Enzyme based phenol biosensors

Amperometric magnetobiosensors using poly(dopamine)-modified Fe3O4 magnetic nanoparticles for the detection of phenolic compounds

“…Using a neodymium magnet located at the bottom part of the GC disk, Fe 3 O 4 @pDA/HRP MNPs were magnetically captured in a reproducible way. The electrochemical detection of phenolic compounds was achieved thanks to the ability of such compounds to re-oxidized the HRP enzyme, acting as electron shuttles from the redox centre of the HRP molecules to the GCE surface in the presence of H 2 O 2 following the double displacement or "ping-pong" mechanism [40][41][42][43][44][45][46][47] .…”

“…MNPs, synthesized by co-precipitation method, were coated with poly(dopamine) film forming a core-shell polymeric-Fe 3 O 4 MNPs structure (Fe 3 O 4 @pDA MNPs) where HRP was immobilized. Although, HRP was immobilized using different approaches, few reports have been described in the literature using pDA as immobilization matrix [40][41][42][43][44][45][46][47] . The modified MNPs were characterized using different techniques such as XPS, AFM, FTIR, X-ray and the electroanalytical performance of the as prepared biosensor were evaluated against some common phenolic compounds.…”

“…In addition, traditional electrochemical sensors are very poor and the electrode surface may be fouled by insulating polymer films or by-products generated during phenolic detection 36,37 . Owing to those disadvantages, researchers have focused on the use on nanostructured and catalytic materials 31,38,39 or in enzyme based amperometric biosensors [40][41][42][43][44][45][46][47] .…”

Introductory Chapter: The Prospective of Biosensing in Environmental Monitoring

“…[16][17][18][19][20][21][22] have been developed. In addition, biosensors for the detection of phenolic compounds [23][24][25][26], pesticides [27][28][29][30], pathogens [9,[31][32][33][34][35] and drug residues [36,37] have been developed.…”

Development of Novel Electrochemical Biosensors Based on Horseradish Peroxidase for the Detection of Caffeic Acid

“…The most important peroxidase-based biosensors used for the detection of some phenolic compounds, the sensitive material, the detection technique, and the substrate (analyte) and detection limits (LOD) are all shown in Table 1. [36] Poly(Gly)/SiSG/MWCNTs/HRP Differential pulse voltammetry Dopamine 0.6 [37] Con A-HRP Amperometry Catechol Phenol o-Cresol 0.6 0.2 0.4 [38] HRP/DNA Amperometry Chlorogenic acid 0.7 [39] Poly(GMA-co-MTM)/PPy/CNT/HRP Amperometry Phenol Hydroquinone Pyrocatechol 2-Aminophenol 0.732 0.336 0.516 0.247 [40] HRP-horseradish peroxidase; Au/MPA/HRP-gold/mercaptoundecanoic acid/horseradish peroxidase; SAP/HRP/Au-GN-Spiny Au-Pt nanotubes/horseradish peroxidase/Au-graphene; HRP/DNA-horseradish peroxidase/DNA; Con A/HRP-concanavalin A/horseradish peroxidase Poly(Gly)/SiSG/MWCNTs/HRP-DA-poly (Glycine)/silica sol-gel/multiwalled carbon nanotubes/horseradish peroxidase; Poly(GMAco-MTM)/PPy/CNT/HRP-poly(glycine methacrylate-co-3-thienylmethyl methacrylate)-polypyrrole-carbon nanotube-horseradish peroxidase.…”