Enzyme-digested Colla Corii Asini (E’jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents

Xiao, Li; Liao, Feng; Fan, Yumei; Miwa, Nobuhiko

doi:10.1007/s13577-020-00405-y

Cited by 11 publications

(4 citation statements)

References 35 publications

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“…After being cooled down to room temperature, the heated EBN mixture was pureed by a blender. Next, we digested the EBN solution by using a series of digestive enzyme mixtures (including gastro-intestinal enzymes, inorganic and organic compounds and others) according to our previous report [ 15 , 16 ]. The EBN solution first reacted with simulated gastric juice [ 15 ] at a volumetric rate of 125 (EBN solution): 1 (gastric juice) for 1 h at 37 °C on a bio-shaker.…”

Section: Methodsmentioning

confidence: 99%

Enzyme-Digested Edible Bird’s Nest (EBND) Prevents UV and arid Environment-Induced Cellular Oxidative Stress, Cell Death and DNA Damage in Human Skin Keratinocytes and Three-Dimensional Epithelium Equivalents

et al. 2023

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The aim of this study is to investigate the repressive effects of enzyme-digested edible bird’s nest (EBND) on the combination of arid environment and UV-induced intracellular oxidative stress, cell death, DNA double-strand breaks (DSBs) and inflammatory responses in human HaCaT keratinocytes and three-dimensional (3D) epithelium equivalents. An oxygen radical antioxidant capacity assay showed that EBND exhibited excellent peroxyl radical scavenging activity and significantly increased cellular antioxidant capacity in HaCaT cells. When EBND was administered to HaCaT cells and 3D epitheliums, it exhibited significant preventive effects on air-drying and UVA (Dry-UVA)-induced cell death and apoptosis. Dry-UVA markedly induced intracellular reactive oxygen species (ROS) generation in HaCaT cells and 3D epitheliums as quantified by CellROX® Green/Orange reagents. Once HaCaT cells and 3D epitheliums were pretreated with EBND, Dry-UVA-induced intracellular ROS were significantly reduced. The results from anti-γ-H2A.X antibody-based immunostaining showed that EBND significantly inhibited Dry-UVA-induced DSBs in HaCaT keratinocytes. Compared with sialic acid, EBND showed significantly better protection for both keratinocytes and 3D epitheliums against Dry-UVA-induced injuries. ELISA showed that EBND significantly suppressed UVB-induced IL-6 and TNF-α secretion. In conclusion, EBND could decrease arid environments and UV-induced harmful effects and inflammatory responses in human keratinocytes and 3D epithelium equivalents partially through its antioxidant capacity.

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Section: Methodsmentioning

confidence: 99%

Enzyme-Digested Edible Bird’s Nest (EBND) Prevents UV and arid Environment-Induced Cellular Oxidative Stress, Cell Death and DNA Damage in Human Skin Keratinocytes and Three-Dimensional Epithelium Equivalents

et al. 2023

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“…And Colla corii asini is a mixture of peptides and proteins, which are products from partial hydrolysis of collagen. (Xiao et al ., 2020a, 2020b). Collagen peptides have lots of biological activities including anti‐aging, antioxidant, wound healing and antifreeze according to previous studies (Ahmed et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

Colla corii asini–derived peptides as tyrosinase inhibitors: identification, inhibitory activity and molecular mechanism

Zhao

2022

Int J of Food Sci Tech

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The aim of the present study was to investigate the effect of peptides derived from Colla corii asini on tyrosinase. The sequences of Colla corii asini-derived peptides were identified using mass spectrometry. To characterise the potential peptides with tyrosinase inhibitory activity and clarify their molecular mechanism, both in silico digestion and molecular docking were used. In addition, the tyrosinase inhibitory activities of potential peptides in vitro were also validated. The results showed that Colla corii asini consisted of 472 peptides, and most peptides derived from collagen. Peptides DGGR, DGD, NAGE, LVGE and GSEG were released from the digestion of Colla corii asini-derived peptides and possessed potent inhibitory activity against tyrosinase with IC 50 values of 0.92, 0.50, 0.77, 0.66 and 0.69 mg mL À1 , respectively. The molecular interaction results showed that hydrogen bond and van der Waals interactions are important forces in the interaction of peptides with tyrosinase. Surface forces containing aromatic interaction, hydrogen bond, hydrophilicity and solvent-accessible surface contributed to the interaction between tyrosinase and peptides.

