Enzyme Immunoassay of Angiotensin II in Human Plasma.

Yasunaga, Fumitaka; Kawano, Ayumi; Morikawa, Norifumi; Takeyama, Masaharu

doi:10.1248/cpb.40.792

Cited by 3 publications

(3 citation statements)

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“…Ang II in biological samples has been commonly measured by immunoassays, which are notoriously susceptible to interference (Lawrence et al, 1990; Nussberger et al, 1985; Templ & Templ, 1991; Voelker et al, 1994; Volland et al, 1999; Yasunaga et al, 1992). Direct measurement of Ang II by LCMS methods can overcome the specificity problem and was originally reported in 2007 (Ali et al, 2014; Bernstone et al, 2021; Boccardo et al, 2015; Cui et al, 2007; Hildebrand et al, 2013; Lee et al, 2013; Nicola et al, 1995; Schulz et al, 2014; Shen et al, 2019; van Rooyen et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Section: Reference Intervalsmentioning

confidence: 99%

“…Upon identifying the Ang II amino acids sequence in 1956 (Hall, 2003), Ang II antibodies became possible. Immunoassays to quantitate Ang II in different biological matrices have been introduced since 1967 (Catt & Cain, 1967; Towbin et al, 1995; Volland et al, 1999; Yasunaga et al, 1992). However, the anti‐Ang II antibodies used in these assays were susceptible to cross‐reactivity with homologies from the RAS metabolic pathway (Lawrence et al, 1990; Nussberger et al, 1985; Templ & Templ, 1991; Voelker et al, 1994; Volland et al, 1999; Yasunaga et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Quantitation of plasma angiotensin II in healthy Chinese subjects by a validated liquid chromatography tandem mass spectrometry method

Tsui

Ching‐Wan

et al. 2022

Biomedical Chromatography

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Quantitation of plasma angiotensin (Ang) II, the active mediator of the reninangiotensin system, is challenging owing to its low physiological concentration. We report a validated liquid chromatography-mass spectrometry (LCMS) method to overcome this challenge. Ang II was extracted from EDTA plasma by an offline solidphase extraction procedure with a Waters MAX μElution plate. LCMS quantitation was performed on the Waters TQS system, monitoring the 3+ ions of the peptide.The analytical performance of the LCMS method was validated. The stability of Ang II was studied with or without the presence of a protease inhibitor. Local reference intervals were established from 143 healthy normotensive subjects (57% female, 21-60 years old). The Ang II LCMS method had a measurable range of 3.3-700 pmol/L.The between-batch precision coefficient of variation was <7% over Ang II concentrations of 8.6-110 pmol/L. No significant matrix interference and carryover were observed. There was no significant difference in Ang II concentration in EDTA blood and plasma for at least 2h and 1 h at room temperature, respectively. Ang II was stable for at least 1 year when stored at À80 C, with or without the protease inhibitor.

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