Enzyme Histochemistry of Wound Healing

Raekallio, J

doi:10.1016/s0079-6336(70)80001-8

Cited by 69 publications

(19 citation statements)

References 390 publications

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“…Histochemistry allows us to study these vitally important processes. For example, in wound healing, histochemical methods for enzymes are able to detect even some of the very first reactions after injury [6]. In routine use, histochemistry has important applications in forensic medicine.…”

Section: Discussionmentioning

confidence: 99%

“…Qualitative histochemical techniques cannot routinely be relied on for unravelling the pathogenesis of a disease at the molecular level [11]. In this study, histochemical methods were used because they are non-destructive and make it possible to localize metabolical processes in tissues so that structure and function can be correlated [6]. This additional dimension, the structure, can be used to broaden our knowledge about normal functions and diseases of living tissues.…”

Section: Discussionmentioning

confidence: 99%

“…An established way to study metabolic changes in tissues is histochemistry on oxidative and hydrolytic enzymes. These methods have previously been used in studies on wound healing v. Remes [6] and in forensic medicine [7]. To evaluate the extent and duration of tissue damage and location of regenerative processes in veins used as arterial grafts, sequential changes in oxidative and hydrolytic enzyme activity in aortic vein grafts in rats have been studied earlier.…”

Section: Introductionmentioning

confidence: 99%

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Enzyme histochemical studies on veno-venous grafts in rats

Remes

1989

Res. Exp. Med.

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To study the extent, duration, and localization of metabolic changes in vein-to-vein grafts in rats, the sequential changes in enzymatic activity of veno-venous grafts in rats were evaluated by histochemical demonstration of the activity of two oxidoreductases (lactate dehydrogenase and succinate dehydrogenase) and two hydrolytic enzymes (adenosine triphosphatase and alkaline phosphatase). All the enzymes studies showed a decrease in staining 1 day after transplantation, the change being most pronounced for AFOS in the subendothelial layer. The recovery of staining intensity was noted after 3 days, the enzyme activity reaching the pregrafting level at 1 week. Different from the previous observations on vein-to-artery grafts, these vein-to-vein grafts showed less depression of enzyme activity during the first days after transplantation, neither did they display a continuing strong activity later on, probably due to lack of a thickening intimal layer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enzyme histochemical studies on veno-venous grafts in rats

Remes

1989

Res. Exp. Med.

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“…One might have imagined that such a localized trauma would cause a decrease in the speed of movement at the site of the wound, but the reverse is true. It is well known that in mammals there is an increase in proteolytic activity at wound sites (review: Raekallio, 1970), and conceivably slime moulds respond the same way. Ths interpretation is supported by the fact that if charcoal (Darco G-60) is added immediately after the cut is made, the slug is not affected by the cut and wraps itself around the charcoal.…”

Section: Woundingmentioning

confidence: 99%

Proteolysis and orientation in Dictyostelium slugs

Bonner

1993

Journal of General Microbiology

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It has been long known that the migrating slugs of the cellular slime moulds are highly sensitive to their environment and orient towards light and in temperature and chemical gradients. There is considerable evidence from past work that these orientations are governed by NH, which affects the rate of movement of cells within the slug with such precision that orientation to the external stimuli is achieved. In order to test this hypothesis further, various ways to alter the internal NH, concentration were devised. Substances that either increased or decreased proteolysis were applied to one side of the tip of a slug, thereby affecting its orientation. Some of the treatments strongly support the role of internally produced NH, in orientation, and all the treatments produce results that are consistent with the hypothesis.

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“…Anhand des Auftretens verschiedener Reaktionen kann das Wundalter innerhalb methodenabh~ingiger Grenzen festgesteUt werden. Fiir gr6t~ere Hautverletzungen ist eine Methode zur Altersbestimmung yon RaekaUio (1970RaekaUio ( , 1972 angegeben worden. Die friiheste Reaktion von gr6f~eren Hautverletzungen zeigt dabei die Adenosintriphosphatase.…”

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Identifizierung und Altersbestimmung von Nadelstichverletzungen in der menschlichen Haut

Gerlach

1977

Z Rechtsmed

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Examination of enzymic activities and histochemical reactions on needle pricks can be used to determine the age of micro-injuries with sufficient exactness. Well preserved skin shows patterns of enzymes which also appear with other skin injuries and which were described in detail especially by Raekallio (1970, 1972). In autolytical changes of the skin the adenosinetriphosphatase-reaction is still reliable and can be successfully estimated. If one has sufficient experience it is possible to fairly exactly estimate the age of an injury within the first hour after the injury by preparation of the adenosinetriphosphatase-reaction. If the times of injections are known one frequently succeeds in determining the time of death by exactly determining the age of the injury. In legal expertises the histochemical reactions can rightly be regarded as useful indications of a certain age of the injury. But they do not allow an exact estimate "beyond a reasonable doubt".

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Enzyme Histochemistry of Wound Healing

Cited by 69 publications

References 390 publications

Enzyme histochemical studies on veno-venous grafts in rats

Enzyme histochemical studies on veno-venous grafts in rats

Proteolysis and orientation in Dictyostelium slugs

Identifizierung und Altersbestimmung von Nadelstichverletzungen in der menschlichen Haut

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