Enzyme and protein mass transfer coefficient in aqueous two-phase systems—II. York-Scheibel extraction column

Jafarabad, K. Rostami; Patil, Trupti; Sawant, S.B.; Joshi, Jyeshtharaj B.

doi:10.1016/0009-2509(92)80201-m

Cited by 20 publications

(3 citation statements)

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“…It is seen that hold-up increases by increasing the velocity of continuous phase, however, the amount of this variation is not considerable. Similar results have been reported in other extraction columns including bubble columns, slurry reactors, airlift loop reactors, fluidized beds and spray extraction columns [28][29][30] . 4.…”

Section: Fig 2-effect Of Rotor Speed On the Hold-up (V C =V D = 667supporting

confidence: 89%

Hold-up and flooding characteristics in a perforated rotating disc contactor (PRDC)

et al. 2015

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Dispersed phase hold-up and flooding behavior were investigated in a pilot plant scale perforated rotating disc contactor (PRDC) using three different liquid-liquid systems. The influence of various operational parameters including rotor speed, continuous and dispersed phases flow rates on flooding velocities and hold-up have been studied. Changes in the continuous phase velocity has a minor effect on dispersed phase hold-up while increasing dispersed phase flow rate and rotor speed results in an increase in dispersed phase hold-up. Moreover, based on physical properties of liquid systems and operating conditions two empirical correlations were derived for flooding point and dispersed phase hold-up. The proposed empirical correlations are thenvalidated through comparing with experimental data. Therefore, due to lack of experimental data on this type of column, these predictive empirical correlations can be used for accurate design of perforated rotating disc contactor.

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Section: Fig 2-effect Of Rotor Speed On the Hold-up (V C =V D = 667supporting

confidence: 89%

Hold-up and flooding characteristics in a perforated rotating disc contactor (PRDC)

et al. 2015

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“…On the other hand, studies with spray columns in aqueous two-phase systems demonstrated an increase of the mass transfer coef®cient with VD increase, once the drop size at higher velocities is minor and produces high areas of mass transference [2,6,9,13,14].…”

Section: 2mentioning

confidence: 95%

Hydrodynamics and mass transfer in aqueous two-phase protein extraction using a continuous perforated rotating disc contactor

Porto

Sarubbo

Lima-Filho

et al. 2000

Bioprocess Engineering

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A continuous perforated rotating disc contactor was used to extract bovine serum albumin (BSA) with aqueous two-phase systems based on polyethylene glycol (PEG) and phosphate salts. The dispersed phase holdup and mass transfer coef®cient were determined. It was found that the dispersed phase holdup increased with increasing PEG phase velocity. The overall mass transfer coef®cient for BSA was independent of the PEG phase velocity.List of symbols C concentration of BSA in phosphate-rich phase (mg/ml) C u concentration of BSA in PEG-rich phase (mg/ml) C ui concentration of BSA in PEG-rich phase at the column inlet (mg/ml) C uo concentration of BSA in PEG-rich phase at the column outlet (mg/ml) H dispersed phase holdup K da mass transfer coef®cient (min )1 ) K partition coef®cient VD dispersed phase¯ow rate (ml/min) IntroductionAqueous two-phase (biphasic) systems have been extensively used for separation and analytical studies of biomaterials since Albertsson [1] developed the technique in the 1950s. Although similar in principle to conventional liquid-liquid extraction, aqueous two-phase extraction has uncommonly wide ranges of physical properties. For instance, the density difference between the two phases varies over the range 10±100 kg/m 3 , the interfacial tension over the range 10 )3 ±10 )2 mN/m, and the viscosities over the range 10±1000 mPa. Conventional liquid-liquid extraction has no property ranges of this magnitude [2]. It has been shown that conventional extraction techniques can be adequately applied to aqueous two-phase systems such as polymer-salt or polymer-polymer systems [1].Aqueous two-phase systems have an important potential in downstream processing such as continuous operation for the large-scale separation of biomolecules. A number of different conventional contactors, e.g. spray columns and packed columns, can be used for aqueous two-phase extraction [3]. Extraction columns with rotatory motion, namely a rotating disc contactor (RDC) and a perforated rotating disc contactor (PRDC), with free areā ow [4] have been used in industry as alternative apparatus to conventional separations equipment. With this type of extractor, high mass transfer rates between the phases were obtained to recover a recombinant cutinase from an aqueous solution to a reversed micellar phase of AOT in isooctane [5].For the design of commercial scale columns, data on the following parameters are needed: protein mass transfer coef®cients, dispersed phase holdup and the extent of mixing in both the phases. Usually, these parameters depend on the velocities of the two phases, their physical properties, the column diameter and the sparger design. In this work it is analyzed bovine serum albumin (BSA) mass transfer data from a laboratory perforated rotating disc contactor (PRDC) using the PEG-phosphates system. The BSA transfer took place from the PEG phase to the salt phase. Speci®cally, the dispersed phase holdup (H) is measured and the mass transfer coef®cients determined as well as the correlations for different disperse...

