Enzyme activities in fish spermatozoa with focus on lactate dehydrogenase isoenzymes from herring Clupea harengus

Gronczewska, Jadwiga; Ziętara, Marek S.; Biegniewska, Anna; Skorkowski, Edward F.

doi:10.1016/s1096-4959(02)00192-6

Cited by 23 publications

(14 citation statements)

References 20 publications

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“…There are two LDH isoenzymes in herring spermatozoa, LDH-B 4 and LDH-A 2 B 2 (Gronczewska et al 2003). The presence of both the LDH-B 4 and LDH-A 2 B 2 isoforms normally represents the best combination for aerobic conditions and confirms the findings of Lahnsteiner et al (1999).…”

supporting

confidence: 60%

“…Very little information is available on CK activity in fish spermatozoa as compared to what is known on its activity and gene expression in somatic tissues (Grzyb et al 2003). Assays of CK activity in spermatozoa from fish, measured under identical conditions, showed that CK activity in both carp and catfish spermatozoa is relatively high and essentially similar (Gronczewska et al 2003;Rurangwa et al 2002). Thus, it is unlikely that CK activity alone is responsible for differences in the rate of ATP depletion between catfish and carp spermatozoa.…”

mentioning

confidence: 99%

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Bioenergetics of fish spermatozoa during semen storage

Ziętara

Biegniewska

Rurangwa

et al. 2009

Fish Physiol Biochem

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This mini-review focuses on changes in ATP and creatine phosphate concentrations in fish sperm under storage conditions. The storage of catfish sperm at 4 degrees C leads to ATP depletion and decreased sperm motility. The rate of intracellular ATP depletion can be diminished through the addition of energetic substrates to the sperm storage medium, with lactate + pyruvate being the most efficient substrates for maintaining ATP concentrations in catfish sperm. The decrease in ATP concentration is closely associated with increases in AMP and hypoxanthine content. In contrast to catfish sperm, carp sperm is able to maintain intracellular ATP concentration close to the physiological level during storage. Collectively, these results suggest that fish species differ in terms of the energy metabolism of their spermatozoa and that the semen storage medium must be carefully selected for a particular fish species so as to maintain the ATP concentration and adenylate energy charge close to physiological values as long as possible.

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supporting

confidence: 60%

mentioning

confidence: 99%

Bioenergetics of fish spermatozoa during semen storage

Ziętara

Biegniewska

Rurangwa

et al. 2009

Fish Physiol Biochem

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“…Additionally, a long-life increase production of LDH in cells is expected under anaerobic conditions until they become necrotic. Studies show that isozymes in carp are similar to subunit types in mammals (Gronczewska et al 2003). Nadal-Ginard (1978) determined that the half-life of isozymes in mammals was related to tissue type, with a half-life of 4.3 days found in the liver, indicating that the rate of degradation plays an important role in enzyme concentration.…”

Section: Resultsmentioning

confidence: 99%

Biomarkers of Cytotoxic, Genotoxic and Apoptotic Effects in Cyprinus carpio Exposed to Complex Mixture of Contaminants from Hospital Effluents

Olvera-Nestor

Morales-Ávila

Gómez-Oliván

et al. 2016

Bull Environ Contam Toxicol

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Hospital wastewater is an important source of emerging contaminants. Recent studies emphasize the importance of assessing the effects of mixtures of contaminants rather than environmental risk of their individual components, as well as the determination of intrinsic toxicity of wastewater. Mixtures of pollutants has possible interactions that have notable environmental side effects. The aim of this study is an attempt to characterize biomarkers in Cyprinus carpio related to the exposure to a complex mixture of contaminants found in hospital wastewater. Results of a particular hospital effluent show the presence of traces of heavy metals, high chlorine concentration and emerging contaminants such as non-steroidal anti-inflammatory drugs. The LC50 was of 5.49 % at 96 h. The cytotoxic, genotoxic and apoptotic biomarkers increase when fishes were exposed to wastewater (1/10 CL50) from hospital wastewater. This study emphasizes the importance of identifying and quantifying the effects of contaminants as pharmaceuticals, disinfectants and surfactants in order to design and implement an ecotoxicological plan.

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“…Enzymatic analyses.-For enzymatic analyses, supernatants from frozen sperm samples were obtained according to Gronczewska et al (2003). All assays were performed in duplicate and at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Sperm Metabolism and Biochemical Characteristics in First‐Time and Second‐Time Spawners of Farmed Atlantic Cod

et al. 2011

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We compared sperm biochemical characteristics of first‐generation (F1) hatchery‐reared broodstock of Atlantic cod Gadus morhua during the first and second spawnings to investigate potential improvements in sperm quality between the two spawning events. To gain further perspective on the sperm characteristics investigated, the values for farmed Atlantic cod were also compared with sperm characteristics of wild, experienced spawners that had been kept captive for 4 years. In particular, we investigated differences in the lactate dehydrogenase (glycolytic metabolism) and lipase (lipid catabolism) enzyme activities as well as sperm respiration rates and energy levels. Fertilization percentages for the F1 hatchery males at the first and second spawnings and for the wild broodstock were also obtained. The wild broodstock males were older and larger than the farmed males and produced sperm with higher energy levels, protein levels, and fertilization rates relative to the sperm of farmed naïve spawners. Our results showed no differences in characteristics between the first and second spawnings and seemed to contradict previous findings that demonstrated an improvement between first and second spawnings in captive wild Atlantic cod. Further investigations on sperm of hatchery‐reared Atlantic cod broodstock (e.g., sperm motility; fertilization rates of the same males) are necessary to confirm the present findings.

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Enzyme activities in fish spermatozoa with focus on lactate dehydrogenase isoenzymes from herring Clupea harengus

Cited by 23 publications

References 20 publications

Bioenergetics of fish spermatozoa during semen storage

Bioenergetics of fish spermatozoa during semen storage

Biomarkers of Cytotoxic, Genotoxic and Apoptotic Effects in Cyprinus carpio Exposed to Complex Mixture of Contaminants from Hospital Effluents

Sperm Metabolism and Biochemical Characteristics in First‐Time and Second‐Time Spawners of Farmed Atlantic Cod

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