Enzyme association with lipidic Langmuir–Blodgett films: Interests and applications in nanobioscience

Girard-Egrot, Agnès; Godoy, Stéphanie; Blum, Loı̈c J.

doi:10.1016/j.cis.2005.04.006

Cited by 174 publications

(139 citation statements)

References 167 publications

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“…[11] Studies on the structure of lipid LB films have mostly been focused on fatty acids and fatty acid salts, [12] but for phospholipids, although more complex and less studied, their structures have been probed by a variety of techniques, including electron diffraction, [13][14][15] X-ray diffraction, [5,16] infrared spectroscopy, [17,18] scanning tunneling microscopy, [19] and atomic force microscopy. [20] Structural ultrafast dynamics have not been reported.…”

mentioning

confidence: 99%

Ultrafast Electron Crystallography of Phospholipids

Chen¹,

Seidel²,

Zewail³

2006

Angew Chem Int Ed

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We report herein our first study on the structure and dynamics of mono-and bilayer phospholipids, the essential elements of biological membranes, by using ultrafast electron crystallography (UEC). [1,2] The phospholipid was immobilized as a Langmuir-Blodgett film on hydrophilic or hydrophobic silicon substrates. From the diffraction patterns we determined that the dimyristoyl phosphatidic acid (DMPA; Figure 1) molecules were aligned with their aliphatic chains perpendicular to the substrate surface, and resolved the subunit cell -CH 2 -CH 2 -CH 2 -distance to be c 0 = 2.54 . After a femtosecond temperature jump in the substrate we observed, through changes in the position and intensity of the diffraction, structural dynamics on different time scales: a coherent, non-equilibrium expansion (wave-type) along the aliphatic chains on the picosecond timescale, as well as contraction and restructuring at longer times. Moreover, transient structural ordering was revealed on the ultrashort time scale. For this complex system, UEC allowed us to picture the atomic forces involved in structural dynamics at far-from-equilibrium configurations, whose collective and coherent effects may otherwise be obscured.The resemblance of both lipid Langmuir films at water surfaces and Langmuir-Blodgett (LB) films to naturally occurring biological membranes means they often serve as model systems for studying membrane structures and properties, [3,4] such as head-group organization and hydration, [5] phase transitions, [6] interactions with membrane proteins, [7,8] and proton diffusion, [9] besides other applications in molecular electronics [10] and biological sensors.[11] Studies on the structure of lipid LB films have mostly been focused on fatty acids and fatty acid salts, [12] but for phospholipids, although more complex and less studied, their structures have been probed by a variety of techniques, including electron diffraction, [13][14][15] X-ray diffraction, [5,16] infrared spectroscopy, [17,18] scanning tunneling microscopy, [19] and atomic force microscopy. [20] Structural ultrafast dynamics have not been reported. Ultrafast optical spectroscopy has been used to probe the dynamics of water solvation, [21] and inelastic neutron scattering has enabled the deduction of time scales of collective motions from the dispersion relationships.[22] UEC is well suited for the study of structural changes because of its high sensitivity, low damage at low electron flux, [23] and spatiotemporal resolutions. All experiments were carried out in our UEC apparatus, [2,24] in which the electrons are incident at a grazing angle of q i = 0-58 and are scattered by the lipid molecules to form the diffraction patterns in the far field. The scattered electrons are recorded with a low-noise, imageintensified, charge-coupled device (CCD) camera assembly capable of single-electron detection.[23]The change of the structure was initiated by a femtosecond infrared laser pulse (800 nm, 120 fs, 1 kHz repetition rate) focused onto the sample surface at an inciden...

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confidence: 99%

Ultrafast Electron Crystallography of Phospholipids

Chen¹,

Seidel²,

Zewail³

2006

Angew Chem Int Ed

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“…Various LB films based on lipid bilayers, unilamellar/bilamellar lipids vesicles, detergent micelles, and other biologically important molecules and structures which possess appropriate amphipathic properties have been used as biomembranes [68]. The functionalization of LB biomimetic membranes by association or incorporation of macromolecules with specific biological activities (enzymes, antibodies, or specific ligands), the possibility to deposit LB films on any type of substrate and the structural organization (highly ordered molecular array) and ultrathin thickness of LB films open new perspectives in the field of nanobiotechnology.…”

Section: Langmuir-blodgett Filmsmentioning

confidence: 99%

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

Chiono

Descrovi

Sartori

et al. 2010

Scanning Probe Microscopy in Nanoscience and Nanotechnology 2

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New-generation biomaterials are information-rich and incorporate biologically active components derived from Nature. The chapter reviews current approaches for the realization of biomimetic coatings for tissue engineering applications, via functionalization with bioactive molecules such as native long chains of extracellular matrix (ECM) proteins as well as short peptide sequences derived from intact ECM proteins. Biomimetic materials provide cues for cell-matrix interactions, favouring cell adhesion, proliferation, spreading, and differentiation. The recently developed scanning probe techniques for optical and spectroscopic characterization of surfaces are also described, providing advanced topographical imaging and sensitive force measurements. The spectroscopic imaging of surfaces at the nanoscale provides insight into the function and structure of biomimetic molecules and represents a tool for tailoring the surface design.

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“…Therefore this technique was used mostly in combination with other immobilization procedures. Indeed, phospholipi d s m a t r i c e s h a v e b e e n u s e d t o h o s t biocatalyst molecules that are then co-deposited onto the solid supports as LB films (Cabaj et al, 2010a;Cabaj et al, 2010b;Girart-Ergot et al, 2005) , as shown in Figure 2.…”

Section: Langmuir-blodgett Techniquementioning

confidence: 99%

“…In all these circumstances the convenient matrix may be required. For instance, according to Girart-Ergot et al (2005) enzyme bioactivity in mixed lipid LB films is preserved due to the lipid molecular assembly protects the enzyme, positioning the polypeptide moiety in such a way as to allow the recognition and signal events. In fact, phospholipids have been used as protecting agents for several types of material, not only for membrane cell proteins (Caseli et al, 2002) but also for polysaccharides (Pavinatto et al, 2007) and synthetic polymers (Caseli et al, 2001).…”

Section: Fig 2 Schematic Path Of Lb Techniquementioning

confidence: 99%