Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon

Effects of Dietary Arachidonic Acid on the Reproductive Physiology of Female Atlantic Cod (Gadus morhua L.).

“…Steroids were extracted from plasma samples as described by Hyllner et al (1994), dissolved in 1 ml of buffer (0.1 M phosphate pH 7.4, 0.4 M NaCl, 1 mM EDTA, 0.1% BSA) by heating (60°C; 10 min) and stored at À20°C until analysis by enzyme-linked immunosorbent assay (ELISA) (Cuisset et al, 1994;Rodriguez et al, 2000) validated for cod (Dahle et al, 2003). Extraction efficiencies were 95% for T and 94% for E2, respectively.…”

Effects of Continuous Light on the Reproductive System of European Sea Bass Gauged by Alterations of Circadian Variations during Their First Reproductive Cycle

“…However, and despite their importance, no 14 data are yet available concerning the daily rhythms of reproductive hormones in fish kept under LL. 15 This is especially relevant considering the great differences that exist between the reproductive 16 effects of long photoperiods, such as that applied in the study by Bayarri et al (2004), and LL, 17 which delays or inhibits puberty and reproductive hormones, respectively. 18 The characterization and temporal appearance of male sea bass gonadal stages, as well as ).…”

“…Therefore, the study of reproductive hormone circadian variations may provide interesting 14 information that could contribute to a better understanding of reproductive function of fish and 15 other species. 16 The European sea bass (Dicentrarchus labrax) is a teleost fish species that is highly valued 17 in Mediterranean aquaculture. A high percentage of the males of this species mature precociously 18 during their first year of life, before reaching commercial size, which in this species occurs at 19 around 18 months of age (Carrillo et al, 1995).…”

“…The sensitivity of the assay was approximately 0.6 ng/ml, and the intra and inter- 15 assay coefficients of variation were approximately 11% and 13%, respectively. 16 Steroid assays 17 A specific enzyme immunoassay (EIA) developed by Rodríguez et al (2000a) for sea bass 18 was used to determine plasma testosterone levels. Plasma 11-ketotestosterone levels were analyzed 19 using an EIA originally developed for Siberian sturgeon (Cuisset et al, 1994), which was modified 20 for sea bass, and using a final dilution of 1:320000 for primary antibodies and 1:10 for the tracer 21 (Cayman Chemicals, MI, USA) (Rodríguez et al, 2001a).…”

CARACTERIZACIÓN BIOLÓGICA DE LAS GONADOTROFINAS DE LUBINA (Dicentrarchus labrax) Y DESARROLLO DE HERRAMIENTAS BIOTECNOLÓGICAS E INMUNOLÓGICAS PARA SU ESTUDIO

“…al, 2005). The protocol was similar to that described by Cuisset et al (1994) except that primary antibodies were used at a final dilution of 1:200,000 and the tracer (Cayman Chemicals, MI, USA) was diluted at 1:10 E.U. /ml.…”