Enzyme hydrolysis of soybean oil in a slug flow microsystem

Čech, Jiří; Schrott, Walter; Slouka, Zdeněk; Přibyl, Michal; Brož, Michal; Kuncová, Gabriela; Šnita, Dalimil

doi:10.1016/j.bej.2012.06.015

Cited by 29 publications

(22 citation statements)

References 51 publications

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“…of 0.75 mm are quite low, see Figure 6. As we have found in our previous work, 21 an important factor that affects the reaction rate is the surface density a of the water-oil interface. The surface density is often called the surface-to-volume ratio.…”

Section: F Interfacial Areamentioning

confidence: 73%

“…In agreement with the results of our previous study, the enzyme reaction destabilizes slug flow in the 2PO arrangement (Figure 8 variations of surface tension. 21 We observe large slugs of different sizes of one or the other phase at the capillary outlet as the slugs tend to merge by irregular manner. These irregularities work against the purpose of the microchip use, e.g., mass transfer intensity is no longer uniform or the Taylor dispersion is not efficiently suppressed.…”

Section: F Interfacial Areamentioning

confidence: 99%

“…It recently happened in our microreactor with a hydrophobic microcapillary because the surface tension significantly decreased in the course of an enzyme reaction. 21 Slug instabilities can lead to an insufficient reproducibility in the composition of reaction products due to irreproducible mass transfer conditions in particular slugs. The main idea of the suggested 3P system is to separate a fine liquid-liquid dispersion into small discrete volumes by means of nitrogen slugs.…”

Section: B Microfluidic Systemsmentioning

confidence: 99%

“…Details on the HPLC analysis are specified in Ref. 21. Here, we define the soybean oil conversion as the ratio of the triglyceride concentration decrease in the reaction capillary to the inlet triglyceride concentration.…”

Section: Enzyme Hydrolysis Of Soybean Oilmentioning

confidence: 99%

“…The developed systems are then tested for an enzyme hydrolysis of soybean oil with Thermomyces lanuginosus lipase. 20 This enzyme reaction occurs at the water-oil interface and, as we have shown recently, 21 the interface becomes unstable as the chemical compositions of both immiscible phases change during the reaction. It will be demonstrated that the three-phase (3P) slug flow arrangement provides regular separation of water-oil segments and reduces the problems coming from the interface destabilization.…”

Section: Introductionmentioning

confidence: 99%

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Three-phase slug flow in microchips can provide beneficial reaction conditions for enzyme liquid-liquid reactions

2013

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Here, we introduce a solution to low stability of a two-phase slug flow with a chemical reaction occurring at the phase interface in a microfluidic reactor where substantial merging of individual reacting slugs results in the loss of uniformity of the flow. We create a three-phase slug flow by introducing a third fluid phase into the originally two-phase liquid-liquid slug flow, which generates small two-phase liquid slugs separated by gas phase. Introduction of the third phase into our system efficiently prevents merging of slugs and provides beneficial reaction conditions, such as uniform flow pattern along the whole reaction capillary, interfacial area with good reproducibility, and intensive water-oil interface renewal. We tested the three-phase flow on an enzyme hydrolysis of soybean oil and compared the reaction conversion with those from unstable two-phase slug flows. We experimentally confirmed that the three-phase slug flow arrangement provides conversions and pressure drops comparable or even better with two-phase liquid-liquid arrangements.

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Section: F Interfacial Areamentioning

confidence: 73%

Section: F Interfacial Areamentioning

confidence: 99%

Section: B Microfluidic Systemsmentioning

confidence: 99%

Section: Enzyme Hydrolysis Of Soybean Oilmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Three-phase slug flow in microchips can provide beneficial reaction conditions for enzyme liquid-liquid reactions

2013

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Electric field assisted transport of dielectric droplets dispersed in aqueous solutions of ionic surfactants

Tuček¹,

Slouka²,

Přibyl³

2018

Electrophoresis

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Motion of liquid droplets with a surface electric charge can be efficiently controlled by dc electric field. Here, we show that the surface of a dielectric kerosene droplet can be charged by the addition of ionic surfactants to a surrounding aqueous electrolyte. The direction of droplet motion is determined by the polarity of the surfactant charge and the orientation of the imposed electric field. We have found that the effective electrophoretic mobility of dielectric droplets in a confined channel is directly proportional to the logarithm of the surfactant concentration even for values significantly exceeding critical micelle concentration (CMC). We attribute this finding not only to adsorption of ionic surfactants to the surface of dielectric droplets but also to the weakening of electro-osmosis at channel walls due to the increase of ionic strength in the aqueous phase. Our findings can be exploited in microfluidic reactors and separators for on request dosing, sampling, and separation of dielectric fluids.

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Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Onbas

Yeşil-Çeliktaş

2018

Engineering in Life Sciences

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The organic–inorganic hybrid materials have been used in different fields to immobilize biomolecules since they offer many advantages. The aim of this study was to optimize and characterize the alginate‐silica hybrid hydrogel as a stable and injectable form for microfluidic systems using internal gelation method and increase the stability and activity of immobilized enzyme for biocatalytic conversions as well. Characterization was carried out by scanning electron microscopy, energy dispersive spectroscopy/mapping, Brunauer–Emmett–Teller, Barrett–Joyner–Halenda, and Fourier‐transform infrared spectroscopy analyses, and the shrinkages of monoliths were evaluated. Subsequent to optimizing the enzyme concentration (40 μg), hydrolytic conversion of 4‐nitrophenyl β‐d‐glucopyranoside (pNPG) was performed to understand the behavior of the bioconversion in the microfluidic system. The yield was 94% which reached the equilibrium at 24 h indicating that the alginate‐silica gel derived microsystem overcome some drawbacks of monolithic systems. Additionally, bioconversion of Ruscus aculeatus saponins was carried out at the same setup in order to obtain aglycon part, which has pharmaceutical significance. Although pure aglycon could not be achieved, an intermediate compound was obtained based on the HPLC analysis. The developed formulation can be utilized for various life science applications.

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Enzyme hydrolysis of soybean oil in a slug flow microsystem

Cited by 29 publications

References 51 publications

Three-phase slug flow in microchips can provide beneficial reaction conditions for enzyme liquid-liquid reactions

Three-phase slug flow in microchips can provide beneficial reaction conditions for enzyme liquid-liquid reactions

Electric field assisted transport of dielectric droplets dispersed in aqueous solutions of ionic surfactants

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

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