Enzyme immunoassay with enhanced specificity for detection of antibodies to Chlamydia trachomatis

Ossewaarde, Jacobus M.; Vries, Ankje de; Hoek, Janet; Loon, Anton M. van

doi:10.1128/jcm.32.6.1419-1426.1994

Cited by 23 publications

(8 citation statements)

References 45 publications

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“…Immunoglobulin G (IgG) antibodies to cytomegalovirus and IgG and IgA to H. pylori were determined by commercial enzyme immunoassays (Organon Teknika, Boxtel, The Netherlands and Pyloriset EIA-G and EIA-A, Orion Diagnostica, Espoo, Finland, respectively). IgG and IgA antibodies to C. pneumoniae (strain TW-183), C. psittaci (an isolate from a lung biopsy from a psittacosis patient), and C. trachomatis (serovar L2, strain 434-B) were determined by in-house developed enzyme immunoassays [13][14][15]. Briefly, chlamydiae were propagated in six-well microtitre plates.…”

Section: Serological Methodsmentioning

confidence: 99%

Chlamydia pneumoniae is a risk factor for coronary heart disease in symptom-free elderly men, but Helicobacter pylori and cytomegalovirus are not

et al. 1998

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To test the hypothesis that chronic infection with Chlamydia pneumoniae, Helicobacter pylori or cytomegalovirus is associated with coronary heart disease risk in elderly men, a nested case-control study in a cohort investigated in 1985 and 1990 in the town of Zutphen, The Netherlands, was designed. Fifty-four cases with a first diagnosed coronary event between 1985 and 1990, and 108 age-matched control subjects free of coronary heart disease during follow up were included in the study. The overall prevalence of antibodies to cytomegalovirus was 74.7%, to H. pylori 75.9% and to C. pneumoniae 84.0%. A high level of antibodies to C. pneumoniae was associated with an increased coronary heart disease risk (OR = 2.76; 95% CI = 1.31-5.81). This association was stronger in cases developing both myocardial infarction and angina pectoris, than in cases developing only one of these. This association was independent of potential confounders. Antibodies to cytomegalovirus or H. pylori were not associated with coronary heart disease risk. These results support the hypothesis of a role of chronic C. pneumoniae infections in the immunopathogenesis of atherosclerosis.

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Section: Serological Methodsmentioning

confidence: 99%

Chlamydia pneumoniae is a risk factor for coronary heart disease in symptom-free elderly men, but Helicobacter pylori and cytomegalovirus are not

et al. 1998

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“…As an antigen, Chlamydia inclusions of the same strain as that used for the immunization in HeLa 229 cells on microscope slides (30) were used in the indirect immunofluorescence assay. Supernatant was applied undiluted, and antibodies were detected by fluorescein isothiocyanate-labeled sheep antimouse Ig (Cappel, Organon Teknika, Boxtel, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Comparison of two panels of monoclonal antibodies for determination of Chlamydia trachomatis serovars

Ossewaarde¹,

Rieffe²,

Vries³

et al. 1994

J Clin Microbiol

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A panel of monoclonal antibodies was developed for serovar typing of clinical isolates of Chlamydia trachomatis. The panel could distinguish all 15 established serovars from one another, although the hybridomas of the panel were developed by fusions of myeloma cells and spleen cells from mice immunized with antigen derived from the urogenital serovars D to L3. The typing assay was based on a dot enzyme immunoassay, and the monoclonal antibodies that were included in the panel reacted strongly in this assay. A collection of 289 clinical isolates from The Netherlands was typed. The observed serovar frequency distribution was 51 isolates of serovar D (17.6%), 103 isolates of serovar E (35.6%), 62 isolates of serovar F (21.5%), 28 isolates of serovar G (9.9%), 14 isolates of serovar H (4.8%), 2 isolates of serovar I' (0.7%), 20 isolates of serovar J (6.9%), and 9 isolates of serovar K (3.1%). These results were confirmed by typing these isolates with a panel of monoclonal antibodies purchased from the Washington Research Foundation, Seattle. No strain variation was observed within serovar D with both panels. However, restriction fragment length polymorphism analysis of the gene encoding the major outer membrane protein showed that 32 isolates were similar to the prototype D and 17 were similar to the variant D-. The two others showed a new restriction pattern. Our panel of monoclonal antibodies contained one monoclonal antibody that divided the serovar G isolates into two groups. This differentiation was confirmed by restriction fragment length polymorphism analysis, confining this difference to a known sequence variation in variable domain IV. These data support the subdivision of serovar G into serovars G (prototype strain UW-57) and Ga (prototype strain IOL-238).

