Enzyme Activity Determinations in Bone and Cartilage

Abstract

The data on bone and cartilage enzymology available in the literature and gained by our own experiments were reviewed and discussed in respect to their metabolic significance in bone and cartilage cell metabolism. Enzyme distribution patterns of main metabolic pathway enzymes reflect the metabolic features of these tissues suggesting a predominant anaerobic energy and intermediary metabolism. Enzyme activities of matrix components synthesizing enzymes as well as degrading enzymes are responsible for the maintainance of the biological steady state of the macromolecular matrix components which enables bone and cartilage tissue to perform their specific biological functions.

Effect of Low Oxygen Tensions on Glucose-Metabolizing Enzymes in Cultured Articular Chondrocytes

“…Glycolytic activity characteristically is higher in cultured cells than in vivo (14). I t is thus not surprising that the enzyme pattern of the cultured fibrocytes, as well as the chondrocytes, resembled intact articular cartilage ( 15). Nevertheless, the significantly higher LDH:MDH ratio of the cultured chondrocytes is consistent with preservation of a chondroid phenotype in monolayer culture.…”

Enzyme Activities in Chick Embryonic Cartilage

“…The distribution patterns for mitochondrial, lysosomal, microsomal, and soluble enzymes obtained are basically similar to those found in a prototype tissue, mammalian liver, although some qualitative and quantitative differences were observed . While this work was in progress, two reports on the enzymatic composition of cartilage appeared (13,14) . The present report deals with some enzymes which have not been examined in detail thus far in their intracellular localization .…”