Enzyme-digested Colla Corii Asini (E’jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes

Xiao, Li; Mochizuki, Mai; Fan, Yumei; Nakahara, Taka; Liao, Feng

doi:10.1007/s13577-022-00694-5

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…Research has shown that donkey-hide gelatin could enhance the immune system by targeting and increasing T cells in the spleen [ 28 ]. Additionally, it has been reported that donkey-hide gelatin inhibits the inflammatory response and adipocyte dysfunction in adipose tissue induced by intestinal microbial lipopolysaccharide [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the Panax Notoginseng Ejiao Suppository in the Treatment of Patients with Ulcerative Proctitis and Its Effect on Inflammatory Response and Immune Function

Liu¹,

Sun²,

Wang³

et al. 2022

Disease Markers

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Objective. To investigate the efficacy of the Panax notoginseng Ejiao suppository in patients with ulcerative proctitis and its effect on inflammatory response and immune function. Methods. This study recruited 100 patients with ulcerative proctitis who were hospitalized to our hospital’s anorectal outpatient department between May 2015 and October 2020. They were randomly separated into either a control or a study group, with 50 cases in each. The control group received the mesalazine suppository, whereas the study group received the Panax notoginseng Ejiao suppository. Outcome measures included clinical effectiveness, inflammatory response, and immunological state of patients. Results. The total efficiency in the study group was significantly higher than that in the control group ( P = 0.019 ). The Mayo score and Baron endoscopic score between the two groups were significantly decreased after treatment, with lower results in the study group ( P < 0.05 ). The inflammatory variables were dramatically reduced following therapy, with the study group doing worse. Following treatment, the number of Th 17 cells declined dramatically in both groups, while the proportion of Treg cells increased significantly, with greater alterations of Th17 cells and Treg cells observed in the study group than those in the control group ( P < 0.05 ). The Panax notoginseng Ejiao suppository resulted in significantly shorter time lapses before symptom alleviation and a lower incidence of recurrence at 6 months after treatment versus mesalazine suppository ( P < 0.05 ). Conclusion. In patients with ulcerative proctitis, the Panax notoginseng Ejiao suppository significantly improves clinical efficacy, reduces the incidence of recurrence, mitigates inflammatory response, and improves immune function.

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Section: Discussionmentioning

confidence: 99%

Efficacy of the Panax Notoginseng Ejiao Suppository in the Treatment of Patients with Ulcerative Proctitis and Its Effect on Inflammatory Response and Immune Function

Liu¹,

Sun²,

Wang³

et al. 2022

Disease Markers

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“…Peptides derived from foods often share structural similarities with endogenous peptides, suggesting that they have the potential to exert a variety of activities ( Cunningham et al, 2017 ; Sato, 2018 ). Several studies have utilized in vitro digestion methods to produce E-Jiao peptides and have shown that these peptides have various activities, such as antioxidant, anti-inflammatory, immunomodulatory and antianemia effects ( Xiao et al, 2020 ; Cheng et al, 2021 ; Xiao et al, 2022 ; Cheng et al, 2023 ; Yu et al, 2023 ). An in vitro activity-guided fractionation study also revealed that a peptide derived from E-Jiao exhibits hematopoietic effect in mice ( Wu et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

Zhang,

Lin,

et al. 2024

Front. Pharmacol.

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Background:Equus asinus L. [Equidae; Asini Corri Colla] (donkey-hide gelatin, E-Jiao) is a traditional Chinese medicine renowned for its exceptional blood-supplementing effect. However, the specific components that contribute to its efficacy remain elusive. This study aimed to demonstrate that peptides are responsible for E-Jiao’s blood-supplementing effect and to explore the specific peptides contributing to its efficacy.Methods: The low molecular weight peptides of E-Jiao (LMEJ) were obtained using an in vitro digestion method. LMEJ and peptides in the rat bloodstream were characterized by peptidomics analysis. The blood-supplementing effect of LMEJ was assessed using blood-deficient zebrafish and mouse models. The effect of the peptides detected in rat blood was evaluated using the same zebrafish model, and network pharmacology analysis was performed to investigate the underlying mechanisms.Results: A total of 660 unique peptides were identified within LMEJ. Both E-Jiao and LMEJ significantly alleviated myelosuppression in mice but only LMEJ attenuated myelosuppression in zebrafish. After the administration of E-Jiao to rats, 67 E-Jiao-derived peptides were detected in the bloodstream, 41 of which were identical to those identified in LMEJ. Out of these 41 peptides, five were synthesized. Subsequent verification of their effects revealed that two of them were able to alleviate myelosuppression in zebrafish. Network pharmacology study suggested that E-Jiao may exert a blood-supplementing effect by regulating signaling pathways such as JAK-STAT, IL-17 and others. These results indicated that peptides are at least partially responsible for E-Jiao’s efficacy.Conclusion: This study provides a crucial foundation for further exploration of the bioactive components of E-Jiao.

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Combined use of hydrogen-rich water and enzyme-digested edible bird’s nest improves PMA/LPS-impaired wound healing in human inflammatory gingival tissue equivalents

Wang,

Shimamura,

Miwa

et al. 2024

Human Cell

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Enzyme-digested Colla Corii Asini (E’jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes

Cited by 4 publications

References 41 publications

Efficacy of the Panax Notoginseng Ejiao Suppository in the Treatment of Patients with Ulcerative Proctitis and Its Effect on Inflammatory Response and Immune Function

Efficacy of the Panax Notoginseng Ejiao Suppository in the Treatment of Patients with Ulcerative Proctitis and Its Effect on Inflammatory Response and Immune Function

Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides released in vivo

Combined use of hydrogen-rich water and enzyme-digested edible bird’s nest improves PMA/LPS-impaired wound healing in human inflammatory gingival tissue equivalents

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