Enzyme-assisted extraction of stabilized chlorophyll from spinach

Özkan, Gülay; Bilek, Seda Ersus

doi:10.1016/j.foodchem.2014.12.059

Cited by 95 publications

(60 citation statements)

References 26 publications

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“…; Özkan et al . ). To establish a protocol of enzymatic water extraction of polysaccharides from Epimedium brevicornu , effects of these three enzymes on extraction yield were investigated.…”

Section: Resultsmentioning

confidence: 97%

“…() and Özkan et al . () found that the enzyme combinations have synergistic effects in extraction yields of other bioactive compounds. As a result, the combination of papain, cellulase and pectinase (α 3 β 3 γ 2 ) was adopted in the following experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, the highest yield of polysaccharides from Dictyophora indusiata was obtained with the synergism of cellulase, papain and pectolyase (Wu et al 2013). Also, Wang et al (2011) and€ Ozkan et al (2015) found that the enzyme combinations have synergistic effects in extraction yields of other bioactive compounds. As a result, the combination of papain, cellulase and pectinase (a 3 b 3 c 2 ) was adopted in the following experiments.…”

Section: Optimization Of Enzymatic Water Extraction Conditionmentioning

confidence: 93%

“…Single Factor Experiments of Enzyme Concentrations. Cellulase, pectinase and papain were commonly applied to enzymatic water extraction of bioactive principles from vegetable materials (Wang et al 2011;Wu et al 2013;€ Ozkan et al 2015). To establish a protocol of enzymatic water extraction of polysaccharides from Epimedium brevicornu, effects of these three enzymes on extraction yield were investigated.…”

Section: Optimization Of Enzymatic Water Extraction Conditionmentioning

confidence: 99%

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Enzymatic Water Extraction of Polysaccharides fromEpimedium brevicornuand Their Antioxidant Activity and Protective Effect Against DNA Damage

Yang

Zhang

Cao

et al. 2016

Journal of Food Biochemistry

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A protocol of enzymatic water extraction was developed to isolate antiradical and DNA damage inhibitory polysaccharides from Epimedium brevicornu (EbP) using single factor experiments and orthogonal test for the first time. Compared to conventinal heating extraction, enzymatic water extraction dramatically enhanced extraction yield, shortened extraction duration, reduced extractant consumption and decreased extraction temperature. Structural features of EbP were investigated by infrared spectroscopy. And some other physicochemical properties like solubility of EbP were identified. EbP obtained by enzymatic water extraction exhibited ability to scavenge DPPH• and ABTS+• radicals. Consistent with their free radical‐scavenging activities, Epimedium polysaccharides possessed protective effect against plasmid DNA damage induced by oxidative stress. In summary, enzymatic water extraction seems to be a promising technique for separating Epimedium polysaccharides, which might act as a natural antioxidant or a chemopreventive agent due to their free radical‐scavenging capacity and protective activity against DNA damage caused by oxidative stress. Practical Applications Polysaccharides are considered one of the most important bioactive constituents in the famous edible medicinal plant, Epimedium brevicornu. In the present study, enzymatic water extraction of polysaccharides from Epimedium brevicornu was optimized for further large‐scale industrial processing for the first time. Epimedium polysaccharides obtained by enzymatic water extraction have proved to possess antioxidant and DNA damage inhibitory activities, which show great potential for becoming a nutraceutical in healthy foods or even a phytopharmaceutical.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Optimization Of Enzymatic Water Extraction Conditionmentioning

confidence: 93%

Section: Optimization Of Enzymatic Water Extraction Conditionmentioning

confidence: 99%

See 2 more Smart Citations

Enzymatic Water Extraction of Polysaccharides fromEpimedium brevicornuand Their Antioxidant Activity and Protective Effect Against DNA Damage

