Enzyme-free amplified detection of miRNA based on target-catalyzed hairpin assembly and DNA-stabilized fluorescent silver nanoclusters

Gu, Jinqing; Qiao, Zhenzhen; He, Xiaoxiao; Yu, Yantuan; Lei, Yanli; Tang, Jinlu; Shi, Hui; He, Dinggeng; Wang, Kemin

doi:10.1039/d0an00545b

Cited by 31 publications

(12 citation statements)

References 31 publications

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“…The calibration curve obtained under the optimized conditions obeyed a double rectangular hyperbola equation with the formula ( R 2 0.9979), where a , b , c , and d were 2.03 × 10 6 , 0.12, 5.86 × 10 7 , and 365, respectively, and may be used to calculate the miRNA-141 concentration in the samples of interest. The curves with similar behaviors were previously reported for assays coupled with CHA/mCHA. ,, The analysis of the calibration curve showed that when the target concentration changed from 1 to 100 pM, the chemiluminescence intensity increased by 8 times, while in many of the miRNA assays reported previously, the signal value changed only by 3–4 times with changing the analyte concentration by 6–7 orders of magnitude. − …”

Section: Results and Discussionsupporting

confidence: 80%

“…The curves with similar behaviors were previously reported for assays coupled with CHA/ mCHA. 28,32,33 The analysis of the calibration curve showed that when the target concentration changed from 1 to 100 pM, the chemiluminescence intensity increased by 8 times, while in many of the miRNA assays reported previously, the signal value changed only by 3−4 times with changing the analyte concentration by 6−7 orders of magnitude. 34−38 Study of Assay Specificity.…”

Section: ■ Results and Discussionmentioning

confidence: 88%

“…In other words, the assay specificity was high. It should be noted that many miRNA assays developed with the use of CHA/mCHA also showed high specificity. ,, Interestingly, the variation of conditions of HP annealing did not affect the specificity of the assay (data not shown).…”

Section: Results and Discussionmentioning

confidence: 92%

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Improving the Sensitivity of the miRNA Assay Coupled with the Mismatched Catalytic Hairpin Assembly Reaction by Optimization of Hairpin Annealing Conditions

2021

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The mismatched catalytic hairpin assembly (mCHA), a programmable oligonucleotide circuit, is one of the promising isothermal amplification methods used in nucleic acid detection. Its limitations are related to a high background noise observed due to the target-independent hybridization of the reacting hairpins (HPs). In this work, it was shown that the introduction of salts such as NaCl and MgCl2 to HP1/HP2 annealing solutions sharply reduces the background in mCHA and simultaneously increases the signal-to-background (S/B) ratio. A comparison of the salts demonstrated the higher activity of MgCl2 as compared to NaCl. A similar effect of reducing the background was observed with a decrease in the concentration of H1/H2 probes in annealing solutions. Using the favorable annealing conditions allowed the development of an ultrasensitive chemiluminescence assay coupled with mCHA for miRNA quantitation. Except mCHA, the use of a streptavidin–polyHRP conjugate and an enhanced chemiluminescence reaction additionally increased the assay sensitivity. Notably, the optimization of the HP annealing diminished the detection limit of the assay by 2 orders of magnitude and increased the sensitivity and precision of miRNA-141 determination. The discovered fact of reducing the background by the variation of HP annealing conditions may be valuable not only for the mCHA performance but also likely for other HP-based biochemical methods.

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Section: Results and Discussionsupporting

confidence: 80%

Section: ■ Results and Discussionmentioning

confidence: 88%

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Improving the Sensitivity of the miRNA Assay Coupled with the Mismatched Catalytic Hairpin Assembly Reaction by Optimization of Hairpin Annealing Conditions

2021

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“…The finding suggests that high-quality nursing intervention could effectively boost the patients' self-care ability, help the patients to enhance their tolerance to treatment, and thus improve the patients' QOL [ 17 ]. Additionally, adverse reactions in the early stage of chemotherapy, such as nausea, vomiting, constipation, diarrhea, hair loss, and other physical discomfort, can induce a greater psychological burden such as fear and resistance in patients and can also cause patients to experience negative world-weary emotions, leading to resistance to chemotherapy and hobbling the development of subsequent treatment [ 18 , 19 ]. Consequently, there is an urgent need to comfort the patient, and high-quality nursing provides psychological comfort and psychological support in response to patients' adverse reactions.…”

