Enzyme catalysis on solid surfaces

Laurent, Nicolas; Haddoub, Rose; Flitsch, Sabine L.

doi:10.1016/j.tibtech.2008.03.003

Cited by 91 publications

(66 citation statements)

References 72 publications

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“…In literature, polymers such as dextran, PVA, and DEAE-starch were added to coat the particles for better stability, before or after the formation of iron oxide particles [80,81]. Otherwise, magnetic nanoparticles can be coated with silica containing a high coverage of silanol groups, which can easily be anchored with defined and generic surface chemistries [82,83]. As a result, magnetic nanoparticles coated with an ultrathin layer of silica would be more useful as magnetic nanocarrier [84].…”

Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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Compared to free enzymes in solution, immobilized enzymes are more robust and more resistant to environmental changes. More importantly, the heterogeneity of the immobilized enzyme systems allows an easy recovery of both enzymes and products, multiple re-use of enzymes, continuous operation of enzymatic processes, rapid termination of reactions, and greater variety of bioreactor designs. This paper is a review of the recent literatures on enzyme immobilization by various techniques, the need for immobilization and different applications in industry, covering the last two decades. The most recent papers, patents, and reviews on immobilization strategies and application are reviewed.

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Section: Magnetic Nanoparticlesmentioning

confidence: 99%

Enzyme immobilization: an update

et al. 2013

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“…Porous surfaces limit the accessibility of large molecules such as DNA carbohydrate polymers, and proteins, and hence Luarent et al (2008) have reviewed the increasing impact of solid surface supports for such applications. Yu and Liang (2009), with the aim of improving peroxidase loading, have taken advantage of the self assembly of polyelectrolyte (PE) coated polystyrene particles to construct three dimensional colloidal crystal arrays (CCA) and hollow colloidal crystal arrays (HCCA) with very large surface areas.…”

Section: Support Based Immobilisationmentioning

confidence: 99%

Advances in enzyme immobilisation

Brady

Jordaan²

2009

Biotechnol Lett

747

482

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Improvements in current strategies for carrier-based immobilisation have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self-immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.

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“…Recent reviews containing information on MALDI analysis in carbohydrate synthesis include an article on synthesis of polysaccharides via enzymatic polymerization (Kobayashi, 2007), a minireview of methods for synthesis of glycans labeled with stable isotopes (Yamaguchi, 2008) and a review of enzymecatalyzed reactions on solid surfaces (Laurent, Haddoub, & Flitsch, 2008b). Synthesis with glycosyl transferases is a popular alternative to purely chemical methods; large-scale synthesis of glycopeptides is frequently performed with a set of enzymes enabling transferases to be reused.…”

Section: Carbohydrate Synthesismentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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This review is the fifth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2008. The first section of the review covers fundamental studies, fragmentation of carbohydrate ions, use of derivatives and new software developments for analysis of carbohydrate spectra. Among newer areas of method development are glycan arrays, MALDI imaging and the use of ion mobility spectrometry. The second section of the review discusses applications of MALDI MS to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, biopharmaceuticals, glycated proteins, glycolipids, glycosides and various other natural products. There is a short section on the use of MALDI mass spectrometry for the study of enzymes involved in glycan processing and a section on the use of MALDI MS to monitor products of the chemical synthesis of carbohydrates with emphasis on carbohydrate-protein complexes and glycodendrimers. Corresponding analyses by electrospray ionization now appear to outnumber those performed by MALDI and the amount of literature makes a comprehensive review on this technique impractical. However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest.

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Enzyme catalysis on solid surfaces

Cited by 91 publications

References 72 publications

Enzyme immobilization: an update

Enzyme immobilization: an update

Advances in enzyme immobilisation

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

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