Enzyme-catalyzed Ring-opening Polymerization of β-Butyrolactone Using PHB Depolymerase

Abstract

Poly(3-hydroxybutyrate) [P(3HB)] was prepared by the ring-opening polymerization of (R,S)-β-butyrolactone (BL) using two types of PHB depolymerase with or without substrate-binding domains (SBD) as the catalyst. The SBD lacking PHB depolymerase exhibited better catalytic activities for the ring-opening polymerization of BL.

Enzyme-Catalyzed Synthesis and Chemical Recycling of Polyesters

“…Though both types PHB depolymerases, with and without SBD, catalyzed the ring-opening polymerization of BL, the SBD-lacking PHB depolymerase exhibited better catalytic activities for the ring-opening polymerization of BL. [22] This attempt may be the first example of improvement in the enzyme activity of the PHB depolymerase for the polymerization. The PHB depolymerase from Pseudomonas lemoignei catalyzed the propyl esterification from fastest to slowest as follows: l-lactide (S,S), meso-lactide (R,S), d-lactide (R,R), d-butyrolactone (R,S), e-caprolactone, c-butyrolactone.…”

Enzymatic Synthesis of Polyesters via Ring-Opening Polymerization

“…The characteristic reactivities of the lactones were explained based on the tertiary structure model of the active site of PhaZAfa [96]. The substrate-binding domain lacking PhaZAfa showed higher polymerizabilities than PhaZAfa for the polymerization of the lactones [97]. The PHB depolymerase from Pseudomonas lemoignei also catalyzed the polymerization of ε-CL, which resulted in an 18% conversion of the monomer in 48 h at 70 • C to oligomers with an average degree of polymerization (DP) of 4.…”

Polyester hydrolytic and synthetic activity catalyzed by the medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces venezuelae SO1

“…Actually, it has been reported that microbial SCL-PHA depolymerases can also be employed for the production of esters in anhydrous media (Kumar et al 2000;Suzuki et al 2000Suzuki et al , 2001Suzuki et al , 2003. Similarly, it was recently been observed that MCL-PHA depolymerase from Pseudomonas fluorescens GK13 catalyzed ester-forming reactions in nonaqueous media by simply employing preparations of the lyophilized enzyme (Santos et al 2012).…”