Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: characterization of the extracts and their bioactive potential

Vásquez, Valeria; Martínez, Ronny; Bernal, Claudia

doi:10.1007/s10811-018-1712-y

Cited by 76 publications

(61 citation statements)

References 34 publications

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“…A wide range of enzymes is used in this process as pepsin, cellulase, viscozyme, alcalse, and flavourzyme [23][24][25]. In a recent study, Vasquez et al [26] showed that the application of cellulase to Macrocystis pyrifera and Chondracanthus chamissoi resulted in protein extraction yields of 76.6% and 36.1%, respectively. However, the extraction time consumption exceeded 12 h. On the other hand, Okolie et al [27] compared several methods of extraction of fucoidan from Ascophyllum nodosum and showed that EAE produced lesser yield than the conventional extraction using chemicals.…”

Section: Manufacturing Process Of Seaweed Extract Biostimulant: the Mmentioning

confidence: 99%

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Boukhari

Barakate

Bouhia

et al. 2020

Plants

191

129

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The time when plant biostimulants were considered as “snake oil” is erstwhile and the skepticism regarding their agricultural benefits has significantly faded, as solid scientific evidences of their positive effects are continuously provided. Currently plant biostimulants are considered as a full-fledged class of agri-inputs and highly attractive business opportunity for major actors of the agroindustry. As the dominant category of the biostimulant segment, seaweed extracts were key in this growing renown. They are widely known as substances with the function of mitigating abiotic stress and enhancing plant productivity. Seaweed extracts are derived from the extraction of several macroalgae species, which depending on the extraction methodology lead to the production of complex mixtures of biologically active compounds. Consequently, plant responses are often inconsistent, and precisely deciphering the involved mechanism of action remains highly intricate. Recently, scientists all over the world have been interested to exploring hidden mechanism of action of these resources through the employment of multidisciplinary and high-throughput approaches, combining plant physiology, molecular biology, agronomy, and multi-omics techniques. The aim of this review is to provide fresh insights into the concept of seaweed extract (SE), through addressing the subject in newfangled standpoints based on current scientific knowledge, and taking into consideration both academic and industrial claims in concomitance with market’s requirements. The crucial extraction process as well as the effect of such products on nutrient uptake and their role in abiotic and biotic stress tolerance are scrutinized with emphasizing the involved mechanisms at the metabolic and genetic level. Additionally, some often overlooked and indirect effects of seaweed extracts, such as their influence on plant microbiome are discussed. Finally, the plausible impact of the recently approved plant biostimulant regulation on seaweed extract industry is addressed.

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Section: Manufacturing Process Of Seaweed Extract Biostimulant: the Mmentioning

confidence: 99%

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Boukhari

Barakate

Bouhia

et al. 2020

Plants

191

129

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“…This, in turn, implies that there is still a significant potential for protein extraction from the seaweed and other approaches such as for instance pressurized and supercritical fluid extraction (Herrero, Sánchez-Camargo, Cifuentes, & Ibáñez, 2015), addition of cofactors (Harnedy & FitzGerald, 2013a; Maehre, Edvinsen, Eilertsen, & Elvevoll, 2016), microwave-assisted extraction (Magnusson et al, 2019), ultrasound-assisted extraction (Bleakley & Hayes, 2017), or any combination thereof (Cermeño, Kleekayai, Amigo-Benavent, Harnedy-Rothwell, & FitzGerald, 2020), may be more suitable. Enzyme assisted extraction (EAE) is an emerging technology for seaweed protein extraction, showing great potential (Hardouin et al, 2016; Naseri, Marinho, Holdt, Bartela, & Jacobsen, 2020; Terme et al, 2020; Vásquez, Martínez, & Bernal, 2019). In a recent study, enzyme assisted extraction of E. denticulatum increased the protein yield up to 60% using Alcalase ® or Viscozyme ® (0.2% w/w) at pH 7 and room temperature (Naseri, Jacobsen, et al, 2020).…”

