Enzyme immobilization on anodic aluminum oxide/polyethyleneimine or polyaniline composites

Oliveira, Givanildo Bezerra; Filho, J. L. Lima; Chaves, M. E. C.; Azevêdo, W.M. de; Carvalho, Luiz Bezerra de

doi:10.1016/j.reactfunctpolym.2007.10.009

Cited by 48 publications

(32 citation statements)

References 16 publications

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“…There are only few studies that report on immobilization of trypsin by entrapping within [34] or coating [35] on the membrane polymer. Furthermore, the covalent immobilization of trypsin on polymer membranes was reported [36][37][38]. However, the described covalent immobilization technique requires several synthesis steps including critical reagents.…”

Section: Polymers 2015 7mentioning

confidence: 99%

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Biocatalytic Self-Cleaning Polymer Membranes

et al. 2015

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Polymer membrane surfaces have been equipped with the digestive enzyme trypsin. Enzyme immobilization was performed by electron beam irradiation in aqueous media within a one-step method. Using this method, trypsin was covalently and side-unspecific attached to the membrane surface. Thus, the use of preceding polymer functionalization and the use of toxic solvents or reagents can be avoided. The resulting membranes showed significantly improved antifouling properties as demonstrated by repeated filtration of protein solutions. Furthermore, the biocatalytic membrane can be simply "switched on" to actively degrade a fouling layer on the membrane surface and regain the initial permeability. The membrane pore structure (pore size and porosity) was neither damaged by the electron beam treatment nor blocked by the enzyme loading, ensuring a stable membrane performance.

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Section: Polymers 2015 7mentioning

confidence: 99%

“…Testing the activity of immobilized trypsin wa performed according to the method of Oliveira et al [38]. The substrate solution was prepared by mixin 0.3 mM BAPNA, 1 mL DMSO and 9 mL 50 mM TRIS-HCl buffer containing 10 mM CaCl 2 · 2H 2 O, pH 8.…”

Section: Preparation and Characterization Of Biocatalytic Membranesmentioning

confidence: 99%

Biocatalytic Self-Cleaning Polymer Membranes

et al. 2015

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“…There are only few studies reported that used ZnO, copper and aluminum oxide (43)(44)(45). Patterned growth of ZnO nanorods using photoresist was successfully performed on epitaxial single crystalline ZnO using a hydrothermal method and glucose oxidase was immobilized (45).…”

Section: Other Metallic Materialsmentioning

confidence: 99%

“…After surface chemical polymerization, anodic aluminum oxide could be supports for enzyme immobilization (44). Since artificial membranes have high surface area per unit volume and possibility to combine separation with chemical reaction, it has interested biotechnologist in their attempt to find carriers for the immobilization of biocatalysts (46).…”

Section: Other Metallic Materialsmentioning

confidence: 99%

Inorganic nanomaterial-based biocatalysts

Lee

Lee²,

Chang³

et al. 2011

BMB Reports

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Over the years, nanostructures have been developed to enable to support enzyme usability to obtain highly selective and efficient biocatalysts for catalyzing processes under various conditions. This review summarizes recent developments in the nanostructures for enzyme supporters, typically those formed with various inorganic materials. To improve enzyme attachment, the surface of nanomaterials is properly modified to express specific functional groups. Various materials and nanostructures can be applied to improve both enzyme activity and stability. The merits of the incorporation of enzymes in inorganic nanomaterials and unprecedented opportunities for enhanced enzyme properties are discussed.

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“…Autotaxin activity was calculated by using the following extinction coefficient for choline at 490 nm as 1.587/μM/cm [16]. The enzyme activity is expressed as Choline (nmol/mL/min) = (Absorbance of sample − Absorbance of blank) × 1000 × 50 1.587 × 10…”

Section: Calculationsmentioning

confidence: 99%

Effect of histidine on autotaxin activity in experimentally induced liver fibrosis

El-Batch

Ibrahim

Said

2010

J Biochem & Molecular Tox

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The aim of this study was to explain whether serum autotaxin (ATX) activity might be a target for regulation of liver fibrosis and to evaluate the hepatoprotective and antifibrotic effects of histidine in thioacetamide (TAA)-induced liver fibrosis in rats. This study was carried out on 100 Wistar Albino rats, classified into five groups, each containing 20 rats: Group I (control group), Group II: rats were given histidine intraperitoneally, Group III: rats were injected intraperitoneally with TAA, Group IV: rats were injected with L-histidine together with TAA, and Group V: rats were injected with TAA for 1 month then treated with intraperitoneal injection of L-histidine for another month. At the end of experiment, blood and liver were collected for determination of some liver enzymes, plasma total antioxidant capacity (TAC), serum ATX activity, and liver tissue hydroxyproline. Thioacetamide treatment caused significant increases in liver enzymes, ATX activities, and liver hydroxyproline, but a significant decrease in plasma's TAC. Upon treatment with histidine, a significant decrease in liver enzymes, ATX activities, and liver hydroxyproline was observed with a significant increase in plasma TAC in Group IV and a significant decrease in Group V. Histidine as an antioxidant has a protective effect on TAA-induced liver fibrosis; it is beneficial in rats not only by inhibition of collagen synthesis and increasing TAC but also by inhibition of ATX activities thus reducing its capacity to produce lysophosphatidic acid, which has a role in liver fibrosis. C 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:143-150, 2011; View this article online at wileyonlinelibrary.com.

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Enzyme immobilization on anodic aluminum oxide/polyethyleneimine or polyaniline composites

Cited by 48 publications

References 16 publications

Biocatalytic Self-Cleaning Polymer Membranes

Biocatalytic Self-Cleaning Polymer Membranes

Inorganic nanomaterial-based biocatalysts

Effect of histidine on autotaxin activity in experimentally induced liver fibrosis

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