Section: Discussionmentioning

confidence: 99%

Effect of High-Quality Nursing Intervention on the Quality of Life and Psychological State of Tumor Patients Undergoing First Chemotherapy

Ding

Jiang

2022

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the effect of high-quality nursing intervention on the quality of life (QOL) and psychological state of patients undergoing chemotherapy for the first time. A total of 100 malignant tumor patients admitted to Jiangyin Hospital of Traditional Chinese Medicine to receive chemotherapy for the first time from October 2018 to July 2020 were selected and randomized either to the control group or to the study group (50 cases in each group) via the random number table method. The control group received routine nursing, while the study group received high-quality nursing intervention. There was no striking difference in self-rating anxiety scale (SAS), self-rating depression scale (SDS), Karnofsky score (KPS), Eastern Cooperative Oncology Group (ECOG), and QOL between the two groups before nursing intervention (all P > 0.05 ). After nursing intervention, the SAS, SDS, KPS, ECOG, and QOL in the study group were better than those in the control group (all P < 0.05 ). The adverse reaction scores of the patients in the study group were lower than those in the control group ( P < 0.05 ). High-quality nursing intervention can effectively reduce the psychological pressure of the first chemotherapy for patients with malignant tumor, ameliorate the psychological burden of patients, relieve patients’ anxiety and fear, thus improve the chemotherapy effect, and contribute to improve their QOL.

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“…Aptamers are single-stranded nucleic acids with unique secondary structures, which could selectively bind target molecules, such as small molecules, ions, proteins, and cells . For example, a T–T mismatch can selectively capture Hg 2+ to form T-Hg 2+ -T. ,− Up to now, researchers have developed many signal amplification strategies for aptamer-based detection, including hybridization chain reaction, − nuclease amplification, rolling circle amplification, catalyzed hairpin assembly amplification, , and nanomaterial-based amplification. , These strategies need a dedicated design of DNA sequences, involve various types of reagents, and require complex assay processes.…”

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confidence: 99%

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

et al. 2021

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Increasing the local concentration of DNA-based probes is a convenient way to improve the sensitivity of biosensors. Instead of using organic solvents or ionic liquids that phase-separate with water based on hydrophobic interactions, we herein studied a classic aqueous two-phase system (ATPS) comprising polyethylene glycol (PEG) and dextran. Polymers of higher molecular weights and higher concentrations favored phase separation. DNA oligonucleotides are selectively enriched in the dextran-rich phase unless the pH was increased to 12. A higher volume ratio of PEG-to-dextran and a higher concentration of PEG also enrich more DNA probes in the dextran-rich phase. The partition efficiency of the T15 DNA was enriched around seven times in the dextran phase when the volume ratio of dextran and PEG reached 1:10. The detection of limit improved by 3.6-fold in a molecular beacon-based DNA detection system with the ATPS. The ATPS also increased the sensitivity for the detection of Hg2+ and adenosine triphosphate, although these target molecules alone distributed equally in the two phases. This work demonstrates a simple method using water soluble polymers to improve biosensors.

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Enzyme-free amplified detection of miRNA based on target-catalyzed hairpin assembly and DNA-stabilized fluorescent silver nanoclusters

Abstract: A simple, cost-effective, sensitive, and selective strategy was developed for microRNA analysis using target-catalyzed hairpin assembly and fluorescent silver nanoclusters.

Cited by 31 publications

References 31 publications

Improving the Sensitivity of the miRNA Assay Coupled with the Mismatched Catalytic Hairpin Assembly Reaction by Optimization of Hairpin Annealing Conditions

Improving the Sensitivity of the miRNA Assay Coupled with the Mismatched Catalytic Hairpin Assembly Reaction by Optimization of Hairpin Annealing Conditions

Effect of High-Quality Nursing Intervention on the Quality of Life and Psychological State of Tumor Patients Undergoing First Chemotherapy

Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System

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