Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116

Rosset, Daniela V.; Wancura, João H. C.; Ugalde, Gustavo A.; Oliveira, J. Vladimir; Tres, Marcus V.; Kuhn, Raquel C.; Jahn, Sérgío Luiz

doi:10.1007/s12010-019-02966-7

Cited by 24 publications

(16 citation statements)

References 32 publications

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“…An interesting point to note is that as is possible for immobilized enzymes, soluble lipases also enable their reuse after recovery from the reaction medium by simple decantation [87,107,111]. The enzyme being an amphiphilic molecule acting on the interface water/oil, remains concentrated, after separation by gravity, in an emulsion between a superior layer (rich in FAAE) and a clear inferior layer (rich in glycerol and water).…”

Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

“…The use of a liquid formulation of lipase modify the system mainly by the introduction of water in the reaction that promotes the TAG hydrolysis generating FFA that are not completely esterified in biodiesel, needing to be removed later. Most of the published papers that used lipases in liquid formulation to catalyze the synthesis of FAAE report AV out of specification (usually higher than 4.0 mg KOH • g −1 or 2.0 wt% [33,90,98,106,109,111] when no action is taken aiming to correct such parameter), making it necessary a post-reaction stage of polishing/purification of the biofuel.…”

Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

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Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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Enzymatic synthesis of biodiesel showed advantageous characteristics in relation to other technologies once it works under bland conditions, no generation of wastewater, no occurrence of saponifications reactions and production of a biodiesel with high quality. Although many researches still apply immobilized lipases, the high costs associated with this biocatalyst hamper the economic viability of the process. Lipases in free/soluble/liquid formulation employed to biodiesel production via hydroesterification reaction have attracted interest from researchers because they are more cost effective than the immobilized form, making the enzymatic route more competitive. In addition, soluble lipases present higher reaction rates, reducing the time required to obtain a satisfactory biodiesel yield. Despite the fact that already exist industrial plants producing biodiesel with the assistance of lipases in liquid formulation, results of researches show that the process still needs to overcome some drawbacks. This paper is a comprehensive and critical discussion on the publications where soluble lipases were applied on biodiesel synthesis, as well as the challenges that the technology faces and its current status in pilot and industrial applications.

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Section: Enzymatic Hydroesterification: Use Of Soluble Lipasesmentioning

confidence: 99%

Section: Challenges Of the Biodiesel Production Catalyzed By Soluble mentioning

confidence: 99%

Lipases in liquid formulation for biodiesel production: Current status and challenges

Wancura

Tres

Jahn

et al. 2019

Biotech and App Biochem

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“…Although many researches used immobilized lipases for hydroesterification, recently, the use of liquid lipases has gained popularity [235,[264][265][266]. The use of a liquid lipase instead of an immobilized lipase implies the presence of water in the process, thus, related to the first hydrolytic step.…”

Section: Hydroesterificationmentioning

confidence: 99%

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

et al. 2020

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Lipases are among the most utilized enzymes in biocatalysis. In many instances, the main reason for their use is their high specificity or selectivity. However, when full modification of a multifunctional and heterogeneous substrate is pursued, enzyme selectivity and specificity become a problem. This is the case of hydrolysis of oils and fats to produce free fatty acids or their alcoholysis to produce biodiesel, which can be considered cascade reactions. In these cases, to the original heterogeneity of the substrate, the presence of intermediate products, such as diglycerides or monoglycerides, can be an additional drawback. Using these heterogeneous substrates, enzyme specificity can promote that some substrates (initial substrates or intermediate products) may not be recognized as such (in the worst case scenario they may be acting as inhibitors) by the enzyme, causing yields and reaction rates to drop. To solve this situation, a mixture of lipases with different specificity, selectivity and differently affected by the reaction conditions can offer much better results than the use of a single lipase exhibiting a very high initial activity or even the best global reaction course. This mixture of lipases from different sources has been called “combilipases” and is becoming increasingly popular. They include the use of liquid lipase formulations or immobilized lipases. In some instances, the lipases have been coimmobilized. Some discussion is offered regarding the problems that this coimmobilization may give rise to, and some strategies to solve some of these problems are proposed. The use of combilipases in the future may be extended to other processes and enzymes.

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“…(1) 2.2 Avaliação do reuso de lipase e água É sabido que na rota enzimática de produção de biodiesel, o maior custo é associado ao elevado preço dos biocatalisadores (Rosset et al, 2019). Por essa razão, foi realizado uma avaliação do reuso sucessivo da fase pesada do produto de uma batelada de hidrólise de óleo de soja degomado catalisado por lipase de Pseudomonas fluorescens, composto por água, lipase, e glicerol, este último que fica mais concentrado a medida que cada reuso é feito.…”

Section: Perfil De Conversão Com O Tempounclassified

Hidrólise De Óleo De Soja Degomado Catalisado Por Lipase De Pseudomonas Fluorescens Livre: Avaliação De Reuso De Água E Enzima.

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Tardioli²

2019

Blucher Chemical Engineering Proceedings

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RESUMO-Com enormes preocupações relacionadas aos combustíveis fósseis, tanto em caráter de futura escassez quanto ambiental, o biodiesel, por ser biodegradável e renovável, prova-se uma alternativa interessante para suprir essa demanda energética. Dentre os processos para sua obtenção, o mais consolidado, conhecido como rota química, resume-se na transesterificação entre óleos e álcoois, gerando glicerol e biodiesel, na presença de catalisadores básicos, e dentre suas desvantagens, cita-se a necessidade de pré-tratamento de acidez da matéria-prima e do pH dos efluentes para descarte, reação com alta demanda de álcool e ausência total de água, dificuldade de recuperação do glicerol, altas temperaturas de operação e corrosão do sistema de operação. Por estas razões, a hidroesterificação catalisada por enzimas, consistindo na hidrólise de óleos com água, formando glicerol, água e ácidos graxos livres, em sequência da esterificação deste último com álcoois, formando água e biodiesel, é uma rota candidata ao aperfeiçoamento da produção de biodiesel, pois opera em condições moderadas de temperatura, não necessita de pré-tratamento de acidez da matéria prima, e o glicerol produzido é mais puro e de mais fácil recuperação. Este trabalho descreve o perfil de conversão da hidrólise de óleo de soja degomado catalisada por lipase de Pseudomonas fluorescens (LPF) livre, e a avaliação do reuso da água e lipase em sucessivas hidrólises realizadas no tempo de máxima conversão.

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Enzyme-Catalyzed Production of FAME by Hydroesterification of Soybean Oil Using the Novel Soluble Lipase NS 40116

Cited by 24 publications

References 32 publications

Lipases in liquid formulation for biodiesel production: Current status and challenges

Lipases in liquid formulation for biodiesel production: Current status and challenges

One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates

Hidrólise De Óleo De Soja Degomado Catalisado Por Lipase De Pseudomonas Fluorescens Livre: Avaliação De Reuso De Água E Enzima.

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