Yang

Zhang

Cao

et al. 2016

Journal of Food Biochemistry

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“…Mean comparison of total chl / C(x+c) in stress × growth type × solvent interaction3. DiscussionChlorophyll is the main pigment involved in photosynthesis and two other pigments and carotenoids, play supporting roles in photosynthesis[26]. Methanol and ethanol compare well as extraction solvents used in chlorophyll and carotenoids evaluation from plants.…”

mentioning

confidence: 96%

Comparative Extraction Efficacy of Different Organic Solvents for Leaf Chlorophylls and Carotenoids in (<em>Portulaca oleracea</em> L.) Varying with Growth Behavior and Stress Type

Ahmadi¹,

Omidi²,

Mehrnia³

et al. 2019

Preprint

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The chlorophyll is one of the most important natural pigments used extensively in the food industry. Two important factors for the production of chlorophyll are the use of plants rich in chlorophyll and efficiency of extraction method. Present investigation was performed to compare the extraction of photosynthetic pigments by using solvents of different chemical nature. The purslane plants with different growth behavior viz. Scrollable and standing were grown under shade and sunshine stress condition. Different solvents including diethyl ether, 5% ethanol, pure acetone, 20% acetone, pure methanol and 10% methanol were used to extract chlorophyll and carotenoids from the purslane plant. The results indicated that stress, growth type and different solvents had a significant effect on the extraction of chlorophyll and carotenoids. Different trend was observed in extraction rate for chlorophylls and carotenoids. Among the solvents, pure methanol was the best for extraction of chl a. Methanol and acetone were appropriate solvents to achieve the highest amount of chlorophyll from plant tissues. Among different solvents, pure methanol for chl a, pure acetone and methanol for carotenoids were best solvent for purslane plant with a growing type scrollable of under shade.

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Mild and Efficient Extraction of Fluorescent Chlorophyll a from Spinach Leaves for Application as the Sustainable Emitter in Light‐Emitting Electrochemical Cells

Kotewicz,

Tang,

Edman

et al. 2024

ChemElectroChem

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Natural pigments are sustainable compounds that can be employed as emitters, sensors and sensitisers in optoelectronics. The most abundant pigment, chlorophyll, offers advantages of easily available and plentiful feedstock, biodegradability and non‐toxicity. However, strenuous extraction and separation limit its application on larger scale. In this work, a practically mild and scalable extraction and separation method for rapid isolation of chlorophyll a from spinach is presented. Three different stationary phases for column chromatography were evaluated, and a new solvent system was developed for the elution of chlorophyll a on a neutral alumina chromatography column. The purified product was obtained with a yield of 0.98 mg ⋅ g−1 with respect to the dry leaves. A first light‐emitting electrochemical cell (LEC) based on chlorophyll a as the emitter is reported, using the extracted chlorophyll a as the guest compound dispersed in a blend‐host matrix in a concentration of 2.5 or 5 mass %. The higher‐chlorophyll‐concentration LEC exhibits emission solely from the chlorophyll emitter, with the main emission peak located at 675 nm. The lower‐chlorophyll‐concentration LEC features two distinct emission bands, one in the red region that is originating from the chlorophyll guest and one in the blue region (main peak at 430 nm) that stems from the blend host. This combined red:blue emission can be attractive for, e. g., greenhouse applications, since it matches the action spectrum of plant photosynthesis.

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Enzyme-assisted extraction of stabilized chlorophyll from spinach

Cited by 95 publications

References 26 publications

Enzymatic Water Extraction of Polysaccharides fromEpimedium brevicornuand Their Antioxidant Activity and Protective Effect Against DNA Damage

Enzymatic Water Extraction of Polysaccharides fromEpimedium brevicornuand Their Antioxidant Activity and Protective Effect Against DNA Damage

Comparative Extraction Efficacy of Different Organic Solvents for Leaf Chlorophylls and Carotenoids in (<em>Portulaca oleracea</em> L.) Varying with Growth Behavior and Stress Type

Mild and Efficient Extraction of Fluorescent Chlorophyll a from Spinach Leaves for Application as the Sustainable Emitter in Light‐Emitting Electrochemical Cells

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