Enzyme‐Entrapping behaviors in alginate fibers and their papers

Kobayashi, Yoshinari; Matsuo, Ryukichi; Ohya, Takaichi; Yokoi, Nobumasa

doi:10.1002/bit.260300316

Cited by 18 publications

(6 citation statements)

References 20 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kobayashi et al 45 used alginate fibers for enzyme immobilization. They spun an aqueous mixture of sodium alginate and enzymes into divalent metallic ion solution as a coagulating bath to produce enzyme‐containing fibers.…”

Section: Production Of Other Novel Alginate Fibersmentioning

confidence: 99%

Alginate fibres: an overview of the production processes and applications in wound management

Qin

2007

Polymer International

357

178

View full text Add to dashboard Cite

Alginate fibers are made from sodium alginate, which is a natural polymer extracted from brown seaweeds. Over the last two decades, alginate fibers have become well established in the wound management industry where their ion-exchange and gel-forming abilities are particularly useful for the treatment of exuding wounds. In order to deliver functional performances for advanced wound management products, many improvements have been made in recent years to enhance the absorption and gel-forming capabilities and the anti-microbial properties of alginate fibers. In addition, attempts have been made to use alginate fibers as a carrier to deliver zinc, silver and other active ingredients that are beneficial to wound healing. This paper reviews the development in the production of various fibers from alginate, and summarizes the production processes for calcium alginate, calcium/sodium alginate, sodium alginate, zinc alginate, silver alginate and other types of alginate fibers containing novel functional ingredients.

show abstract

Section: Production Of Other Novel Alginate Fibersmentioning

confidence: 99%

Alginate fibres: an overview of the production processes and applications in wound management

Qin

2007

Polymer International

357

178

View full text Add to dashboard Cite

show abstract

“…Schmid 1989) or the multiple use of the enzyme by its immobilization may be done. Several authors (Kobayashi et al 1987; Crump and Rozzell 1988;Yang and Su 1989) have already demonstrated the successful entrapment or covalent coupling of Bacillus macerans CGTase and its suitability for continuous CD production.…”

Section: Introductionmentioning

confidence: 99%

Production and immobilization of a proteinase-reduced cyclodextrin glycosyltransferase preparation

Steighardt

Kleine

1993

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Cyclodextrin glycosyltransferase (CGTase) was produced by a 3-day cultivation of Bacillus macerans growing in a natural medium containing grated-potatoes. Besides CGTase the culture supernatant contained a mixture of serine proteinases with a predominant subtilisin-like activity. By fractionated precipitation with ammonium sulphate the CGTase (molecular mass approximately 70 kDa) was concentrated and largely separated from the proteinases (molecular mass approximately 28 kDa). Among the various immobilization methods and carrier materials tested the enriched CGTase was covalently bound preferably onto porous glass beads using glutardialdehyde as cross-linker. The discontinuous conversion of soluble starch into beta-cyclodextrin was carried out with native as well as immobilized CGTase over 24 h. The batch re-usability of the fixed enzyme proved to be at least 20 times with a residual CGTase activity of 65%.

show abstract

“…Thus, either a mild fiber fabrication procedure or a robust enzyme is required, which restricts the selection of raw materials. Chemical functional groups on the supports are not essential for immobilization, but they can either be used to facilitate the immobilization procedure through enzyme–matrix interactions, or to adjust the porosity of the fibrous membrane [ 8 , 67 , 136 , 214 ].…”

Section: Fibrous Membrane Immobilization Supportsmentioning

confidence: 99%

“…Diverse forms of silica or silica-based particles synthesized by the sol-gel method [ 8 , 127 , 160 ] or water-soluble polymers [ 29 , 67 , 136 , 137 , 162 , 171 , 214 ] are commonly used as enzyme incorporation matrices. These polymers have the potential for being processed into fibrous membranes [ 98 , 172 , 173 , 187 ].…”

Section: Fibrous Membrane Immobilization Supportsmentioning

confidence: 99%

Developing Enzyme Immobilization with Fibrous Membranes: Longevity and Characterization Considerations

2023

View full text Add to dashboard Cite

Fibrous membranes offer broad opportunities to deploy immobilized enzymes in new reactor and application designs, including multiphase continuous flow-through reactions. Enzyme immobilization is a technology strategy that simplifies the separation of otherwise soluble catalytic proteins from liquid reaction media and imparts stabilization and performance enhancement. Flexible immobilization matrices made from fibers have versatile physical attributes, such as high surface area, light weight, and controllable porosity, which give them membrane-like characteristics, while simultaneously providing good mechanical properties for creating functional filters, sensors, scaffolds, and other interface-active biocatalytic materials. This review examines immobilization strategies for enzymes on fibrous membrane-like polymeric supports involving all three fundamental mechanisms of post-immobilization, incorporation, and coating. Post-immobilization offers an infinite selection of matrix materials, but may encounter loading and durability issues, while incorporation offers longevity but has more limited material options and may present mass transfer obstacles. Coating techniques on fibrous materials at different geometric scales are a growing trend in making membranes that integrate biocatalytic functionality with versatile physical supports. Biocatalytic performance parameters and characterization techniques for immobilized enzymes are described, including several emerging techniques of special relevance for fibrous immobilized enzymes. Diverse application examples from the literature, focusing on fibrous matrices, are summarized, and biocatalyst longevity is emphasized as a critical performance parameter that needs increased attention to advance concepts from lab scale to broader utilization. This consolidation of fabrication, performance measurement, and characterization techniques, with guiding examples highlighted, is intended to inspire future innovations in enzyme immobilization with fibrous membranes and expand their uses in novel reactors and processes.

show abstract

Enzyme‐Entrapping behaviors in alginate fibers and their papers

Cited by 18 publications

References 20 publications

Alginate fibres: an overview of the production processes and applications in wound management

Alginate fibres: an overview of the production processes and applications in wound management

Production and immobilization of a proteinase-reduced cyclodextrin glycosyltransferase preparation

Developing Enzyme Immobilization with Fibrous Membranes: Longevity and Characterization Considerations

Contact Info

Product

Resources

About