Enzyme Electrode Biosensors: Theory and Applications

Kauffmann, Jean‐Michel; Guilbault, G. G.

doi:10.1002/9780470110577.ch3

Cited by 26 publications

(14 citation statements)

References 288 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Potentiometric biosensors use ionselective electrodes (ISEs) and ion-sensitive field-effect transistors (ISFETs) to measure the change in electric potential due to the accumulation of ions resulting from an enzyme reaction. 26 Amperometric biosensors measure the change in electrical current (typically, in the nanoampere to microampere range) due to the oxidation/reduction process of an electroactive species. The working electrodes are usually a noble metal or a screen-printed layer covered by a bio-recognition element.…”

Section: Transducer Elementmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

View full text Add to dashboard Cite

The significance of microfluidics-based and microelectromechanical systems-based biosensors has been widely acknowledged, and many reviews have explored their potential applications in clinical diagnostics, personalized medicine, global health, drug discovery, food safety, and forensics. Because health care costs are increasing, there is an increasing need to remotely monitor the health condition of patients by point-of-care-testing. The demand for biosensors for detection of biological warfare agents has increased, and research is focused on ways of producing small portable devices that would allow fast, accurate, and on-site detection. In the past decade, the demand for rapid and accurate on-site detection of plant disease diagnosis has increased due to emerging pathogens with resistance to pesticides, increased human mobility, and regulations limiting the application of toxic chemicals to prevent spread of diseases. The portability of biosensors for onsite diagnosis is limited due to various issues, including sample preparation techniques, fluid-handling techniques, the limited lifetime of biological reagents, device packaging, integrating electronics for data collection/analysis, and the requirement of external accessories and power. Many microfluidic, electronic, and biological design strategies, such as handling liquids in biosensors without pumps/valves, the application of droplet-based microfluidics, paper-based microfluidic devices, and wireless networking capabilities for data transmission, are being explored.

show abstract

Section: Transducer Elementmentioning

confidence: 99%

Development and Applications of Portable Biosensors

2015

View full text Add to dashboard Cite

show abstract

“…Initially, enzyme electrochemistry was performed in solution, but good results were not obtained because of the adsorption and denaturation of the enzymes on the electrode surfaces and the highly irreversible electrode reactions that are related to electrode fouling (Armstrong 1990). At the same time, electrochemical enzyme biosensors were developed using enzymes immobilized in films on electrodes (Kauffmann andGuilbault 1992, Guilbault 1984), and it was demonstrated that enzymes immobilized in films retain high catalytic activity, even though mediators must be used to shuttle electrons between the enzymes and the electrode surfaces instead of direct electron transfer (DET). Although bioelectrochemistry and bioelectrocatalysis have been investigated since 1933, Table I summarizes the books that were published in this field for the last 20 years.…”

Section: Introductionmentioning

confidence: 99%

Advances in enzyme bioelectrochemistry

Pereira

Sedenho

Souza

et al. 2018

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and biocathodes for biofuel cells as well as to develop biosensors and implantable bioelectronics devices. Inside this scope, this review try to introduce and contemplate some relevant topics for enzyme bioelectrochemistry, such as the immobilization of the enzymes at electrode surfaces, the electron transfer, the bioelectrocatalysis, and new techniques conjugated with electrochemistry vising understand the kinetics and thermodynamics of redox proteins. Furthermore, examples of recent approaches in designing biosensors and biofuel developed are presented.

show abstract

“…. Following the initial development of biosensors, advances have been made in both design [17][18][19] and applications…”

mentioning

confidence: 99%

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H<sub>2</sub>O<sub>2</sub> in the Kidney

Palygin

Levchenko

Evans

et al. 2015

JoVE

View full text Add to dashboard Cite

Enzymatic microelectrode biosensors have been widely used to measure extracellular signaling in real-time. Most of their use has been limited to brain slices and neuronal cell cultures. Recently, this technology has been applied to the whole organs. Advances in sensor design have made possible the measuring of cell signaling in blood-perfused in vivo kidneys. The present protocols list the steps needed to measure ATP and H2O2 signaling in the rat kidney interstitium. Two separate sensor designs are used for the ex vivo and in vivo protocols. Both types of sensor are coated with a thin enzymatic biolayer on top of a permselectivity layer to give fast responding, sensitive and selective biosensors. The permselectivity layer protects the signal from the interferents in biological tissue, and the enzymatic layer utilizes the sequential catalytic reaction of glycerol kinase and glycerol-3-phosphate oxidase in the presence of ATP to produce H2O2. The set of sensors used for the ex vivo studies further detected analyte by oxidation of H2O2 on a platinum/iridium (Pt-Ir) wire electrode. The sensors for the in vivo studies are instead based on the reduction of H2O2 on a mediator coated gold electrode designed for blood-perfused tissue. Final concentration changes are detected by real-time amperometry followed by calibration to known concentrations of analyte. Additionally, the specificity of the amperometric signal can be confirmed by the addition of enzymes such as catalase and apyrase that break down H2O2 and ATP correspondingly. These sensors also rely heavily on accurate calibrations before and after each experiment. The following two protocols establish the study of real-time detection of ATP and H2O2 in kidney tissues, and can be further modified to extend the described method for use in other biological preparations or whole organs.

show abstract

Enzyme Electrode Biosensors: Theory and Applications

Cited by 26 publications

References 288 publications

Development and Applications of Portable Biosensors

Development and Applications of Portable Biosensors

Advances in enzyme bioelectrochemistry

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H<sub>2</sub>O<sub>2</sub> in the Kidney

Contact Info

Product

Resources

About