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“…Отже, комплексне обстеження пацієнтів на наявність гуморальної імунної відповіді на Ch. trachomatis передбачає тестування сироватки (плазми) крові на вміст специфічних антитіл усіх трьох класів (Ossewaarde, 1994;Isakov et al, 2012;Kamel, 2013).…”

Section: вступunclassified

“…За літературними даними (Ossewaarde, 1994;Kamel, 2013), імуноферментні набори для виявлення специфічних антитіл класу IgM та IgA до Ch. trachomatis у сироватці (плазмі) крові людини побудовані за принципом непрямого ІФА.…”

Section: вступunclassified

Synthetic positive controls for ELISA test kits for detection of IgA and IgM antibodies to Chlamydia trachomatis

Galkin

Gorshunov

Besarab

2015

VDNUBM

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The enzyme-linked immunosorbent assay (ELISA) is the most informative and versatile method of serological diagnostics. The possibility of detecting by ELISA specific antibodies of different classes allow to differentiate primary infectious process and its remission, exacerbation and chronic disease (holding of differential diagnosis). This approach is implemented in the methodology for evaluation of patients for presence of humoral immune response against the causative agent of urogenital chlamydiosis. As with other infections immediately after Chlamydia trachomatis infection the specific IgM antibodies are formed, and subsequently basic projective antibodies of IgG class are synthesized. However, at exacerbation of chronic urogenital chlamydiosis specific IgA antibodies can be synthesized. That is why comprehensive evaluation of patients for presence of humoral immune response to Ch. trachomatis involves plasma testing of specific antibodies of all three classes. The essential problem in the production of ELISA diagnostic kits is obtaining of positive control. The classic version of positive control is human blood plasma containing specific antibodies. But specific IgM- and IgA-positive sera are deficit raw materials. This fact can significantly limit the production of diagnostic kits, especially in case of large-scale manufacture. We have suggested methodological approach to use of synthetic positive controls in indirect ELISA kits based on conjugate of normal human IgM (IgA) and monoclonal antibodies against major outer membrane protein of Ch. trachomatis. It was found that it’s possible to realize such task by means of NHS ester-maleimide-mediated conjugation (by sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) and reductive amination-mediated conjugation (by sodium periodate). It was found that synthetic positive controls obtained by different methods are characterized by higher titer compared to IgM- and IgA-positive high-titer serum. However, positive control obtained by NHS ester-mediated maleimide conjugation has the best titration profile characteristics, at the release time and after one-week storage at37 °C.

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Enzyme immunoassay with enhanced specificity for detection of antibodies to Chlamydia trachomatis

Cited by 23 publications

References 45 publications

Chlamydia pneumoniae is a risk factor for coronary heart disease in symptom-free elderly men, but Helicobacter pylori and cytomegalovirus are not

Chlamydia pneumoniae is a risk factor for coronary heart disease in symptom-free elderly men, but Helicobacter pylori and cytomegalovirus are not

Comparison of two panels of monoclonal antibodies for determination of Chlamydia trachomatis serovars

Synthetic positive controls for ELISA test kits for detection of IgA and IgM antibodies to Chlamydia trachomatis

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