Enzyme Immobilized Nanomaterials: An Electrochemical Bio-Sensing and Biocatalytic Degradation Properties Toward Organic Pollutants

Suresh, R.; Rajendran, Saravanan; Khoo, Kuan Shiong; Soto-Moscoso, Matias

doi:10.1007/s11244-022-01760-w

Cited by 11 publications

(7 citation statements)

References 110 publications

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“…For example, polystyrene sulfonate can be dropped onto the electrode surface to immobilize oxidases [ 26 ], polyethyleneimine was applied to immobilize enzymes on agarose gels [ 27 ], bilirubin oxidase was introduced into a Nafion and crosslinked with glutaraldehyde to form a stable electrochemical interface [ 28 ]. The immobilization of enzymes can be classified as physical and chemical methods [ 29 ]. Adsorption [ 30 ], entrapment, and encapsulation are physical immobilization methods [ 31 ], whereas covalent bonding [ 32 ], crosslinking, and electrostatic attraction are classified as chemical immobilization methods ( Figure 4 ), and the immobilization methods for enzyme-based electrochemical gas sensors were described in Table 1 .…”

Section: The Immobilization Methods Of Enzymementioning

confidence: 99%

Design and Construction of Enzyme-Based Electrochemical Gas Sensors

Zhang,

Chen,

Xing

et al. 2023

Molecules

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The demand for the ubiquitous detection of gases in complex environments is driving the design of highly specific gas sensors for the development of the Internet of Things, such as indoor air quality testing, human exhaled disease detection, monitoring gas emissions, etc. The interaction between analytes and bioreceptors can described as a “lock-and-key”, in which the specific catalysis between enzymes and gas molecules provides a new paradigm for the construction of high-sensitivity and -specificity gas sensors. The electrochemical method has been widely used in gas detection and in the design and construction of enzyme-based electrochemical gas sensors, in which the specificity of an enzyme to a substrate is determined by a specific functional domain or recognition interface, which is the active site of the enzyme that can specifically catalyze the gas reaction, and the electrode–solution interface, where the chemical reaction occurs, respectively. As a result, the engineering design of the enzyme electrode interface is crucial in the process of designing and constructing enzyme-based electrochemical gas sensors. In this review, we summarize the design of enzyme-based electrochemical gas sensors. We particularly focus on the main concepts of enzyme electrodes and the selection and design of materials, as well as the immobilization of enzymes and construction methods. Furthermore, we discuss the fundamental factors that affect electron transfer at the enzyme electrode interface for electrochemical gas sensors and the challenges and opportunities related to the design and construction of these sensors.

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Section: The Immobilization Methods Of Enzymementioning

confidence: 99%

Design and Construction of Enzyme-Based Electrochemical Gas Sensors

Zhang,

Chen,

Xing

et al. 2023

Molecules

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“…The configuration, performances, and applications of enzyme-based biosensors were discussed in several recent reviews [ 6 , 8 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 ]. Both the direct catalytic activity and the inhibition of enzymatic activity were exploited in these applications [ 6 , 8 , 76 , 90 ].…”

Section: Characteristics Of Extremozyme-based Biosensors For Environm...mentioning

confidence: 99%

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Purcarea,

Ruginescu,

Banciu

et al. 2024

Biosensors

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Extremozymes combine high specificity and sensitivity with the ability to withstand extreme operational conditions. This work presents an overview of extremozymes that show potential for environmental monitoring devices and outlines the latest advances in biosensors utilizing these unique molecules. The characteristics of various extremozymes described so far are presented, underlining their stability and operational conditions that make them attractive for biosensing. The biosensor design is discussed based on the detection of photosynthesis-inhibiting herbicides as a case study. Several biosensors for the detection of pesticides, heavy metals, and phenols are presented in more detail to highlight interesting substrate specificity, applications or immobilization methods. Compared to mesophilic enzymes, the integration of extremozymes in biosensors faces additional challenges related to lower availability and high production costs. The use of extremozymes in biosensing does not parallel their success in industrial applications. In recent years, the “collection” of recognition elements was enriched by extremozymes with interesting selectivity and by thermostable chimeras. The perspectives for biosensor development are exciting, considering also the progress in genetic editing for the oriented immobilization of enzymes, efficient folding, and better electron transport. Stability, production costs and immobilization at sensing interfaces must be improved to encourage wider applications of extremozymes in biosensors.

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“…One of the main challenges is achieving an effective enzyme immobilization into a matrix with a large surface area while providing a good mechanical stability. Therefore, different immobilization techniques have been developed consisting on entrapment, adsorption, and encapsulation mechanisms (based on physical interactions), or crosslinking, covalent bonds formation, and electrostatic attraction (based on chemical interactions) [111], as displayed in Figure 6. Regarding the possible supporting materials, nanomaterials are placed on the top owing to their huge specific surface area, thus, aerogels being interesting and promising candidates.…”

Section: Enzymes-based Biosensormentioning

confidence: 99%

Trends on Aerogel-Based Biosensors for Medical Applications: An Overview

Almeida,

Merillas,

Pontinha

2024

IJMS

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Aerogels are unique solid-state materials composed of interconnected 3D solid networks and a large number of air-filled pores. This structure leads to extended structural characteristics as well as physicochemical properties of the nanoscale building blocks to macroscale, and integrated typical features of aerogels, such as high porosity, large surface area, and low density, with specific properties of the various constituents. Due to their combination of excellent properties, aerogels attract much interest in various applications, ranging from medicine to construction. In recent decades, their potential was exploited in many aerogels’ materials, either organic, inorganic or hybrid. Considerable research efforts in recent years have been devoted to the development of aerogel-based biosensors and encouraging accomplishments have been achieved. In this work, recent (2018–2023) and ground-breaking advances in the preparation, classification, and physicochemical properties of aerogels and their sensing applications are presented. Different types of biosensors in which aerogels play a fundamental role are being explored and are collected in this manuscript. Moreover, the current challenges and some perspectives for the development of high-performance aerogel-based biosensors are summarized.

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Enzyme Immobilized Nanomaterials: An Electrochemical Bio-Sensing and Biocatalytic Degradation Properties Toward Organic Pollutants

Cited by 11 publications

References 110 publications

Design and Construction of Enzyme-Based Electrochemical Gas Sensors

Design and Construction of Enzyme-Based Electrochemical Gas Sensors

Extremozyme-Based Biosensors for Environmental Pollution Monitoring: Recent Developments

Trends on Aerogel-Based Biosensors for Medical Applications: An Overview

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