Enzyme immobilization for biodiesel production

Zhang, Baohua; Weng, Yanqing; Xu, Hong; Mao, Zhiping

doi:10.1007/s00253-011-3672-x

Cited by 158 publications

(104 citation statements)

References 62 publications

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“…Repeated-batch fermentation tests were carried out to evaluate the relationship between immobilized yeast and residual sugar in the concentration of saccharification liquid (Zhang et al 2012). The calcium alginate beads of immobilized yeast were washed with PBS at pH 3.…”

Section: Repeated-batch Fermentationmentioning

confidence: 99%

Dynamic Analysis of Bioethanol Production from Corn Stover and Immobilized Yeast

Tian¹,

Chen²

2016

BioResources

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The use of low cost and abundant corn stover in yeast fermentation can reduce product costs. In this study, bioethanol was produced from a hydrolysate of corn stover using immobilized yeast. A kinetic model was established for the total reducing sugar consumption and the production of bioethanol. The parameter estimation for kinetic modeling considered the main process variables during bioethanol production from corn stover. Total reducing sugar concentrations decreased exponentially in the bioethanol fermentation for 6 h; consumption was more than 90%. To use kinetic modelling of yeast growth for bioethanol fermentation, the value of μmax reached 0.2891 h -1 , and the matrix inhibition constant (KIS) and production inhibition constant (KIP) were 8.9154 g/dm 3 and 0.00676 g/dm 3 , respectively. To use kinetic modelling of fermentation time on bioethanol, the maximum ratio of bioethanol production rate (qmax) reached 1.427 g/g•L. However, KIS was 2.813 g/dm 3 , and KIP was 0.0149 g/dm 3 .

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Section: Repeated-batch Fermentationmentioning

confidence: 99%

Dynamic Analysis of Bioethanol Production from Corn Stover and Immobilized Yeast

Tian¹,

Chen²

2016

BioResources

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“…Although biodiesel has been successfully produced chemically, there are still several process issues that require further development, such as glycerol recovery and the removal of inorganic salts. The disadvantages caused by chemical catalysts are largely avoided by using lipases as catalysts (Zhang et al, 2012;Fjerbaek et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

Tiosso¹,

Carvalho

Castro

et al. 2014

Braz. J. Chem. Eng.

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-This work reports the use of commercially available immobilized lipase preparations (Novozym® 435 and Lipozyme TL IM, both from Novozymes, and Lipase PS IM from Amano) as catalysts in the transesterification reaction of different alkyl-chain triglycerides with ethanol. The ethanolysis of native oils from Brazilian Amazon plants andiroba (Carapa guianensis), babassu (Orbignya sp.), jatropa (Jatropha curcas), and palm (Elaeis sp.) was studied in a solvent-free system. In a typical reaction, the immobilized preparations were added to the mixture of vegetable oil-to-ethanol in a molar ratio of 1:9. The reactions were performed at 50 °C for a maximum period of 48 h. Under the conditions used, all the immobilized lipase preparations were able to generate the main esters of fatty acids present in the tested feedstocks, and both the reaction rate and ester yield were dependent on the source of lipase and vegetable oil. The viscosity values for the samples obtained in each reaction displayed a consistent reduction in relation to their original feedstocks, which also confirms the high conversion of triglycerides to ethyl esters (99.8-74.0%). The best performances were obtained with Amano PS IM and Novozym® 435, with the biodiesel samples from the babassu and jatropha oils exhibiting viscosity values in accordance with those predicted by the technical standards of ASTM D6751 (1.9-6.0 mm 2 /s). Lipozyme TL IM displayed an unsatisfactory performance, indicating that the conditions of the transesterification reaction should be improved. This comparative study using different catalysts and several vegetable oil sources with varying fatty acid compositions is particularly important for all tropical countries with a diversity of native vegetable oil sources.

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“…However, the applicability of the chemical processes used to create biodiesel has been restricted due to substantial energy requirements, challenges in retrieving catalyzers, and various environmental barriers [3]. Enzymatic methods, including lipase catalysis, are more advantageous than chemical processes due to their lower energy consumption, easier product retrieval, environmentally friendly means, and adaptability to a broad range of crude substances, particularly those with large free fatty acid content [4]. Despite the multiple advantages of lipases, the high price of enzyme catalysts is a significant barrier in biodiesel manufacturing.…”

Section: Introductionmentioning

confidence: 99%

X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production

Adnan

et al. 2018

Catalysts

103

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Abstract:This study presents a one-step encapsulation method for synthesizing X-shaped zeolitic imidazolate frameworks (ZIF-8) and immobilizing Rhizomucor miehei lipase (RML). We proved that the morphological structure of ZIF-8 had changed after immobilization with enhanced characterization using a field-emission scanning electron microscope, an energy-dispersive spectrometer, a transmission electron microscope, a Fourier transform infrared spectrometer, and powder X-ray diffraction. The surface area and pore size of the carrier were investigated before and after immobilization using Brunauer-Emmett-Teller and Barrett-Joyner-Halenda methods, respectively. RML@ZIF-8 exhibited high recovery activity of up to 2632%, representing a 26-fold increase in its free lipase. Encapsulated RML was used for biodiesel production from soybean oil in an isooctane system with a conversion yield of 95.6% under optimum conditions. The resulting reusability of the immobilized enzyme indicated no substantial decline in the conversion yield, which remained at 84.7% of the initial activity after 10 cycles. The stability and high performance of the immobilized enzyme are attributed to the harmony between RML and ZIF-8 based on the easy synthesis of ZIF-8 and the short time required to immobilize RML.

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Enzyme immobilization for biodiesel production

Cited by 158 publications

References 62 publications

Dynamic Analysis of Bioethanol Production from Corn Stover and Immobilized Yeast

Dynamic Analysis of Bioethanol Production from Corn Stover and Immobilized Yeast

Utilization of immobilized lipases as catalysts in the transesterification of non-edible vegetable oils with ethanol

X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production

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