Enzyme Bioelectrochemistry in Cast Biomembrane-Like Films

Rusling, James F.

doi:10.1021/ar970254y

Cited by 529 publications

(412 citation statements)

References 55 publications

(127 reference statements)

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“…Michaelis et al [18] have prepared crystalline ZnO films by cathodic electrodeposition from aqueous solution in the presence of sodium laurylsulfate and obtained films of remarkably different morphologies. Rusling et al [19] immobilized hemoglobin on the electrode by incorporating it into surfactant film and realized direct electrochemistry of hemoglobin. Chen and Chzo [20] have found that simultaneous determination of dopamine and ascorbic acid can be made using didodecyldimethylammonium bromide filmmodified electrodes.…”

Section: Scheme 1 Structure Of Mitoxantronementioning

confidence: 99%

Surfactant modified carbon nanotube paste electrode for the sensitive determination of mitoxantrone anticancer drug

Manjunatha

2017

J. Electrochem. Sci. Eng.

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Section: Scheme 1 Structure Of Mitoxantronementioning

confidence: 99%

Surfactant modified carbon nanotube paste electrode for the sensitive determination of mitoxantrone anticancer drug

Manjunatha

2017

J. Electrochem. Sci. Eng.

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“…These redox peak pairs are characteristic of protein heme Fe III /Fe II redox couples in films. 7, 40,41,47 In general, CV peak potentials of redox proteins in thin films depend on the interactions of the proteins with the film material, the electrode material, and the identity of the protein. 7c, 47 In the present case, the different midpoint potentials for different {protein/HA} n films do not reflect redox potentials of proteins in solution, 7,47 as also observed in the previous work.…”

Section: Assembly Of {Protein/ha} N Layer-by-layer Filmsmentioning

confidence: 99%

“…7, 40,41,47 In general, CV peak potentials of redox proteins in thin films depend on the interactions of the proteins with the film material, the electrode material, and the identity of the protein. 7c, 47 In the present case, the different midpoint potentials for different {protein/HA} n films do not reflect redox potentials of proteins in solution, 7,47 as also observed in the previous work. 48 The peak currents increased with the number of protein/HA bilayers (n) up to a certain saturation value (n max ), confirming the successful assembly of the layer-by-layer films.…”

Section: Assembly Of {Protein/ha} N Layer-by-layer Filmsmentioning

confidence: 99%

Protecting Peroxidase Activity of Multilayer Enzyme-Polyion Films Using Outer Catalase Layers

Rusling

2007

J. Phys. Chem. B

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Films constructed layer-by-layer on electrodes with architecture {protein/hyaluronic acid (HA)} n containing myoglobin (Mb) or horseradish peroxidase (HRP) were protected against protein damage by H 2 O 2 by using outer catalase layers. Peroxidase activity for substrate oxidation requires activation by H 2 O 2 , but {protein/HA} n films without outer catalase layers are damaged slowly and irreversibly by H 2 O 2 . The rate and extent of damage were decreased dramatically by adding outer catalase layers to decompose H 2 O 2 . Comparative studies suggest that protection results from catalase decomposing a fraction of the H 2 O 2 as it enters the film, rather than by an in-film diffusion barrier. The outer catalase layers controlled the rate of H 2 O 2 entry into inner regions of the film, and biased the system to favor electrocatalytic peroxide reduction over enzyme damage. Catalase-protected {protein/ HA} n films had an increased linear concentration range for H 2 O 2 detection. This approach offers an effective way to protect biosensors from damage by H 2 O 2 .

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“…But the direct electron transfer of redox protein is difficult in the conventional electrode surface, so different kinds of films modified electrodes have been fabricated to provide a biocompatible microenvironment for protein to exchange the electron with the electrode. 3,4 Recently room temperature ionic liquids (RTILs) had been used in the protein electrochemistry. As a novel and attractive solvent, RTILs have many unique physicochemical properties such as high ionic conductivity, non-volatility, wide electrochemical windows and good solubility.…”

Section: Introductionmentioning

confidence: 99%

Direct electrochemistry of hemoglobin immobilized in polyvinylalcohol and clay composite film modified carbon ionic liquid electrode

Hui¹,

Gao²,

Li³

et al. 2009

J. Braz. Chem. Soc.

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Neste trabalho, líquido iônico hexaflúor-fosfato de 1-butil-3-metilimidazólio (BMIMPF 6 ), em temperatura ambiente, foi usado como ligante na fabricação de um eletrodo líquido iônico de carbono (CILE). Hemoglobina (Hb) foi imobilizada na superfície de CILE com argila e um filme composto por polivinil álcool (PVA) pelo método camada por camada. Espectros de UV-Vis e FT-IR mostraram que Hb no filme reteve as características essenciais de sua estrutura original. In this paper room temperature ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6 ) was used as binder to fabricate a carbon ionic liquid electrode (CILE). Hemoglobin (Hb) was immobilized on the surface of CILE with clay and polyvinyl alcohol (PVA) composite film by layer to layer method. UV-Vis and FT-IR spectra showed that Hb in the film retained the essential features of its native structure. Electrochemical experiments indicated that a pair of well-defined quasi-reversible redox peak was obtained in pH 7.0 Britton-Robinson (B-R) buffer solution. The reduction and oxidation peak potentials were located at −0.405 V and −0.274 V (vs. SCE) with the formal potential as −0.340 V, which was contributed to the electrochemical reaction of heme Fe(III)/Fe(II) redox couples. The results suggested that the direct electron transfer of Hb in the PVA/Clay film with the CILE was accomplished. The direct electrochemical behaviors of Hb were carefully studied with the electrochemical parameters calculated. The PVA/ Clay/Hb modified CILE gave excellent electrocatalytic ability to the reduction of H 2 O 2 and the apparent Michaelis-Menten constant (K M app ) value of Hb in the PVA/Clay film was calculated as 56.26 μmol L -1 .

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Enzyme Bioelectrochemistry in Cast Biomembrane-Like Films

Cited by 529 publications

References 55 publications

Surfactant modified carbon nanotube paste electrode for the sensitive determination of mitoxantrone anticancer drug

Surfactant modified carbon nanotube paste electrode for the sensitive determination of mitoxantrone anticancer drug

Protecting Peroxidase Activity of Multilayer Enzyme-Polyion Films Using Outer Catalase Layers

Direct electrochemistry of hemoglobin immobilized in polyvinylalcohol and clay composite film modified carbon ionic liquid electrode

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