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“…The experiment of Li et al described that Ejiao digested by gastrointestinal enzymes could prevent cell death induced by H 2 O 2 . [ 44 ] Therefore, inhibiting oxidative stress may be the potential mechanism of WJD to improve ODI. However, because the specific mechanism is still unclear, further researches related to molecular mechanism are necessary.…”

Section: Discussionmentioning

confidence: 99%

Clinical efficacy of Wenjing decoction in the treatment of ovulatory disorder infertility: A systematic review and meta-analysis

Zuo

Liu

et al. 2022

Medicine

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Objectives: Wenjing decoction (WJD) was widely used in the treatment for ovulatory disorder infertility (ODI) in China, while its efficacy was not clearly known. In this study, we evaluated the clinical efficacy of WJD by meta-analysis.Methods: Eight electronic databases including Cochrane Library, PubMed, Embase, Web of Science, China National Knowledge Infrastructure, WanFang Data, VIP Database, and China Biology Medicine were searched for randomized controlled trials (RCTs) published from the inception of each database to July 1, 2021, of which the interventions involve WJD and clomiphene. Outcomes included clinical efficacy rate, pregnancy rate, ovulation rate, dominant follicle diameter, endometrial thickness, estradiol, folliclestimulating hormone, and luteinizing hormone. Meta-analysis and risk of bias were performed by RevMan 5.3 software.Results: Eleven RCTs including 915 patients, of which 476 in the intervention group and 439 in the control group. Meta-analysis showed that WJD was better than clomiphene for patients with ODI in terms of clinical effective rate (odds ratio [OR] = 1.22, 95% confidence interval [CI]: 1.08-1.34), pregnancy rate (OR = 1.54, 95% CI: 1.15-2.07), ovulation rate (OR = 1.34, 95% CI: 1.07-1.67), endometrial thickness (mean difference [MD] = 1.50, 95% CI: 0.90-2.10), and dominant follicle diameter (MD = 1.85, 95% CI: 0.68-3.02). The estradiol level (MD = 91.0, 95% CI: 80.3-101.88) in patients taking WJD was significantly higher than those taking clomiphene, while the follicle-stimulating hormone level (MD = −0.93, 95% CI: −1.13 to −0.72) and the luteinizing hormone level (MD = −4.41, 95% CI: −4.80 to −4.03) in patients taking WJD was significantly lower than those taking clomiphene. Our results also indicated that WJD combined with clomiphene was better than clomiphene alone for patients with ODI in terms of pregnancy rate (OR = 1.79, 95% CI: 1.37-2.35).Conclusions: WJD may be effective in the treatment of patients with ODI. Due to the quality and quantity of literature, RCT with large sample size and high quality need to be performed to verify our conclusion.

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Enzyme-digested Colla Corii Asini (E’jiao) accelerates wound healing and prevents ultraviolet A-induced collagen synthesis decline and wrinkle formation in three-dimensional skin equivalents

Cited by 11 publications

References 35 publications

Enzyme-Digested Edible Bird’s Nest (EBND) Prevents UV and arid Environment-Induced Cellular Oxidative Stress, Cell Death and DNA Damage in Human Skin Keratinocytes and Three-Dimensional Epithelium Equivalents

Enzyme-Digested Edible Bird’s Nest (EBND) Prevents UV and arid Environment-Induced Cellular Oxidative Stress, Cell Death and DNA Damage in Human Skin Keratinocytes and Three-Dimensional Epithelium Equivalents

Colla corii asini–derived peptides as tyrosinase inhibitors: identification, inhibitory activity and molecular mechanism

Clinical efficacy of Wenjing decoction in the treatment of ovulatory disorder infertility: A systematic review and meta-analysis

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