Enzyme-assisted extraction, characterization, and in vitro antioxidant activity of polysaccharides from Potentilla anserina L.

Guo, Penghui; Chen, Hong; Ma, Jinpu; Zhang, Yuxuan; Chen, Hongfu; Wei, Ti; Gao, Dandan; Li, Jiansheng

doi:10.3389/fnut.2023.1216572

Cited by 8 publications

(1 citation statement)

References 40 publications

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“…The conditions of hot water extraction are generally the following: the ratio of solid to liquid is 1:5–1:30 g/mL, the extraction temperature is 60-100 °C, and the extraction time is 1–4 h [ 10 , 11 , 12 ]. However, other extraction methods, such as enzyme-, ultrasound-, and microwave-assisted extraction, are also becoming more popular [ 13 , 14 , 15 ]. Different extraction methods result in changes in the physicochemical attributes and structure of polysaccharides—mainly in their yield, total sugar content, uronic acid content, M w , monosaccharide composition, surface topography, and thermal stability [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Qiu,

Ou,

et al. 2024

Molecules

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The polysaccharides from Stemona tuberosa Lour, a kind of plant used in Chinese herbal medicine, have various pharmacological activities, such as anti-inflammatory and antioxidant properties. However, the effects of the extraction methods and the activity of polysaccharides from different parts are still unknown. Therefore, this study aimed to evaluate the effects of different extraction methods on the yields, chemical compositions, and bioactivity of polysaccharides extracted from different parts of Stemona tuberosa Lour. Six polysaccharides were extracted from the leaves, roots, and stems of Stemona tuberosa Lour through the use of hot water (i.e., SPS-L1, SPS-R1, and SPS-S1) and an ultrasound-assisted method (i.e., SPS-L2, SPS-R2, and SPS-S2). The results showed that the physicochemical properties, structural properties, and biological activity of the polysaccharides varied with the extraction methods and parts. SPS-R1 and SPS-R2 had higher extraction yields and total sugar contents than those of the other SPSs (SPS-L1, SPS-L2, SPS-S1, and SPS-S2). SPS-L1 had favorable antioxidant activity and the ability to downregulate MUC5AC expression. An investigation of the anti-inflammatory properties showed that SPS-R1 and SPS-R2 had greater anti-inflammatory activities, while SPS-R2 demonstrated the strongest anti-inflammatory potential. The results of this study indicated that SPS-L1 and SPS-L2, which were extracted from non-medicinal parts, may serve as potent natural antioxidants, but further study is necessary to explore their potential applications in the treatment of diseases. The positive anti-inflammatory effects of SPS-R1 and SPS-R2 in the roots may be further exploited in drugs for the treatment of inflammation.

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Section: Introductionmentioning

confidence: 99%

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Qiu,

Ou,

et al. 2024

Molecules

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Nondestructive discrimination of Potentilla anserina L. from different production areas based on near‐infrared spectroscopy

Ma,

Hai,

Zhang

et al. 2024

Phytochemical Analysis

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IntroductionThe traditional Chinese medicine (TCM) Potentilla anserina L. can use both as food and medicine. At present, the market mainly depends on experience to identify the species and determine the production areas of P. anserina. To ensure the quality of P. anserina, it is essential to improve the level of quality control.ObjectiveWe aimed to establish a rapid and nondestructive discrimination model to identify P. anserina from different production areas by near‐infrared spectroscopy.MethodsThe spectra of complete P. anserina medicinal materials and their powder of the same variety from four production areas were collected, and principal component analysis discriminant analysis and partial least squares discriminant analysis (PLS‐DA) were conducted based on different pretreatment methods and band selection methods. Then, the spectra of complete medicinal materials were converted into the spectra of medicinal powder for nondestructive identification.ResultsThe correct recognition rate (CRR) of the PLS‐DA discriminant model was the best after spectral preprocessing using autoscaling and competitive adaptive reweighted sampling for band selection. The CRRs of the calibration set and validation set were 100%, the CRRs of the external test set were 95%, 90%, 82%, and 88%, respectively, and the CRRs of the transfer external test set were 84%, 80%, 82%, and 86%, respectively.ConclusionWe realized the nondestructive and effective identification of P. anserina from different origins and laid a foundation for the industrialization and upgrading of TCM.

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Ultrasound Assisted Aqueous Two‐Phase Extraction of Polysaccharides from Ophiopogon japonicus: Process Optimization, Structure Characterization, and Antioxidant Activity

Tian,

Luo,

Yang

et al. 2024

Starch Stärke

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Ultrasound assisted aqueous two‐phase extraction (UATPE) is used to extract polysaccharides from Ophiopogon japonicus (OJPs). Experimental factors in UATPE process are optimized by response surface methodology. The optimal extraction conditions are obtained as follows: the ethanol concentration of 34%, ammonium sulfate concentration of 23%, solid‐to‐liquid ratio of 1:17 g/mL, and ultrasound power of 480 W, and the yield of OJPs is 9.41% ± 0.12%, and then the crude OJPs are further purified by different column chromatography to obtain a homogenous fraction (OJPs‐2‐SG), and identified its structure via different methods. OJPs‐2‐SG with a molecular weight of 1.25 × 105 Da is comprised of mannose (Man), galactose (Gal), glucose (Glc), and rhamnose (Rha) with a molar ratio of 5.15: 26.39: 68.72:8.41. Moreover, OJPs‐2‐SG contained α and β glycosidic bonds, and didn't showed a triple helix structure. Furthermore, OJPs‐2‐SG described irregular structures, and cylindrical and conical block like features. The IC50 values of scavenging 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), hydroxyl (OH), and superoxide (O2‐) radicals for OJPs‐2‐SG achieved 3.72 mg/mL, 3.89 mg/mL, and 8.71 mg/mL, respectively. These results provide important references for the extraction and application of polysaccharides.

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Enzyme-assisted extraction, characterization, and in vitro antioxidant activity of polysaccharides from Potentilla anserina L.

Cited by 8 publications

References 40 publications

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Study of the Structure and Bioactivity of Polysaccharides from Different Parts of Stemona tuberosa Lour

Nondestructive discrimination of Potentilla anserina L. from different production areas based on near‐infrared spectroscopy

Ultrasound Assisted Aqueous Two‐Phase Extraction of Polysaccharides from Ophiopogon japonicus: Process Optimization, Structure Characterization, and Antioxidant Activity

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