Enzyme histochemical studies on the pathological changes in human Sertoli cells.

Abstract

Two forms of human Sertoli cell disorders were characterized enzyme histochemically from the testicular biopsy material of infertile and subfertile patients. Sertoli cell asthenia: a slight injury of the Sertoli cell with exfoliation of individual germ cells; marked by the rarefaction of reaction zones of thiamine pyrophosphatase (TPPase) and a decrease in lactate dehydrogenase (LDH). Sertoli cell insufficiency: severe Sertoli cell damage with the formation of a "puff" and a heavy exfoliation of germ cells (dislocation of Sertoli cell nucleus and cytoplasm along with the related germ cells into the lumen of seminiferous tubule); marked by a heterogeneous activity pattern of TPPase, the disappearance of LDH, maintenance of a slightly weakened activity of alkaline phosphatase, and an increase of acid phosphatase. In the case of Sertoli-cell-only syndrome, the high prismatic Sertoli cells showed strong acid phosphatase activity with scattered weak TPPase reaction, whereas the flat or cube-like Sertoli cells exhibited weak acid phosphatase activity with only one small round reaction zone of TPPase in each cell. In addition, the frequency of the occurrence of Sertoli cell asthenia, Sertoli cell insufficiency, and Sertoli-cell-only syndrome is reported, and its correlation with the andrological diseases discussed.

II. The effect of p‐nonylphenol on the fertility potential of male rats after gestational, lactational and direct exposure

“…Some manifestations of Sertoli cell toxicants are vacuolization, apical sloughing and shedding, germ cell necrosis, decreased seminiferous tubule fluid secretion, changes in disruption, quantity or biochemical properties of testicular components, and interstitial release of Sertoli cell proteins (Boekelheide, 1993;Russell, 1993). In this study the fist s i p s of p-NP as a Sertoli cell toxicant became evident in some animals as germ cell necrosis, apical sloughing, and vacuolization were demonstrated (Goslar et al, 1982).…”

Morphologische Untersuchungen der Spermatozoen bei Störungen der Motilität

“…4, 5). V. Z u o r d n u n g d e r f a r b e r i s c h e n u n d s t r u k t u r e l l e n S t o r u n g e n d e r M i t t e l s t u c k e / F l a g e l l a z u d e n p a t h o g e n e t i s c h e n S y n d r o m e n Die Dusseldorfer Klassifizierung der Formstorungen der Spermatozoen erlaubt eine Zuordnung der Formstorungen zu ihrer Entstehung, d.h. einen pathogenetischen Bezug (Hofmann und Haider -1985, Hofmann und Freundl -1986: die Uberstreckungen des Kopfsegmentes sind sertolizellbedingt (Goslar et al -1982); die Akrosomdefekte der Spermatozoen, in der Regel mit Storungen der Chromatinkondensierung einhergehend, sind dagegen in erster Linie leydigzellbedingt bei Hinzutreten von Storungen der Sertolizellen und der Lamina propria, in der monosymptomatischen Auspragung auch durch Storungen der basalen Geschlechtszellen (Spermatogonien, I-Spermatozyten Akrosomdefekte Spermatozoen treten in einem hoheren Anteil als die schweren ,,Uberstreckungsstorungen" der Spermatozoen in das Zervixsekret uber . Umgekehrt verhalt sich jedoch die ,,Fertilisierungs-Kapazitat" gemessen an der heterologen Oozytenpenetration (Hamster-Penetrationstest): hier liegt die starkste Einschrankung bei den akrosomdefekten Formen (Heywinkel -1985).…”