Enzyme immunoassay for the measurement of histamine

Chevrier, D.; Guesdon, Jean‐Luc; Mazié, J. C.; Avraméas, S

doi:10.1016/0022-1759(86)90223-1

Cited by 33 publications

(8 citation statements)

References 13 publications

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“…Many methods have been used for the determination of histamine: fluorometry (3), HPLC-fluorometry (4), HPLC-luminometry (5), radioenzymatic assay (6), gas chromatography-mass spectrometry (7), radioimmunoassay (8), and enzyme immunoassay (9). Because some methods are designed for relatively large sample volumes, they are unsuitable for the detection of very small amounts of histamine in small sample volumes (i.e., less than 10 l).…”

mentioning

confidence: 99%

Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase

Iwaki

Ogasawara

Kurita

et al. 2002

Analytical Biochemistry

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confidence: 99%

Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase

Iwaki

Ogasawara

Kurita

et al. 2002

Analytical Biochemistry

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“…This may thus suggest the possible development of an ELJSA for sensitive quantification of HA in biological fluids such as urine and blood, the latter being reacted with GA and NaBH, prior to the ELISA assay. Actually, it has been reported that HA was measured by means of radioimmunoassays and enzyme immunoassays, after being led to the chemical derivatives with succinyl glycinamide iV-hydroxysuccinimide ester (33), and 1,4-benzoquinone (34,35) respectively, which were the cross-linkers used for HA coupling with a carrier protein in the preparation of each HA antigen.…”

Section: Resultsmentioning

confidence: 99%

Glutaraldehyde (GA)-Hapten Adducts, but without a Carrier Protein, for Use in a Specificity Study on an Antibody against a GA-Conjugated Hapten Compound: Histamine Monoclonal Antibody (AHA-2) as a Model

Fujiwara

Murata

Yagisawa

et al. 1999

Journal of Biochemistry

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In our recent study on monoclonal antibodies (mAbs AHA-1-5) against glutaraldehyde (GA)-conjugated histamine (HA), we identified one mAb (AHA-2) which can detect neuronal HA in the rat brain with an immunocytochemistry method (ICC) [Fujiwara et al. (1999) J. Biochem. 126, 503-509]. In the present study the specificity of AHA-2 mAb for use for ICC has been examined by means of competitive experiments involving HA and analogs, all of which had been allowed to react with GA followed by sodium borohydride, but not allowed to couple with the carrier protein. It was demonstrated that the antibody distinguished alterations in the chemical structure of the molecule, showing decreased immunoreactivity with all the GA-adducts of (R)-(-)-alpha-methylhistamine, 1- and 3-methylhistamine, L-histidine, and 1- and 3-methyl-L-histidine. On the other hand, AHA-1 mAb only reacted with GA-adducts of 3-MeHA (3-MeHA-GA) and HA (HA-GA), to almost the same degree, in relatively high concentration ranges. AHA-3, 4, and 5 mAbs reacted about 10-times more strongly with 1-MeHA-GA than with HA-GA, but reacted very little or not at all with the other analogs. These results may suggest that AHA-2 mAb recognized both the non-substituted imidazole and alpha-methine groups of a HA molecule in addition to the conjugation site of GA including the part(s) reduced with NaBH(4), and especially the imidazole group more strictly than the other mAbs. This may partly explain why AHA-2, among the five AHA mAbs, can detect neuronal HA with an ICC method. The present ELISA method for GA-hapten adducts should be applicable to other antibodies against GA-conjugated biologically active amines or amino acids, thus allowing the study of antibody specificity for ICC more easily and accurately than was previously possible with hapten-protein conjugates as antigens.

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“…The other techniques used to quantify histamine in other fluids take too long [29,31], give cross-reactivity [26,27,30,35,36], need sophisticated equipment and elaborate procedures [38], or possess a higher limit of sensitivity [41].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Determination of Histamine in Tears during Conjunctivitis by a Novel HPLC Method

Venza¹,

Visalli²,

Ceci³

et al. 2004

Ophthalmic Res

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The histamine content of tears of healthy sex- and age-matched subjects and patients affected by allergic or nonallergic inflammatory ocular diseases was determined through a new competitive reverse-phase high-performance liquid chromatography (HPLC) method. Tear samples from 50 healthy subjects, 30 patients affected by seasonal allergic conjunctivitis, 12 patients with bacterial conjunctivitis associated with Haemophilus influenzae and 8 patients with bacterial conjunctivitis associated with Streptococcus pneumoniae were analyzed for histamine concentration by o-phthaldialdehyde precolumn derivatization-based HPLC. In physiological conditions, the tear histamine content was low (2.26 ng/ml) and did not vary in relation to age and sex. Histamine levels were significantly higher in all the patients studied, to a greater extent in those affected by allergic (23.61 ng/ml) or Haemophilus influenzae-associated (21.53 ng/ml) conjunctivitis.

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Enzyme immunoassay for the measurement of histamine

Cited by 33 publications

References 13 publications

Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase

Real-Time Monitoring of Histamine Released from Rat Basophilic Leukemia (RBL-2H3) Cells with a Histamine Microsensor Using Recombinant Histamine Oxidase

Glutaraldehyde (GA)-Hapten Adducts, but without a Carrier Protein, for Use in a Specificity Study on an Antibody against a GA-Conjugated Hapten Compound: Histamine Monoclonal Antibody (AHA-2) as a Model

Quantitative Determination of Histamine in Tears during Conjunctivitis by a Novel HPLC Method

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