Enzyme-Assisted Aqueous Oil Extraction from High Oleic Sunflower Seeds in a Scalable Prototype Reactor

Munder, Simon; Latif, Sajid; Müller, Joachim

doi:10.1007/s12649-018-0418-3

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Such enzyme-assisted AOE methods, sometimes combined with protein extraction, have been studied and applied to different kinds of oilseeds: rapeseed [ 5 , 6 ], canola [ 7 ], sunflower [ 8 , 9 , 10 , 11 ], sesame [ 12 ], maize [ 13 ], groundnut [ 4 ], almond [ 14 ] and safflower [ 15 ], but the most studied matrix is undoubtedly soybean [ 16 , 17 , 18 , 19 , 20 ]. Many studies have reported the development of ultrasound-assisted AOE [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ], but other methods are also used, including microwave- [ 29 ], pulsed electric field-[ 9 ], pressure shockwave- [ 30 ] and surfactant-assisted [ 31 , 32 ] extraction.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Integrated Process for the Recovery of Oil Bodies or Fatty Acid Emulsions from Sunflower Seeds

et al. 2022

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An aqueous integrated process was developed to obtain several valuable products from sunflower seeds. With a high-shear rate crusher, high-pressure homogenization and centrifugation, it is possible to process 600× g of seeds in 1400× g of water to obtain a concentrated cream phase with a dry matter (dm) content of 46%, consisting of 74 (w/w dm) lipids in the form of an oil-body dispersion (droplet size d(0.5): 2.0 µm) rich in proteins (13% w/w dm, with membranous and extraneous proteins). The inclusion of an enzymatic step mediated by a lipase made possible the total hydrolysis of trigylcerides into fatty acids. The resulting cream had a slightly higher lipid concentration, a ratio lipid/water closer to 1, with a dry matter content of 57% consisting of 69% (w/w) lipids, a more complex structure, as observed on Cryo-SEM, with a droplet size slightly greater (d(0.5): 2.5 µm) than that of native oil bodies and a conserved protein concentration (12% w/w dm) but an almost vanished phospholipid content (17.1 ± 4.4 mg/g lipids compared to 144.6 ± 6 mg/g lipids in the oil-body dispersion and 1811.2 ± 122.2 mg/g lipids in the seed). The aqueous phases and pellets were also characterized, and their mineral, lipid and protein contents provide new possibilities for valorization in food or technical applications.

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Section: Introductionmentioning

confidence: 99%

Aqueous Integrated Process for the Recovery of Oil Bodies or Fatty Acid Emulsions from Sunflower Seeds

et al. 2022

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“…Moreover, the capacity of the magnetic nanobiocatalyst to operate with high activity at broad pH levels is especially advantageous for large-scale oil and protein recovery from oilseeds as it allows to maintain pH conducive for enzyme action and remote from protein isoelectric point required for releasing the oil . Previous enzymatic oilseed processing studies used various proteases with different optimal pHs (pH 7 and 8.5 for sesame, pH 6.5 for moringa, pH 6 for maize, pH 7 for soybean, pH 8 for peanut, and pH 8.2 for sunflower,) to recover maximum oil and proteins depending on the type of oilseed. Thus, the protease magnetic nanobiocatalyst prepared in the present study which shows high activity in wide pH ranges could be used as a versatile biocatalyst for oilseed processing.…”

Section: Resultsmentioning

confidence: 99%

Eco-Sustainable Oilseed Biorefinery: Integrating Microwave with Magnetic Nanobiocatalysis

Talekar

Vijayraghavan

Arora

et al. 2020

ACS Sustainable Chem. Eng.

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We introduce a recyclable and efficient enzymatic process by integration of microwave (MW) pretreatment followed by magnetic nanobiocatalysis for the green and sustainable oilseed biorefinery. The magnetic nanobiocatalyst was prepared by immobilizing protease onto iron oxide magnetic nanoparticles and characterized using Fourier transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, and transmission electron microscopy. The optimized MW pretreatment (450 W for 4 min) of fresh waste pomegranate seeds (WPS) followed by the magnetic nanobiocatalyst treatment (protease dosage of 30 U g–1 DW of WPS, pH 7, and 45 °C for 10 h) recovered 17.8% of oil slightly less than the hexane-derived oil (18.6%) together with the protein (15.9%) and dietary fiber-rich (97%) solid residue as coproducts. However, the magnetic nanobiocatalyst without MW pretreatment of WPS recovered only 13.1% of oil and 11% of protein even after 16 h of reaction. Excellent thermal stability and magnetic separation enabled the reuse of the magnetic nanobiocatalyst for at least four cycles without compromising the oil, protein, and dietary fiber recovery and protease activity. Additionally, the fatty acid composition, punicic acid and phenolic content, antioxidant activity of oil, and fiber composition and functional properties (oil/water holding capacity, glucose absorption capacity, and glucose dialysis retardation index) of the dietary fiber-rich solid residue from each cycle were also similar.

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“…Several studies have been reported regarding the evaluation of AEE in obtaining oils from various seeds such as tiger nut (Cyperus esculentus L.) [59], sacha inchi (Plukenetia volubilis L.) [60], Jatropha curcas [61], Jicaro [62], pomegranate [63], castor oil [64], Pinus pumila [25], Moringa oleifera [65], Pumpkin (Cucurbita pepo) [50], sunflower [66], soybean [67], almond [49], etc. Although the enzymatic oil extraction has been widely explored, in the reported studies, different oil yields are observed for each seed; this is due to the fact that the composition of the cell wall of the seed, which is quite heterogeneous, is different for each seed, so different enzymes and conditions are necessary in each case.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Extraction of Seed Oil from Mamey Sapote (Pouteria sapota) after Viscozyme L Treatment

et al. 2021

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In this study, aqueous enzymatic extraction (AEE) was evaluated during the process of obtaining oil from mamey sapote seed (OMSS). Viscozyme L enzyme complex was used at pH 4 and 50 °C during the optimization of the extraction process by central composite design and response surface methodology. Optimal conditions were: 3.5% (w/w) of enzyme (regarding the seed weight), 5.5 h of incubation time, 235 rpm of agitation rate, and 1:3.5 of solid-to-liquid ratio. These conditions enabled us to obtain an OMSS yield of 66%. No statistically significant differences were found in the fatty acid profile and physicochemical properties, such as the acid and iodine values and the percentage of free fatty acids, between the oil obtained by AEE or by the conventional solvent extraction (SE). However, the oxidative stability of the oil obtained by AEE (11 h) was higher than that obtained by SE (9.33 h), therefore, AEE, in addition to being an environmentally friendly method, produces a superior quality oil in terms of oxidative stability. Finally, the high oil content in mamey sapote seed, and the high percentage of oleic acid (around 50% of the total fatty acid) found in this oil, make it a useful edible vegetable oil.

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Enzyme-Assisted Aqueous Oil Extraction from High Oleic Sunflower Seeds in a Scalable Prototype Reactor

Cited by 9 publications

References 25 publications

Aqueous Integrated Process for the Recovery of Oil Bodies or Fatty Acid Emulsions from Sunflower Seeds

Aqueous Integrated Process for the Recovery of Oil Bodies or Fatty Acid Emulsions from Sunflower Seeds

Eco-Sustainable Oilseed Biorefinery: Integrating Microwave with Magnetic Nanobiocatalysis

Aqueous Extraction of Seed Oil from Mamey Sapote (Pouteria sapota) after Viscozyme L Treatment

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