Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering

Das, Anindita; Chakrabarti, Abhijit; Das, Puspendu K.

doi:10.1016/bs.mie.2017.01.020

Cited by 3 publications

(3 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, interaction between spectrin and HbE could be electrostatic in nature and hence stronger than HbA or HbS. Recently, adsorption of organic molecules and proteins on the surface of metal nanoparticle − has been studied using the label free SHLS in solution by using a modified Langmuir model where decrease in second harmonic signal is related to the concentration of Hb variant as, Where I SH is the normalized SH scattered light intensity, Q ′ is a quenching factor that represents the extent of quenching of the signal by the Hb variants, N max is the maximum number of binding sites per unit volume, C is the total concentration of the Hb molecules per unit volume, K is the equilibrium binding constant, and 55.5 is the molarity of water. The experimental data points were fitted by using eq for all the variants (shown in Figure a) with three adjustable parameters.…”

Section: Resultsmentioning

confidence: 99%

Protein–Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study

2017

Self Cite

View full text Add to dashboard Cite

In this article, we have studied the binding of different naturally occurring hemoglobin (Hb) variants on erythrocyte skeletal protein, spectrin surface using the label free nondestructive second harmonic light scattering (SHLS) technique in aqueous buffer. Hemoglobin variants like sickle hemoglobin (HbS) and hemoglobin E (HbE) were chosen as they associate with sickle cell disease and HbEβ-thalassemia, respectively, and their interaction with spectrin is compared with normal adult hemoglobin (HbA). The concentration dependent change in the second harmonic light intensity from nanomolar spectrin solution has been measured after addition of small aliquots of hemoglobins. From the second harmonic titration data, the binding constant is calculated using a modified Langmuir adsorption model of hemoglobin binding to the spectrin surface. Interestingly, it is found that the binding constant for HbE (13.8 × 10 M) is 1 order of magnitude higher than that of HbS (1.6 × 10 M) or HbA (2.1 × 10 M) which indicates higher affinity of HbE for spectrin compared to HbA and HbS. The number of the Hb molecules bound to the spectrin surface was estimated to be of the order of hundred's which is determined for the first time.

show abstract

Section: Resultsmentioning

confidence: 99%

Protein–Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although fluorescence spectroscopy and ITC are widely used for investigating nanoparticle-protein binding studies, 10 these techniques have several drawbacks. In ITC measurements, the Wiseman C parameter, defined as C = [M] tot K a where [M] tot is total macromolecular concentration and K a is the binding constant representing the strength of interactions, 11 should be Z 10 for good signal to noise ratio. Determination of the thermodynamic parameter for an interacting system with C Z 10, high nanoparticle concentration and 1 : 1 binding stoichiometry is required, which is difficult to achieve in all cases.…”

Section: Introductionmentioning

confidence: 99%

“…Determination of the thermodynamic parameter for an interacting system with C Z 10, high nanoparticle concentration and 1 : 1 binding stoichiometry is required, which is difficult to achieve in all cases. 11,12 Thus, alternative techniques for investigating nanoparticle-protein interaction are very much required to get rid of many drawbacks of popularly used techniques.…”

Section: Introductionmentioning

confidence: 99%

Lysozyme adsorption on carbonaceous nanoparticles probed by second harmonic light scattering

Saikia,

Dutta,

Mishra

et al. 2023

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

show abstract

Second harmonic scattering multipole analysis of ligand-decorated gold nanoparticles

Zhang¹,

Lu²,

Li³

et al. 2022

Acta Phys. Sin.

View full text Add to dashboard Cite

Ligand decoration of noble metallic nanoparticles is often needed for some applications, such as biochemical sensing, catalysis and nanotechnology, and the understanding of its process is of great importance. The second harmonic scattering (SHS) technique with advantages of surface-sensitivity and label-free detection, provides intrinsic information for such a research. In this work, the second harmonic(SH) scattering patterns of two types of ligands (cetyltrimethylammonium chloride and L-cysteine) capped gold nanoparticles (GNPs) with the same radii are measured. Both the intensities and shapes of the SH scattering patterns are changed after the ligand exchange process. In order to explain the pattern changes, the analytic expressions of SH scattering are derived theoretically for a relatively large nanoparticle based on Dadap’s multipolar theory. Considering the derived relationship between the multipole (up to octopole) contributions and the power of the nanosphere radius, the effective size effect is introduced to express the SH scattering signal change for different ligand decorations and well explain the experimental results. This theory provides a new perspective of the SH scattering response to different capping ligands and offers a possible quantitative method to analyze interface physical chemistry for ligands on the surface of nanoparticles.

show abstract

Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering

Cited by 3 publications

References 90 publications

Protein–Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study

Protein–Protein Interaction Probed by Label-free Second Harmonic Light Scattering: Hemoglobin Adsorption on Spectrin Surface as a Case Study

Lysozyme adsorption on carbonaceous nanoparticles probed by second harmonic light scattering

Second harmonic scattering multipole analysis of ligand-decorated gold nanoparticles

Contact Info

Product

Resources

About