Enzyme-free and sensitive method for single-stranded nucleic acid detection based on CHA and HCR

Chen, Xiaolong; Huang, Chaowang; Nie, Fuping; Hu, Mingdong

doi:10.1039/d3ay00975k

Cited by 2 publications

(1 citation statement)

References 28 publications

(32 reference statements)

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“…CHA has found widespread use in exosome detection. 101 For example, Zhang et al introduced a durable electrochemical biosensor for the analysis of exosomal miRNA, utilizing multifunctional DNA tetrahedrons assisted catalytic hairpin assembly (MDTs-CHA). (Figure 8A).…”

Section: Cha Based Signal Amplification For Exosome Detectionmentioning

confidence: 99%

Exosomes as Powerful Biomarkers in Cancer: Recent Advances in Isolation and Detection Techniques

Zhang,

Wang,

Liu

et al. 2024

IJN

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Exosomes, small extracellular vesicles derived from cells, are known to carry important bioactive molecules such as proteins, nucleic acids, and lipids. These bioactive components play crucial roles in cell signaling, immune response, and tumor metastasis, making exosomes potential diagnostic biomarkers for various diseases. However, current methods for detecting tumor exosomes face scientific challenges including low sensitivity, poor specificity, complicated procedures, and high costs. It is essential to surmount these obstacles to enhance the precision and dependability of diagnostics that rely on exosomes. Merging DNA signal amplification techniques with the signal boosting capabilities of nanomaterials presents an encouraging strategy to overcome these constraints and improve exosome detection. This article highlights the use of DNA signal amplification technology and nanomaterials' signal enhancement effect to improve the detection of exosomes. This review seeks to offer valuable perspectives for the enhancement of amplification methods applied in practical cancer diagnosis and prognosis by providing an overview of how these novel technologies are utilized in exosome-based diagnostic procedures.

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Section: Cha Based Signal Amplification For Exosome Detectionmentioning

confidence: 99%

Exosomes as Powerful Biomarkers in Cancer: Recent Advances in Isolation and Detection Techniques

Zhang,

Wang,

Liu

et al. 2024

IJN

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Integration of RCA-Based DNA Nanoscaffold with Target Triggered RNA-Cleaving DNAzyme for Sensitive Detection of miRNA21

Li,

Lv,

Jiang

et al. 2024

Appl Biochem Biotechnol

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Enzyme-free and sensitive method for single-stranded nucleic acid detection based on CHA and HCR

Abstract: Simple, rapid, and highly sensitive methods for single-stranded nucleic acid detection are of great significance in clinical testing. While common methods are inseparable from the participation of enzymes, which greatly...

Cited by 2 publications

References 28 publications

Exosomes as Powerful Biomarkers in Cancer: Recent Advances in Isolation and Detection Techniques

Exosomes as Powerful Biomarkers in Cancer: Recent Advances in Isolation and Detection Techniques

Integration of RCA-Based DNA Nanoscaffold with Target Triggered RNA-Cleaving DNAzyme for Sensitive Detection of miRNA21

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