary mode of quinine action is unclear, and its efficacy is marred by adverse reactions among patients. To help address these issues, a genome-wide screen for quinine sensitivity was carried out using the yeast deletion strain collection. Quinine-sensitive mutants identified in the screen included several that were defective for tryptophan biosynthesis (trp strains). This sensitivity was confirmed in independent assays and was suppressible with exogenous Trp, suggesting that quinine caused Trp starvation. Accordingly, quinine was found to inhibit [ 3 H]Trp uptake by cells, and the quinine sensitivity of a trp1⌬ mutant could be rescued by overexpression of Trp permeases, encoded by TAT1 and TAT2. The site of quinine action was identified specifically as the high affinity Trp/Tyr permease, Tat2p, with which quinine associated in a Trp-suppressible manner. A resultant action also on Tyr levels was reflected by the Tyr-suppressible quinine hypersensitivity of an aro7⌬ deletion strain, which is auxotrophic for Tyr (and Phe). The present genome-wide dataset provides an important resource for discovering modes of quinine toxicity. That potential was validated with our demonstration that Trp and Tyr uptake via Tat2p is a major target of cellular quinine toxicity. The results also suggest that dietary tryptophan supplements could help to avert the toxic effects of quinine.

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“…A lumped kinetic is used that comprises mass transfer and kinetics. For the enzyme coated packing a reduced ordered bi bi kinetic approach from Bisswanger [32] was employed (2). The kinetic parameters for the coated packing are presented in …”

Section: Model Descriptionmentioning

confidence: 99%

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

Wierschem

Schlimper

Heils

et al. 2017

Chemical Engineering Journal

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Highlights• Butyl butyrate synthesis by Enzymatic Reactive Distillation is possible• Established rate-based model to describe Enzymatic Reactive Distillation • First time validation for an Enzymatic Reactive Distillation process model AbstractFor enzyme-catalyzed reactions, batch processes using stirred tank reactors are the state-ofthe-art production mode. The yield of the process may be limited by reaction equilibrium, product inhibition of the enzyme, low concentrations and possibly low reaction rates, while the recovery of the product may be limited due to thermodynamic constraints such as azeotropes. Using enzymes in an integrated reactive distillation process can overcome these limitations and provides a cost advantage over classic batch reactor processes.The aim of this paper is i) to report the successful pilot-scale experimental validation of an Enzymatic Reactive Distillation (ERD) process for the synthesis of butyl butyrate and ii) to Pilot-Scale Validation of Enzymatic Reactive Distillation for Butyl Butyrate ProductionWierschem et al.2 establish a rate-based model for conceptual process design which can be quickly adapted to other systems.The main novelty is the application of a continuous RD column with enzymes as a heterogeneous catalyst provided in two different types of catalytic packing: loosely filled immobilized enzyme beads in standard packings with catalyst pockets and gauze packings with catalytic coating.Experimental pilot-scale experiments show the feasibility of ERD and allow the comparison of the different packing types based on catalytic performance as well as stability.Furthermore, these experiments are used to validate a predictive rate-based model to describe ERD which can be used to check the sensitivity of process and design parameters as well as to provide a quick adaption to other systems for quick evaluation.

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Enzyme Kinetics

Cited by 149 publications

References 0 publications

Cystathionine β-Synthase (CBS) Domains Confer Multiple Forms of Mg2+-dependent Cooperativity to Family II Pyrophosphatases

Cystathionine β-Synthase (CBS) Domains Confer Multiple Forms of Mg2+-dependent Cooperativity to Family II Pyrophosphatases

The Antimalarial Drug Quinine Disrupts Tat2p-mediated Tryptophan Transport and Causes Tryptophan Starvation

Pilot-scale validation of Enzymatic Reactive Distillation for butyl butyrate production

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