Enzyme-coated Janus nanoparticles that selectively bind cell receptors as a function of the concentration of glucose

Rucinskaite, Gabriele; Thompson, Sebastián; Paterson, Sureyya; Rica, Roberto de la

doi:10.1039/c7nr00298j

Cited by 18 publications

(19 citation statements)

References 28 publications

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“…[5,38] In addition, upon regulation of the motors' speed, cell targeting and internalization phenomena could be modulated, providing enhanced control and tunability of the drug delivery system. [39] Considerable efforts have been applied to the fabrication of micro-/nanomotors that fulfil the requirements for ideal drug delivery vehicles. [31,32,[40][41][42] Mesoporous silica, specifically the Mobil Composition of Matter No.…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐Powered Nanobots Enhance Anticancer Drug Delivery

Hortelão

Patiño

Pérez‐Jiménez

et al. 2017

Adv Funct Materials

225

273

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The use of enzyme catalysis to power of micro-and nanomotors exploiting biocompatible fuels has opened new ventures for biomedical applications such as the active transport and delivery of specific drugs to the site of interest. Here, urease powered nanomotors (nanobots) for the doxorubicin (Dox) anti-cancer drug loading, release and efficient delivery to cells are presented.These mesoporous silica-based core-shell nanobots are able to self-propel in ionic media, as confirmed by optical tracking and dynamic light scattering analysis. A four-fold increase in drug release is achieved by nanobots after 6 hours compared to their passive counterparts.2 Furthermore, the use of Dox-loaded nanobots presents an enhanced anti-cancer efficiency towards HeLa cells, which arises from a synergistic effect of the enhanced drug release and the ammonia produced at high concentrations of urea substrate. A higher content of Dox inside HeLa cells is detected after 1, 4, 6 and 24 hours incubation with active nanobots compared to passive dox-loaded nanoparticles. The improvement in drug delivery efficiency achieved by enzyme-powered nanobots may hold potential towards their use in future biomedical applications such as the substrate-triggered release of drugs in target locations.

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Section: Introductionmentioning

confidence: 99%

Enzyme‐Powered Nanobots Enhance Anticancer Drug Delivery

Hortelão

Patiño

Pérez‐Jiménez

et al. 2017

Adv Funct Materials

225

273

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“…Their simple anisotropic features make them interesting in a wide set of applications, including Pickering emulsions for nanoreactors, nanojets and nanomotors, enhanced catalysts, or just as building blocks for more complex supracrystals and molecular colloids. [ 24 ] Among biomedical applications, Janus nanoparticles have been proposed for cell‐membrane penetration, [ 25 ] biosensing, [ 26 ] or nanozymes, [ 27 ] but they can also be used for further straightforward selective biofunctionalization. From the possible Janus component nanoparticles, those made of gold and iron oxide have been proven as efficient theranostic platforms for multimodal imaging with enhanced resolution and sensitivity and a versatile multifunctionalization.…”

Section: Introductionmentioning

confidence: 99%

Janus Magnetic‐Plasmonic Nanoparticles for Magnetically Guided and Thermally Activated Cancer Therapy

Espinosa

Reguera

Curcio

et al. 2020

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Progress of thermal tumor therapies and their translation into clinical practice are limited by insufficient nanoparticle concentration to release therapeutic heating at the tumor site after systemic administration. Herein, the use of Janus magneto‐plasmonic nanoparticles, made of gold nanostars and iron oxide nanospheres, as efficient therapeutic nanoheaters whose on‐site delivery can be improved by magnetic targeting, is proposed. Single and combined magneto‐ and photo‐thermal heating properties of Janus nanoparticles render them as compelling heating elements, depending on the nanoparticle dose, magnetic lobe size, and milieu conditions. In cancer cells, a much more effective effect is observed for photothermia compared to magnetic hyperthermia, while combination of the two modalities into a magneto‐photothermal treatment results in a synergistic cytotoxic effect in vitro. The high potential of the Janus nanoparticles for magnetic guiding confirms them to be excellent nanostructures for in vivo magnetically enhanced photothermal therapy, leading to efficient tumor growth inhibition.

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“…The fabrication of such sophisticated structures is necessary in order to build micro- and nanomachines and chemical motors. To date, many chemically active colloidal systems comprise components, e.g ., inorganic catalysts and toxic fuels, that impede their application in biological systems. , It has been demonstrated that enzymes could power synthetic biocompatible micromotors and pumps. − However, until now only a few enzyme-powered micropumps have been published. − One major challenge is that the immobilization of enzymes to inorganic supports often reduces the turnover rate of the enzymes, as nonbiological surfaces cause conformational changes or hinder the diffusion of the substrate . Nevertheless, several papers report the immobilization of enzymes to inorganic particles and surfaces, − as well as the co-precipitation of enzymes and inorganic precursors to obtain enzymatically active aggregates. − Supports made from proteins generally represent a superior microenvironment for the immobilization of enzymes and at the same time permit their specific binding .…”

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confidence: 99%

Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity

et al. 2019

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Catalytically active colloids are model systems for chemical motors and active matter. It is desirable to replace the inorganic catalysts and the toxic fuels that are often used with biocompatible enzymatic reactions. However, compared to inorganic catalysts, enzyme-coated colloids tend to exhibit less activity. Here, we show that the self-assembly of genetically engineered M13 bacteriophages that bind enzymes to magnetic beads ensures high and localized enzymatic activity. These phage-decorated colloids provide a proteinaceous environment for directed enzyme immobilization. The magnetic properties of the colloidal carrier particle permit repeated enzyme recovery from a reaction solution, while the enzymatic activity is retained. Moreover, localizing the phage-based construct with a magnetic field in a microcontainer allows the enzyme–phage–colloids to function as an enzymatic micropump, where the enzymatic reaction generates a fluid flow. This system shows the fastest fluid flow reported to date by a biocompatible enzymatic micropump. In addition, it is functional in complex media including blood, where the enzyme-driven micropump can be powered at the physiological blood-urea concentrations.

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Enzyme-coated Janus nanoparticles that selectively bind cell receptors as a function of the concentration of glucose

Cited by 18 publications

References 28 publications

Enzyme‐Powered Nanobots Enhance Anticancer Drug Delivery

Enzyme‐Powered Nanobots Enhance Anticancer Drug Delivery

Janus Magnetic‐Plasmonic Nanoparticles for Magnetically Guided and Thermally Activated Cancer Therapy

Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity

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