Section: Resultsmentioning

confidence: 99%

Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweedEucheuma denticulatum

Gregersen

Pertseva

Marcatili

et al. 2020

Preprint

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Seaweeds have a long history as a resource for polysaccharides/hydrocolloids extraction for use in the food industry due to their functionality as stabilizing agents. In addition to the carbohydrate content, seaweeds also contains a significant amount of protein, which may find application in food and feed. Here, we present a novel combination of transcriptomics, proteomics, and bioinformatics to determine the protein composition in two pilot-scale extracts from Eucheuma denticilatum (Spinosum) obtained via hot-water extraction. The extracts were characterized by qualitative and quantitative proteomics using LC-MS/MS and a de-novo transcriptome assembly for construction of a novel proteome. Using label-free, relative quantification, we were able to identify the most abundant proteins in the extracts and determined that the majority of quantified protein in the extracts (>75%) is constituted by merely three previously uncharacterized proteins. Putative subcellular localization for the quantified proteins was determined by bioinformatic prediction, and by correlating with the expected copy number from the transcriptome analysis, we determined that the extracts were highly enriched in extracellular proteins. This implies that the method predominantly extracts extracellular proteins, and thus appear ineffective for cellular disruption and subsequent release of intracellular proteins. Ultimately, this study highlight the power of quantitative proteomics as a novel tool for characterization of alternative protein sources intended for use in foods. Additionally, the study showcases the potential of proteomics for evaluation of protein extraction methods and as powerful tool in the development of an efficient extraction process.

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“…The application of targeted polysaccharide digesting enzymes such as cellulases, hemicellulases, β-glucanases and xylanases has been described as a food-grade approach to disrupt the macroalgae cell wall along with the intracellular polysaccharides [15]. While a range of enzyme preparations are commercially available, in some cases, an enzyme cocktail has also been used for this purpose [34]. However, seaweed cell wall composition can vary among phyla and species and, therefore, appropriate selection of carbohydrase(s) activity is required [8].…”

Section: Enzyme-assisted Protein Extractionmentioning

confidence: 99%

“…Electrophoresis has been routinely applied to identify and distinguish between different macroalgal proteins. This technique has been used in investigations associated with protein isolation, protein purification [34,48], and the effects of seasonality on macroalgal protein composition and content [11,49,50]. It has also been used to characterize peptides arising from the in vitro simulated gastrointestinal digestion or enzymatic hydrolysis of proteins [51], and to analyze and quantify amino acids including mycosporine-like amino acids (MAAs).…”

Section: Characterization Of Macroalgal Proteins By Electrophoresismentioning

confidence: 99%

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

et al. 2020

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Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed Seaweed (macroalgae) is considered as a sustainable bioresource rich in high-quality nutrients such as protein. Seaweed protein can be used as an alternative to other protein sources. Furthermore, these proteins are natural reservoirs of bioactive peptides (BAPs) associated with various health benefits such as antioxidant, antihypertensive, and antidiabetic activities. However, seaweed-derived BAPs remain underexploited due to challenges that arise during protein extraction from algal biomass. Coupled with this, limited proteomic information exists regarding certain seaweed species. This review highlights the current state of the art of seaweed protein extraction techniques, e.g., liquid, ultrasound, microwave, pulsed electric field, and high hydrostatic pressure assisted extraction. The review also focuses on the enzymatic hydrolysis of seaweed proteins and characterization of the resultant hydrolysates/peptides using electrophoretic and chromatographic techniques. This includes reference to methods employed for separation, fractionation, and purification of seaweed BAPs, as well as the methodologies used for identification, e.g., analysis by mass spectrometry. Furthermore, a bioinformatics or in silico approach to aid discovery of seaweed BAPs is discussed herein. Based on the information available to date, it is suggested that further research is required in this area for the development of seaweed BAPs for nutraceutical applications.

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Enzyme-assisted extraction of proteins from the seaweeds Macrocystis pyrifera and Chondracanthus chamissoi: characterization of the extracts and their bioactive potential

Cited by 76 publications

References 34 publications

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Trends in Seaweed Extract Based Biostimulants: Manufacturing Process and Beneficial Effect on Soil-Plant Systems

Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweedEucheuma denticulatum

Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed

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