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“…Estes resultados estão em concordância com os obtidos por Pawar et ai (1997), em coluna "spray", modificada de modo a gerar maiores valores da fração retida da fase dispersa.Quanto à influência da velocidade da fase dispersa na fração retida da fase dispersa em diferentes composições dos sistemas aquosos, pode-se verificar que para os vários sistemas com PEG de diferentes massas moleculares (550, 1000, 3350 e 8000), a fração retida da fuse dispersa aumentou com o aumento da velocidade da fuse dispersa e diminuiu com o aumento da composição do sistema.Kumar e Hartland (1982), relataram que o tamanho da gota diminui e o número de gotas aumenta com o aumento da velocidade da fuse dispersa, resultando em um aumento dos valores da fração retida da fuse dispersa. Efeitos similares foram observados em outros trabalhos(Jafarabad et al, 1992 a; Pawar et ai, 1997; Venâncio et ai, 1995).…”

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Estudo e desenvolvimento de uma micro-coluna de campanulas pulsantes para a purificação de proteinas

PaulaBrescanciniRabelo¹

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ADeusAo professor Elias Basile Tambourgi, pela orientação, incentivo, amizade e confiança que estiveram sempre presentes durante todo o desenvolvimento deste trabalho.Ao professor Roberto Rodrigues de Souza pela orientação e amizade, que foram muito importantes durante a execução deste trabalho.Aos amigos do laboratório de processos de separações Douglas, Joseane, Arlan, Dorival, Sérgio e Eliana pelo companheirismo, dedicação, amizade e colaboração na execução deste trabalho.Aos alunos Dinis, Johannes, Fabíola, Anelise, Rafael, Mônica e Mônica Bim pelo incentivo e colaboração nos trabalhos experimentais.Ao professor João Alexandre Ferreira da Rocha Pereira pela ajuda no desenvolvimento do equipamento experimental e pelo interesse e incentivo sempre demonstrados durante a realização deste trabalho.À professora Teima Teixeira Franco e ao professor Pedro Volpe pela disponibilidade, atenção, ajuda e valiosas colaborações.À professora Ana Lúcia Figueiredo Porto, cuja ajuda e valiosas colaborações foram fundamentais para a realização deste trabalho. Agradeço a disponibilidade, dedicação e boa vontade que sempre foram a mim dispensados. À Rosa, ao técnico Sandro Piola, e às analistas de sistemas Andreia e Joseane, pela colaboração, boa vontade e paciência a mim dispensados.Aos professores e funcionários da Faculdade de Engenharia Quirnica pelo apoio e amizade dispensados durante a realização deste trabalho. Ao CNPq pelo suporte financeiro.A todos os amigos da Pós-Graduação e que compartilharam este periodo junto a mim, pelos momentos de companheirismo, amizade, apoio e estimulo.À minha família pelo incentivo, apoio, amizade, interesse e paciência que foram a mim dispensados.A todos os amigos que direta ou indiretamente colaboraram na realização deste trabalho.valores da fração de retenção da fase dispersa variaram entre 0,09 e 0,425. As condições de inundação não foram atingidas. Assim, o principal objetivo deste trabalho, que era o desenvolvimento de uma coluna de extração que aumentasse o tempo de contato entre as fases e a obtenção de altas taxas de transferência de massa, sem desnaturar as enzimas e proteínas, fui alcançado com sucesso.

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Enzyme and protein mass transfer coefficient in aqueous two-phase systems—II. York-Scheibel extraction column

Cited by 20 publications

References 1 publication

Hold-up and flooding characteristics in a perforated rotating disc contactor (PRDC)

Hold-up and flooding characteristics in a perforated rotating disc contactor (PRDC)

Hydrodynamics and mass transfer in aqueous two-phase protein extraction using a continuous perforated rotating disc contactor

Estudo e desenvolvimento de uma micro-coluna de campanulas pulsantes para a purificação de proteinas

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