Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: Improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase

Extraction of Cantharidin from Mylabris phalerata Pallas with Compound Enzyme and HPLC Analysis

“…It might be attributed to cellulose and pectinase which degraded cell wall. In fact, similar conclusion has been reported by Chen et al 8 and in-depth study was needed to investigate the adaptability in large-scale industry. …”

“…Unfortunately, the current chloroform and acid extraction methods usually lead to considerable yield loss 7 , therefore, there is an urgent demand for efficient extraction methods. Inspired by the extraction of flavonoids from Ginkgo biloba leaves using compound enzyme 8 , whereby high yield of active ingredients were achieved because cellulase and pectinase degraded cell wall, in this present study, we employed cellulose and pectinase to extract cantharidin from M. phalerata. Apart from comparison with other extraction methods, a reliable analysis method was also required.…”

Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase

“…It can alter the properties of compounds in a multitude of ways, e.g., changing the flavor or smell (Ribeiro 2011; Roitner et al 1984), improving water solubility (Chen et al 2011) or stability (Yamamoto et al 1990), or reducing skin irritation (Kurosu et al 2002). Glycosylation can additionally be used to produce attractive biosurfactants, alkyl glycosides (van Rantwijk et al 1999).…”

A new approach for obtaining trans-resveratrol from tree peony seed oil extracted residues using ionic liquid-based enzymatic hydrolysis in situ extraction

“…The method of enzyme-assisted extraction has been recently applied in target compound extraction from traditional plant materials, and has shown the characteristics of flexibility, environmental compatibility, and high efficiency [22]. A series of enzymes, including cellulase, xylanase, dextranase, β-glucosidase, and pectinase, has been used and compared for their ability to hydrolyze cell wall constituents, disintegrate the cell wall frame, enhance dissolution of intracellular substances, and improve the yield of target compounds [23,24].…”